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- W1990585396 abstract "A bacteriophage λ Ch4A clone containing a 22-kb rat DNA insert was isolated and found to contain a solitary tRNA Phe GAA gene and, 436 bp downstream of it, an Alu -like element. The nucleotide sequence of a 1141-bp DNA fragment containing these genes was determined. The rat tRNA Phe GAA gene, with the exception of an additional A in the extra arm, has a sequence identical to that of a rabbit liver tRNA Phe . The Alu -like element belongs to the rodent B2 family of short interspersed repetitive nucleotide sequences. This repetitive element, B2Phe, is flanked by 12-bp direct repeats, contains an internal split promoter (block A and block B) for RNA polymerase III and is devoid of an A-rich segment at the 3' end. Like other members of the B2 family, the B2Phe element presents 64% sequence homology with rat serine tRNA and contains a serine (GCT) anticodon. Both tRNA Phe GAA gene and B2Phe element were found to be transcriptionally active in HeLa cell and Xenopus oocyte nuclear extracts. The tRNA Phe gene transcripts were processed during the course of transcription to form mature-size tRNA Phe . The transcription efficiency of the B2Phe element was found to be an order of magnitude higher than that of the tRNA Phe gene. Competition experiments demonstrate that the B2Phe DNA can form a more stable transcription complex than the tRNA Phe gene and compete with it for binding of transcription factors" @default.
- W1990585396 created "2016-06-24" @default.
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- W1990585396 date "1988-09-01" @default.
- W1990585396 modified "2023-09-27" @default.
- W1990585396 title "Nucleotide sequence and transcription of a rat tRNAPhe gene and a neighboring Alu-like element" @default.
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- W1990585396 doi "https://doi.org/10.1016/0378-1119(88)90438-6" @default.
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