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- W1990614174 abstract "Abstract A method for lipid isolation is presented that is alternative to the traditional chloroform/methanol extraction methods. This new method allows lipid isolation by solvent elution of a dry column composed of a tissue sample, anhydrous sodium sulfate, and Celite 545 diatomaceous earth groud together. To isolate total lipids, the dry column is eluted with a mixture of dichloromethane/methanol (90∶10, v/v). Alternatively, the lipids may be isolated and simultaneously separated into neutral and polar fractions by a sequential elution procedure; neutral lipids free of polar lipids are eluted first with dichloromethane, followed by elution of polar lipids with the dichloromethane/methanol (90∶10) mixture. The two dry column methods‐isocratic or sequential elution‐were compared with the traditional chloroform/methanol methods by gravimetric, thin layer chromatographic and phosphorus analyses." @default.
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- W1990614174 date "1981-05-01" @default.
- W1990614174 modified "2023-09-29" @default.
- W1990614174 title "Dry column method for the quantitative extraction and simultaneous class separation of lipids from muscle tissue" @default.
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- W1990614174 doi "https://doi.org/10.1007/bf02534964" @default.
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