Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990584294> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1990584294 endingPage "223a" @default.
- W1990584294 startingPage "223a" @default.
- W1990584294 abstract "The DNA mismatch repair (MMR) system is an essential component of the cellular DNA replication process that ensures high overall fidelity. The protein MutS initiates MMR by binding specifically to base-base mismatches and insertion-deletion mismatches in double stranded DNA. The active MutS dimer includes two ATP binding sites that hydrolyze ATP in both DNA bound and free states. ATP is also hydrolyzed as part of the MMR cascade involving MutS. Biochemical characterizations have shown that distinct nucleotides produce different interactions between MutS and mismatched DNA substrates, but the details of the conformations of MutS-mismatched DNA complexes under exposure to different nucleotides are not well known. We used single molecule fluorescence resonance energy transfer (smFRET) to study MutS from Thermus aquaticus in isolation and in complex with mismatched DNA substrates when exposed to a variety of different nucleotide conditions. We report results using intramolecular FRET from MutS or the DNA substrate as well as intermolecular FRET between MutS and the DNA. Our results allow MutS-DNA conformations resulting from specific nucleotide bound states to be characterized. We suggest possible roles for ATP cycling that could regulate the function of MutS in DNA MMR." @default.
- W1990584294 created "2016-06-24" @default.
- W1990584294 creator A5023990361 @default.
- W1990584294 creator A5080802224 @default.
- W1990584294 date "2011-02-01" @default.
- W1990584294 modified "2023-09-29" @default.
- W1990584294 title "Single Molecule FRET Study of Nucleotide Binding Effects on Muts Proteins" @default.
- W1990584294 doi "https://doi.org/10.1016/j.bpj.2010.12.1431" @default.
- W1990584294 hasPublicationYear "2011" @default.
- W1990584294 type Work @default.
- W1990584294 sameAs 1990584294 @default.
- W1990584294 citedByCount "0" @default.
- W1990584294 crossrefType "journal-article" @default.
- W1990584294 hasAuthorship W1990584294A5023990361 @default.
- W1990584294 hasAuthorship W1990584294A5080802224 @default.
- W1990584294 hasBestOaLocation W19905842941 @default.
- W1990584294 hasConcept C104317684 @default.
- W1990584294 hasConcept C121332964 @default.
- W1990584294 hasConcept C12554922 @default.
- W1990584294 hasConcept C134935766 @default.
- W1990584294 hasConcept C185581394 @default.
- W1990584294 hasConcept C185592680 @default.
- W1990584294 hasConcept C2756471 @default.
- W1990584294 hasConcept C2780883830 @default.
- W1990584294 hasConcept C2781440490 @default.
- W1990584294 hasConcept C2781455991 @default.
- W1990584294 hasConcept C512185932 @default.
- W1990584294 hasConcept C547475151 @default.
- W1990584294 hasConcept C552990157 @default.
- W1990584294 hasConcept C55493867 @default.
- W1990584294 hasConcept C60748783 @default.
- W1990584294 hasConcept C62520636 @default.
- W1990584294 hasConcept C73573662 @default.
- W1990584294 hasConcept C86803240 @default.
- W1990584294 hasConcept C91881484 @default.
- W1990584294 hasConcept C96305047 @default.
- W1990584294 hasConceptScore W1990584294C104317684 @default.
- W1990584294 hasConceptScore W1990584294C121332964 @default.
- W1990584294 hasConceptScore W1990584294C12554922 @default.
- W1990584294 hasConceptScore W1990584294C134935766 @default.
- W1990584294 hasConceptScore W1990584294C185581394 @default.
- W1990584294 hasConceptScore W1990584294C185592680 @default.
- W1990584294 hasConceptScore W1990584294C2756471 @default.
- W1990584294 hasConceptScore W1990584294C2780883830 @default.
- W1990584294 hasConceptScore W1990584294C2781440490 @default.
- W1990584294 hasConceptScore W1990584294C2781455991 @default.
- W1990584294 hasConceptScore W1990584294C512185932 @default.
- W1990584294 hasConceptScore W1990584294C547475151 @default.
- W1990584294 hasConceptScore W1990584294C552990157 @default.
- W1990584294 hasConceptScore W1990584294C55493867 @default.
- W1990584294 hasConceptScore W1990584294C60748783 @default.
- W1990584294 hasConceptScore W1990584294C62520636 @default.
- W1990584294 hasConceptScore W1990584294C73573662 @default.
- W1990584294 hasConceptScore W1990584294C86803240 @default.
- W1990584294 hasConceptScore W1990584294C91881484 @default.
- W1990584294 hasConceptScore W1990584294C96305047 @default.
- W1990584294 hasIssue "3" @default.
- W1990584294 hasLocation W19905842941 @default.
- W1990584294 hasOpenAccess W1990584294 @default.
- W1990584294 hasPrimaryLocation W19905842941 @default.
- W1990584294 hasRelatedWork W1976826236 @default.
- W1990584294 hasRelatedWork W1987471315 @default.
- W1990584294 hasRelatedWork W1988570895 @default.
- W1990584294 hasRelatedWork W1989451710 @default.
- W1990584294 hasRelatedWork W1992849304 @default.
- W1990584294 hasRelatedWork W2026240741 @default.
- W1990584294 hasRelatedWork W2052821060 @default.
- W1990584294 hasRelatedWork W2056197064 @default.
- W1990584294 hasRelatedWork W2060342115 @default.
- W1990584294 hasRelatedWork W2066212453 @default.
- W1990584294 hasRelatedWork W2071419223 @default.
- W1990584294 hasRelatedWork W2081826806 @default.
- W1990584294 hasRelatedWork W2094214607 @default.
- W1990584294 hasRelatedWork W2106826690 @default.
- W1990584294 hasRelatedWork W2116573367 @default.
- W1990584294 hasRelatedWork W2121473677 @default.
- W1990584294 hasRelatedWork W2325431138 @default.
- W1990584294 hasRelatedWork W2476052731 @default.
- W1990584294 hasRelatedWork W2584077286 @default.
- W1990584294 hasRelatedWork W3020937580 @default.
- W1990584294 hasVolume "100" @default.
- W1990584294 isParatext "false" @default.
- W1990584294 isRetracted "false" @default.
- W1990584294 magId "1990584294" @default.
- W1990584294 workType "article" @default.