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- W1990512336 abstract "Abstract In the two-step Vitamin C fermentative production, its precursor 2-keto- l -gulonic acid (2-KLG) was synthesized by Ketogulonicigenium vulgare through co-culture with Bacillus megaterium . The rates of K. vulgare cell growth and 2-KLG production were closely related with B. megaterium concentration in the co-culture system. To enhance the 2-KLG production efficiency, a strategy of manipulating B. megaterium growth in the co-culture system and properly releasing its intracellular components was introduced. Lysozyme was used specifically to damage B. megaterium cell wall structure and subsequently inhibit its cell growth. When 10,000 U mL −1 lysozyme was fed to the co-culture system at 12 h, the growth rate of K. vulgare , sorbose consumption rate, and 2-KLG productivity could increase 27.4%, 37.1%, and 28.2%, respectively." @default.
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- W1990512336 date "2010-04-01" @default.
- W1990512336 modified "2023-09-27" @default.
- W1990512336 title "Manipulation of B. megaterium growth for efficient 2-KLG production by K. vulgare" @default.
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- W1990512336 doi "https://doi.org/10.1016/j.procbio.2009.11.016" @default.
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