Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990458230> ?p ?o ?g. }
- W1990458230 endingPage "54" @default.
- W1990458230 startingPage "47" @default.
- W1990458230 abstract "Chimeric genes containing Escherichia coli lacZ sequences are often used to characterize gene expression in yeast cells. By Northern analysis, we found that such genes produce multiple transcripts due to inefficient 3′-end formation. The same transcript pattern was found for two related chimeric genes when these genes were cloned separately into the commonly used vector, YIp5, and integrated into the yeast genome at two different locations. Each chimeric gene was composed of promoter and N-terminal coding regions from the yeast SSA1 or SSA2 genes fused in-frame to the lac operon. Transcripts were shown to initiate within the yeast promoter fragment, but transcript size indicated that 3′ ends were localized to three different regions: within the lac operon near the 3′ end of the lacZ gene; near a terminator region previously identified upstream of the URA3 gene in YIp5; and at the URA3 terminator region. Readthrough transcription of the URA3 promoter from upstream lac sequences decreased the basal activity of the URA3 promoter, although induced URA3 transcription levels were unaffected. This readthrough transcription also resulted in a novel, longer URA3 transcript." @default.
- W1990458230 created "2016-06-24" @default.
- W1990458230 creator A5043711354 @default.
- W1990458230 creator A5078652046 @default.
- W1990458230 creator A5088563796 @default.
- W1990458230 date "1991-07-01" @default.
- W1990458230 modified "2023-10-18" @default.
- W1990458230 title "Expression of lacZ gene fusions affects downstream transcription in yeast" @default.
- W1990458230 cites W1532986430 @default.
- W1990458230 cites W1590458072 @default.
- W1990458230 cites W1599809900 @default.
- W1990458230 cites W1754004588 @default.
- W1990458230 cites W1818430186 @default.
- W1990458230 cites W1830661192 @default.
- W1990458230 cites W1865160862 @default.
- W1990458230 cites W1874730733 @default.
- W1990458230 cites W1950655336 @default.
- W1990458230 cites W1968348380 @default.
- W1990458230 cites W1985382686 @default.
- W1990458230 cites W1995391727 @default.
- W1990458230 cites W1996010519 @default.
- W1990458230 cites W2003676968 @default.
- W1990458230 cites W2011715107 @default.
- W1990458230 cites W2014669590 @default.
- W1990458230 cites W2019262524 @default.
- W1990458230 cites W2045251366 @default.
- W1990458230 cites W2048380771 @default.
- W1990458230 cites W2057291831 @default.
- W1990458230 cites W2073923381 @default.
- W1990458230 cites W2074275408 @default.
- W1990458230 cites W2075307129 @default.
- W1990458230 cites W2086404403 @default.
- W1990458230 cites W2098089385 @default.
- W1990458230 cites W2098672021 @default.
- W1990458230 cites W2107239656 @default.
- W1990458230 cites W2117936243 @default.
- W1990458230 cites W2129131697 @default.
- W1990458230 cites W2132094774 @default.
- W1990458230 cites W2224465247 @default.
- W1990458230 cites W2260545369 @default.
- W1990458230 cites W2345024092 @default.
- W1990458230 cites W4234884599 @default.
- W1990458230 cites W4238099995 @default.
- W1990458230 cites W4240922224 @default.
- W1990458230 cites W4242709490 @default.
- W1990458230 cites W4249988281 @default.
- W1990458230 doi "https://doi.org/10.1016/0378-1119(91)90463-l" @default.
- W1990458230 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1717347" @default.
- W1990458230 hasPublicationYear "1991" @default.
- W1990458230 type Work @default.
- W1990458230 sameAs 1990458230 @default.
- W1990458230 citedByCount "7" @default.
- W1990458230 crossrefType "journal-article" @default.
- W1990458230 hasAuthorship W1990458230A5043711354 @default.
- W1990458230 hasAuthorship W1990458230A5078652046 @default.
- W1990458230 hasAuthorship W1990458230A5088563796 @default.
- W1990458230 hasConcept C101762097 @default.
- W1990458230 hasConcept C104317684 @default.
- W1990458230 hasConcept C116403925 @default.
- W1990458230 hasConcept C121332964 @default.
- W1990458230 hasConcept C1276947 @default.
- W1990458230 hasConcept C138885662 @default.
- W1990458230 hasConcept C150194340 @default.
- W1990458230 hasConcept C153911025 @default.
- W1990458230 hasConcept C170344550 @default.
- W1990458230 hasConcept C178046730 @default.
- W1990458230 hasConcept C179926584 @default.
- W1990458230 hasConcept C192245184 @default.
- W1990458230 hasConcept C203075996 @default.
- W1990458230 hasConcept C22744801 @default.
- W1990458230 hasConcept C2778137531 @default.
- W1990458230 hasConcept C41895202 @default.
- W1990458230 hasConcept C54355233 @default.
- W1990458230 hasConcept C547475151 @default.
- W1990458230 hasConcept C86803240 @default.
- W1990458230 hasConcept C91779695 @default.
- W1990458230 hasConceptScore W1990458230C101762097 @default.
- W1990458230 hasConceptScore W1990458230C104317684 @default.
- W1990458230 hasConceptScore W1990458230C116403925 @default.
- W1990458230 hasConceptScore W1990458230C121332964 @default.
- W1990458230 hasConceptScore W1990458230C1276947 @default.
- W1990458230 hasConceptScore W1990458230C138885662 @default.
- W1990458230 hasConceptScore W1990458230C150194340 @default.
- W1990458230 hasConceptScore W1990458230C153911025 @default.
- W1990458230 hasConceptScore W1990458230C170344550 @default.
- W1990458230 hasConceptScore W1990458230C178046730 @default.
- W1990458230 hasConceptScore W1990458230C179926584 @default.
- W1990458230 hasConceptScore W1990458230C192245184 @default.
- W1990458230 hasConceptScore W1990458230C203075996 @default.
- W1990458230 hasConceptScore W1990458230C22744801 @default.
- W1990458230 hasConceptScore W1990458230C2778137531 @default.
- W1990458230 hasConceptScore W1990458230C41895202 @default.
- W1990458230 hasConceptScore W1990458230C54355233 @default.
- W1990458230 hasConceptScore W1990458230C547475151 @default.
- W1990458230 hasConceptScore W1990458230C86803240 @default.
- W1990458230 hasConceptScore W1990458230C91779695 @default.
- W1990458230 hasIssue "1" @default.
- W1990458230 hasLocation W19904582301 @default.