Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990486534> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W1990486534 abstract "Abstract Background T4 RNA ligases 1 and 2 are useful tools for RNA analysis. Their use upstream of RNA analyses such as high-throughput RNA sequencing and microarrays has recently increased their importance. The truncated form of T4 RNA ligase 2, comprising amino acids 1-249 (T4 Rnl2tr), is an attractive tool for attachment of adapters or labels to RNA 3'-ends. Compared to T4 RNA ligase 1, T4 Rnl2tr has a decreased ability to ligate 5'-PO 4 ends in single-stranded RNA ligations, and compared to the full-length T4 Rnl2, the T4 Rnl2tr has an increased activity for joining 5'-adenylated adapters to RNA 3'-ends. The combination of these properties allows adapter attachment to RNA 3'-ends with reduced circularization and concatemerization of substrate RNA. Results With the aim of further reducing unwanted side ligation products, we substituted active site residues, known to be important for adenylyltransferase steps of the ligation reaction, in the context of T4 Rnl2tr. We characterized the variant ligases for the formation of unwanted ligation side products and for activity in the strand-joining reaction. Conclusions Our data demonstrate that lysine 227 is a key residue facilitating adenylyl transfer from adenylated ligation donor substrates to the ligase. This reversal of the second step of the ligation reaction correlates with the formation of unwanted ligation products. Thus, T4 Rn2tr mutants containing the K227Q mutation are useful for reducing undesired ligation products. We furthermore report optimal conditions for the use of these improved T4 Rnl2tr variants." @default.
- W1990486534 created "2016-06-24" @default.
- W1990486534 creator A5005695665 @default.
- W1990486534 creator A5006643791 @default.
- W1990486534 creator A5018560735 @default.
- W1990486534 creator A5019902933 @default.
- W1990486534 creator A5073616978 @default.
- W1990486534 date "2011-07-01" @default.
- W1990486534 modified "2023-10-06" @default.
- W1990486534 title "T4 RNA Ligase 2 truncated active site mutants: improved tools for RNA analysis" @default.
- W1990486534 cites W1622719173 @default.
- W1990486534 cites W1964798261 @default.
- W1990486534 cites W1979203600 @default.
- W1990486534 cites W1990502537 @default.
- W1990486534 cites W1991007428 @default.
- W1990486534 cites W2002146509 @default.
- W1990486534 cites W2004651171 @default.
- W1990486534 cites W2020859487 @default.
- W1990486534 cites W2064557873 @default.
- W1990486534 cites W2081497946 @default.
- W1990486534 cites W2102989692 @default.
- W1990486534 cites W2167557255 @default.
- W1990486534 doi "https://doi.org/10.1186/1472-6750-11-72" @default.
- W1990486534 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3149579" @default.
- W1990486534 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21722378" @default.
- W1990486534 hasPublicationYear "2011" @default.
- W1990486534 type Work @default.
- W1990486534 sameAs 1990486534 @default.
- W1990486534 citedByCount "51" @default.
- W1990486534 countsByYear W19904865342012 @default.
- W1990486534 countsByYear W19904865342013 @default.
- W1990486534 countsByYear W19904865342014 @default.
- W1990486534 countsByYear W19904865342015 @default.
- W1990486534 countsByYear W19904865342016 @default.
- W1990486534 countsByYear W19904865342017 @default.
- W1990486534 countsByYear W19904865342018 @default.
- W1990486534 countsByYear W19904865342019 @default.
- W1990486534 countsByYear W19904865342020 @default.
- W1990486534 countsByYear W19904865342021 @default.
- W1990486534 countsByYear W19904865342022 @default.
- W1990486534 countsByYear W19904865342023 @default.
- W1990486534 crossrefType "journal-article" @default.
- W1990486534 hasAuthorship W1990486534A5005695665 @default.
- W1990486534 hasAuthorship W1990486534A5006643791 @default.
- W1990486534 hasAuthorship W1990486534A5018560735 @default.
- W1990486534 hasAuthorship W1990486534A5019902933 @default.
- W1990486534 hasAuthorship W1990486534A5073616978 @default.
- W1990486534 hasBestOaLocation W19904865341 @default.
- W1990486534 hasConcept C104317684 @default.
- W1990486534 hasConcept C1342335 @default.
- W1990486534 hasConcept C153911025 @default.
- W1990486534 hasConcept C175114707 @default.
- W1990486534 hasConcept C179437574 @default.
- W1990486534 hasConcept C2909068217 @default.
- W1990486534 hasConcept C552990157 @default.
- W1990486534 hasConcept C55493867 @default.
- W1990486534 hasConcept C67705224 @default.
- W1990486534 hasConcept C76346623 @default.
- W1990486534 hasConcept C86803240 @default.
- W1990486534 hasConceptScore W1990486534C104317684 @default.
- W1990486534 hasConceptScore W1990486534C1342335 @default.
- W1990486534 hasConceptScore W1990486534C153911025 @default.
- W1990486534 hasConceptScore W1990486534C175114707 @default.
- W1990486534 hasConceptScore W1990486534C179437574 @default.
- W1990486534 hasConceptScore W1990486534C2909068217 @default.
- W1990486534 hasConceptScore W1990486534C552990157 @default.
- W1990486534 hasConceptScore W1990486534C55493867 @default.
- W1990486534 hasConceptScore W1990486534C67705224 @default.
- W1990486534 hasConceptScore W1990486534C76346623 @default.
- W1990486534 hasConceptScore W1990486534C86803240 @default.
- W1990486534 hasIssue "1" @default.
- W1990486534 hasLocation W19904865341 @default.
- W1990486534 hasLocation W19904865342 @default.
- W1990486534 hasLocation W19904865343 @default.
- W1990486534 hasLocation W19904865344 @default.
- W1990486534 hasOpenAccess W1990486534 @default.
- W1990486534 hasPrimaryLocation W19904865341 @default.
- W1990486534 hasRelatedWork W1990486534 @default.
- W1990486534 hasRelatedWork W2098211286 @default.
- W1990486534 hasRelatedWork W2160602216 @default.
- W1990486534 hasRelatedWork W2163096453 @default.
- W1990486534 hasRelatedWork W2283065081 @default.
- W1990486534 hasRelatedWork W2529556041 @default.
- W1990486534 hasRelatedWork W35665488 @default.
- W1990486534 hasRelatedWork W4243324746 @default.
- W1990486534 hasRelatedWork W4285796434 @default.
- W1990486534 hasRelatedWork W968389318 @default.
- W1990486534 hasVolume "11" @default.
- W1990486534 isParatext "false" @default.
- W1990486534 isRetracted "false" @default.
- W1990486534 magId "1990486534" @default.
- W1990486534 workType "article" @default.