FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990592004> ?p ?o ?g. }

• W1990592004 endingPage "17246" @default.
• W1990592004 startingPage "17236" @default.
• W1990592004 abstract "Carbohydrates are generally considered to be poorly immunogenic. Therefore, new approaches for enhancing their immunogenicity are important for the development of carbohydrates as vaccine components. We hypothesized that conjugation of an l-rhamnose (Rha) moiety to a carbohydrate antigen would enhance the antigenicity of the antigen in mice possessing anti-Rha antibodies via an antibody-dependent antigen uptake mechanism. To explore this hypothesis, we synthesized a single-molecule three-component vaccine containing the GalNAc-O-Thr (Tn) tumor-specific antigen, a 20 amino acid helper T-cell epitope (YAF) derived from an outer-membrane protein of Neisseria meningitides, and a Rha moiety. The vaccine was synthesized by automated Fmoc-based solid-phase peptide synthesis and deacetylated by brief treatment with NaOMe. Groups of female BALB/c mice were immunized and boosted with Rha-ovalbumin (Rha-OVA) formulated with either TiterMax Gold or Sigma Adjuvant System for a period of 35 days in order to determine optimal conditions for generating anti-Rha titers in mice. Anti-Rha antibody titers were >100 fold higher in groups of mice immunized with Rha-OVA than in the control groups. Mice producing anti-Rha were challenged with Rha-YAF-Tn or YAF-Tn. Sera collected from the groups initially immunized with Rha-OVA and later challenged with Rha-YAF-Tn showed a 2-fold increase in anti-Tn titer at 1/100 serum dilution relative to mice not immunized with Rha-OVA. An in vitro T-cell proliferation study using cells primed with either Rha-YAF-Tn or YAF-Tn was done to examine possible differences in antigen uptake and presentation due to anti-Rha antibody and chemical modification. Proliferation of T cells was stimulated by a 10-fold lower antigen concentration in the presence of Rha antibodies. The results strongly suggest that T cells present in the spleen were presented with higher concentrations of Rha-YAF-Tn as a result of the presence of the anti-Rha antibodies." @default.
• W1990592004 created "2016-06-24" @default.
• W1990592004 creator A5000704778 @default.
• W1990592004 creator A5012445582 @default.
• W1990592004 creator A5022800371 @default.
• W1990592004 creator A5024958538 @default.
• W1990592004 creator A5029732152 @default.
• W1990592004 creator A5037018232 @default.
• W1990592004 date "2010-11-16" @default.
• W1990592004 modified "2023-10-08" @default.
• W1990592004 title "Synthesis of a Single-Molecule <scp>l</scp>-Rhamnose-Containing Three-Component Vaccine and Evaluation of Antigenicity in the Presence of Anti-<scp>l</scp>-Rhamnose Antibodies" @default.
• W1990592004 cites W1500498791 @default.
• W1990592004 cites W1500851417 @default.
• W1990592004 cites W1527811569 @default.
• W1990592004 cites W1866549327 @default.
• W1990592004 cites W1968834637 @default.
• W1990592004 cites W1986640672 @default.
• W1990592004 cites W1988183869 @default.
• W1990592004 cites W1995611890 @default.
• W1990592004 cites W1997462314 @default.
• W1990592004 cites W1998995307 @default.
• W1990592004 cites W2000699613 @default.
• W1990592004 cites W2007679990 @default.
• W1990592004 cites W2018940756 @default.
• W1990592004 cites W2021033320 @default.
• W1990592004 cites W2024930755 @default.
• W1990592004 cites W2025091989 @default.
• W1990592004 cites W2025342394 @default.
• W1990592004 cites W2026305391 @default.
• W1990592004 cites W2027957461 @default.
• W1990592004 cites W2036963376 @default.
• W1990592004 cites W2041327871 @default.
• W1990592004 cites W2046667705 @default.
• W1990592004 cites W2054183568 @default.
• W1990592004 cites W2054476963 @default.
• W1990592004 cites W2059674129 @default.
• W1990592004 cites W2059959414 @default.
• W1990592004 cites W2066863196 @default.
• W1990592004 cites W2073529249 @default.
• W1990592004 cites W2075692833 @default.
• W1990592004 cites W2075997187 @default.
• W1990592004 cites W2077463658 @default.
• W1990592004 cites W2082904820 @default.
• W1990592004 cites W2091016446 @default.
• W1990592004 cites W2094067775 @default.
• W1990592004 cites W2095248047 @default.
• W1990592004 cites W2099635508 @default.
• W1990592004 cites W2103233872 @default.
• W1990592004 cites W2127738393 @default.
• W1990592004 cites W2134220661 @default.
• W1990592004 cites W2135064782 @default.
• W1990592004 cites W2136383144 @default.
• W1990592004 cites W2156375502 @default.
• W1990592004 cites W2158440793 @default.
• W1990592004 cites W2165267755 @default.
• W1990592004 cites W2167582336 @default.
• W1990592004 cites W2167991675 @default.
• W1990592004 doi "https://doi.org/10.1021/ja107029z" @default.
• W1990592004 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21080675" @default.
• W1990592004 hasPublicationYear "2010" @default.
• W1990592004 type Work @default.
• W1990592004 sameAs 1990592004 @default.
• W1990592004 citedByCount "49" @default.
• W1990592004 countsByYear W19905920042012 @default.
• W1990592004 countsByYear W19905920042013 @default.
• W1990592004 countsByYear W19905920042014 @default.
• W1990592004 countsByYear W19905920042015 @default.
• W1990592004 countsByYear W19905920042016 @default.
• W1990592004 countsByYear W19905920042017 @default.
• W1990592004 countsByYear W19905920042018 @default.
• W1990592004 countsByYear W19905920042019 @default.
• W1990592004 countsByYear W19905920042020 @default.
• W1990592004 countsByYear W19905920042021 @default.
• W1990592004 countsByYear W19905920042022 @default.
• W1990592004 countsByYear W19905920042023 @default.
• W1990592004 crossrefType "journal-article" @default.
• W1990592004 hasAuthorship W1990592004A5000704778 @default.
• W1990592004 hasAuthorship W1990592004A5012445582 @default.
• W1990592004 hasAuthorship W1990592004A5022800371 @default.
• W1990592004 hasAuthorship W1990592004A5024958538 @default.
• W1990592004 hasAuthorship W1990592004A5029732152 @default.
• W1990592004 hasAuthorship W1990592004A5037018232 @default.
• W1990592004 hasConcept C100817775 @default.
• W1990592004 hasConcept C147483822 @default.
• W1990592004 hasConcept C153911025 @default.
• W1990592004 hasConcept C159654299 @default.
• W1990592004 hasConcept C185592680 @default.
• W1990592004 hasConcept C195616568 @default.
• W1990592004 hasConcept C203014093 @default.
• W1990592004 hasConcept C2776946954 @default.
• W1990592004 hasConcept C2777863537 @default.
• W1990592004 hasConcept C2779026604 @default.
• W1990592004 hasConcept C2779213145 @default.
• W1990592004 hasConcept C2780868878 @default.
• W1990592004 hasConcept C32611913 @default.
• W1990592004 hasConcept C55493867 @default.
• W1990592004 hasConcept C86803240 @default.
• W1990592004 hasConcept C89423630 @default.

• next