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- W1990488028 abstract "Photochemical and enzymatic synthesis of malic acid from pyruvic acid and HCO3- was studied with malic enzyme (ME) and NADP+ photoreduction with ferredoxin-NADP+-reductase (FNR) by visible light photo-sensitization of the chlorophyll derivative, zinc chlorin-e6 (ZnChl-e6), in the presence of NADH as an electron-donating reagent and methylviologen (MV2+) as an electron-relay reagent. NADPH was produced by irradiation of the reactant solution containing NADH, ZnChl-e6, MV2+, NADP+ and FNR in Bis-Tris buffer (pH 8.0). After 180-min irradiation, 8.3 mmol·dm-3 NADPH was produced under the optimum conditions with 10 mmol·dm-3 of NADP+. The reduction ratio of NADP+ to NADPH was about 83%. Malic acid was produced by irradiation with visible light (>390 nm) of the reactant solution containing NADH, ZnChl-e6, methylviologen (MV2+), pyruvic acid, NaHCO3, NADP+, ferredoxin-NADP+-reductase (FNR) and ME. Malic acid production was 2.0 mmol·dm-3 after 3-h irradiation under the optimum conditions with NADH, ZnChl-e6, MV2+, FNR, pyruvic acid, NaHCO3, NADP+ and ME. The ratio of HCO3- and pyruvic acid to malic acid was about 20% under the optimum conditions." @default.
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- W1990488028 date "2007-01-01" @default.
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- W1990488028 title "Photochemical and Enzymatic Synthesis of Malic Acid from Pyruvic Acid and HCO<sub>3</sub><sup>-</sup> with Combination System of Zinc Chlorin-e<sub>6</sub> and Malic Enzyme in Aqueous Medium" @default.
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- W1990488028 doi "https://doi.org/10.1627/jpi.50.272" @default.
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