FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990549237> ?p ?o ?g. }

• W1990549237 endingPage "580" @default.
• W1990549237 startingPage "571" @default.
• W1990549237 abstract "Despite extensive research in the differentiation of rodent ESCs into cardiomyocytes, there have been few studies of this process in primates. In this study, we examined the role of bone morphogenic protein-4 (BMP-4) to induce cardiomyocyte differentiation of cynomolgus monkey ESCs. To study the role of BMP-4, EBs were formed and cultured in Knockout Serum Replacement (KSR) medium containing BMP-4 for 8 days and subsequently seeded in gelatin-coated dishes for 20 days. It was found that ESCs differentiated into cardiomyocytes upon stimulation with BMP-4 in KSR medium, which resulted in a large fraction of beating EBs ( approximately 16%) and the upregulation of cardiac-specific proteins in a dose and time-dependent manner. In contrast, the addition of BMP-4 in FBS-containing medium resulted in a lower fraction of beating EBs ( approximately 6%). BMP-4 acted principally between mesendodermal and mesoderm progenitors and subsequently enhanced their expression. Ultrastructural observation revealed that beating EBs contained mature cardiomyocytes with sarcomeric structures. In addition, immunostaining, reverse transcription-polymerase chain reaction, and Western blotting for cardiac markers confirmed the increased differentiation of cardiomyocytes in these cultures. Moreover, electrophysiological studies demonstrated that the differentiated cardiomyocytes were electrically activated. These findings may be useful in developing effective culture conditions to differentiate cynomolgus monkey ESCs into cardiomyocytes for studying developmental biology and for regenerative medicine." @default.
• W1990549237 created "2016-06-24" @default.
• W1990549237 creator A5003899723 @default.
• W1990549237 creator A5004421810 @default.
• W1990549237 creator A5021677098 @default.
• W1990549237 creator A5033056518 @default.
• W1990549237 creator A5043801221 @default.
• W1990549237 creator A5044983234 @default.
• W1990549237 date "2006-11-30" @default.
• W1990549237 modified "2023-09-27" @default.
• W1990549237 title "Bone Morphogenetic Protein-4 Enhances Cardiomyocyte Differentiation of Cynomolgus Monkey ESCs in Knockout Serum Replacement Medium" @default.
• W1990549237 cites W1937940610 @default.
• W1990549237 cites W1969210306 @default.
• W1990549237 cites W1979608640 @default.
• W1990549237 cites W1981089517 @default.
• W1990549237 cites W1996151007 @default.
• W1990549237 cites W2004039221 @default.
• W1990549237 cites W2011077415 @default.
• W1990549237 cites W2013696266 @default.
• W1990549237 cites W2015301717 @default.
• W1990549237 cites W2016980442 @default.
• W1990549237 cites W2025239125 @default.
• W1990549237 cites W2026014504 @default.
• W1990549237 cites W2042447396 @default.
• W1990549237 cites W2045583901 @default.
• W1990549237 cites W2050753656 @default.
• W1990549237 cites W2068001725 @default.
• W1990549237 cites W2076396921 @default.
• W1990549237 cites W2077116679 @default.
• W1990549237 cites W2083312275 @default.
• W1990549237 cites W2085030176 @default.
• W1990549237 cites W2085306336 @default.
• W1990549237 cites W2090993605 @default.
• W1990549237 cites W2091099944 @default.
• W1990549237 cites W2100088611 @default.
• W1990549237 cites W2107141327 @default.
• W1990549237 cites W2107800478 @default.
• W1990549237 cites W2111643738 @default.
• W1990549237 cites W2116695601 @default.
• W1990549237 cites W2119288897 @default.
• W1990549237 cites W2123868981 @default.
• W1990549237 cites W2126170723 @default.
• W1990549237 cites W2126641307 @default.
• W1990549237 cites W2136071912 @default.
• W1990549237 cites W2153155418 @default.
• W1990549237 cites W2170560537 @default.
• W1990549237 cites W2317845988 @default.
• W1990549237 doi "https://doi.org/10.1634/stemcells.2006-0225" @default.
• W1990549237 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17138962" @default.
• W1990549237 hasPublicationYear "2006" @default.
• W1990549237 type Work @default.
• W1990549237 sameAs 1990549237 @default.
• W1990549237 citedByCount "28" @default.
• W1990549237 countsByYear W19905492372012 @default.
• W1990549237 countsByYear W19905492372013 @default.
• W1990549237 countsByYear W19905492372014 @default.
• W1990549237 countsByYear W19905492372015 @default.
• W1990549237 countsByYear W19905492372017 @default.
• W1990549237 countsByYear W19905492372018 @default.
• W1990549237 countsByYear W19905492372019 @default.
• W1990549237 countsByYear W19905492372022 @default.
• W1990549237 crossrefType "journal-article" @default.
• W1990549237 hasAuthorship W1990549237A5003899723 @default.
• W1990549237 hasAuthorship W1990549237A5004421810 @default.
• W1990549237 hasAuthorship W1990549237A5021677098 @default.
• W1990549237 hasAuthorship W1990549237A5033056518 @default.
• W1990549237 hasAuthorship W1990549237A5043801221 @default.
• W1990549237 hasAuthorship W1990549237A5044983234 @default.
• W1990549237 hasConcept C104317684 @default.
• W1990549237 hasConcept C116186050 @default.
• W1990549237 hasConcept C125349252 @default.
• W1990549237 hasConcept C145103041 @default.
• W1990549237 hasConcept C148738053 @default.
• W1990549237 hasConcept C153911025 @default.
• W1990549237 hasConcept C201750760 @default.
• W1990549237 hasConcept C202751555 @default.
• W1990549237 hasConcept C203014093 @default.
• W1990549237 hasConcept C204232928 @default.
• W1990549237 hasConcept C2780915607 @default.
• W1990549237 hasConcept C28328180 @default.
• W1990549237 hasConcept C30352789 @default.
• W1990549237 hasConcept C31507581 @default.
• W1990549237 hasConcept C4224716 @default.
• W1990549237 hasConcept C54355233 @default.
• W1990549237 hasConcept C86339819 @default.
• W1990549237 hasConcept C86803240 @default.
• W1990549237 hasConcept C95444343 @default.
• W1990549237 hasConceptScore W1990549237C104317684 @default.
• W1990549237 hasConceptScore W1990549237C116186050 @default.
• W1990549237 hasConceptScore W1990549237C125349252 @default.
• W1990549237 hasConceptScore W1990549237C145103041 @default.
• W1990549237 hasConceptScore W1990549237C148738053 @default.
• W1990549237 hasConceptScore W1990549237C153911025 @default.
• W1990549237 hasConceptScore W1990549237C201750760 @default.
• W1990549237 hasConceptScore W1990549237C202751555 @default.
• W1990549237 hasConceptScore W1990549237C203014093 @default.
• W1990549237 hasConceptScore W1990549237C204232928 @default.
• W1990549237 hasConceptScore W1990549237C2780915607 @default.

• next