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- W1990622767 abstract "Abstract Plasmalogens (1‐ O ‐alk‐1′‐enyl‐2‐acyl‐ sn ‐glycero‐3‐phosphocholines and ‐phosphoethanolamines) are important constituents of spermatozoa membranes and possess significant antioxidative properties. This particularly holds as plasmalogens from spermatozoa also possess a very high content of highly unsaturated fatty acyl residues (especially 22:6). The organic spermatozoa extracts of two different ruminants (cattle and roe deer) were analyzed for their contents of characteristic choline plasmalogen oxidation products by matrix‐assisted laser desorption and ionization time‐of‐flight (MALDI‐TOF) mass spectrometry. It will be shown that 1‐hydroxy‐2‐docosahexaenoyl‐ sn ‐glycero‐3‐phosphocholine (LPC 22:6) and formyl‐LPC 22:6 are reliable measures of lipid oxidation of spermatozoa and allow, accordingly, conclusions about the storage conditions. All data on spermatozoa were also confirmed by the investigation of the oxidation behavior of selected reference compounds. It will be shown that, equally if plasmalogens or diacyl PC species are used, oxidation takes place primarily at the double bond next to the glycerol backbone. These data were additionally confirmed by recording the corresponding post source decay (PSD) fragment ion spectra." @default.
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- W1990622767 date "2007-08-24" @default.
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- W1990622767 title "Characteristic Oxidation Products of Choline Plasmalogens are Detectable in Cattle and Roe Deer Spermatozoa by MALDI-TOF Mass Spectrometry" @default.
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- W1990622767 doi "https://doi.org/10.1007/s11745-007-3108-7" @default.
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