Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990675737> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W1990675737 endingPage "558" @default.
- W1990675737 startingPage "548" @default.
- W1990675737 abstract "Time-dependent 31P saturation-transfer studies were conducted with the Cd2+-activated form of muscle phosphoglucomutase to probe the origin of the 100-fold difference between its catalytic efficiency (in terms of kcat) and that of the more efficient Mg2+-activated enzyme. The present paper describes the equilibrium mixture of phosphoglucomutase and its substrate/product pair when the concentration of the Cd2+ enzyme approaches that of the substrate and how the nine-spin 31P NMR system provided by this mixture was treated. It shows that the presence of abortive complexes is not a significant factor in the reduced activity of the Cd2+ enzyme since the complex of the dephosphoenzyme and glucose 1,6-bisphosphate, which accounts for a large majority of the enzyme present at equilibrium, is catalytically competent. It also shows that rate constants for saturation transfer obtained at three different ratios of enzyme to free substrate are mutually compatible. These constants, which were measured at chemical equilibrium, can be used to provide a quantitative kinetic rationale for the reduced steady-state activity elicited by Cd2+ relative to Mg2+ [cf. Ray, W.J., Post, C.B., & Puvathingal, J.M. (1989) Biochemistry (following paper in this issue)]. They also provide minimal estimates of 350 and 150 s-1 for the rate constants describing (PO3-) transfer from the Cd2+ phosphoenzyme to the 6-position of bound glucose 1-phosphate and to the 1-position of bound glucose 6-phosphate, respectively. These minimal estimates are compared with analogous estimates for the Mg2+ and Li+ forms of the enzyme in the accompanying paper." @default.
- W1990675737 created "2016-06-24" @default.
- W1990675737 creator A5018903804 @default.
- W1990675737 creator A5068687236 @default.
- W1990675737 creator A5090905969 @default.
- W1990675737 date "1989-01-24" @default.
- W1990675737 modified "2023-09-25" @default.
- W1990675737 title "Time-dependent phosphorus-31 saturation transfer in the phosphoglucomutase reaction. Characterization of the spin system for the cadmium(II) enzyme and evaluation of rate constants for the transfer process" @default.
- W1990675737 cites W115421876 @default.
- W1990675737 cites W122711211 @default.
- W1990675737 cites W1489869742 @default.
- W1990675737 cites W1493924526 @default.
- W1990675737 cites W1508687943 @default.
- W1990675737 cites W1509843894 @default.
- W1990675737 cites W1657598186 @default.
- W1990675737 cites W1977993266 @default.
- W1990675737 cites W1984673052 @default.
- W1990675737 cites W1988031381 @default.
- W1990675737 cites W1997577650 @default.
- W1990675737 cites W2002952625 @default.
- W1990675737 cites W2011940822 @default.
- W1990675737 cites W2014090403 @default.
- W1990675737 cites W2038503862 @default.
- W1990675737 cites W2055557864 @default.
- W1990675737 cites W2077191757 @default.
- W1990675737 cites W2083741754 @default.
- W1990675737 doi "https://doi.org/10.1021/bi00428a021" @default.
- W1990675737 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2523728" @default.
- W1990675737 hasPublicationYear "1989" @default.
- W1990675737 type Work @default.
- W1990675737 sameAs 1990675737 @default.
- W1990675737 citedByCount "17" @default.
- W1990675737 crossrefType "journal-article" @default.
- W1990675737 hasAuthorship W1990675737A5018903804 @default.
- W1990675737 hasAuthorship W1990675737A5068687236 @default.
- W1990675737 hasAuthorship W1990675737A5090905969 @default.
- W1990675737 hasConcept C10710636 @default.
- W1990675737 hasConcept C111368507 @default.
- W1990675737 hasConcept C114614502 @default.
- W1990675737 hasConcept C121332964 @default.
- W1990675737 hasConcept C127313418 @default.
- W1990675737 hasConcept C147789679 @default.
- W1990675737 hasConcept C148898269 @default.
- W1990675737 hasConcept C181199279 @default.
- W1990675737 hasConcept C185592680 @default.
- W1990675737 hasConcept C2776165552 @default.
- W1990675737 hasConcept C2777289219 @default.
- W1990675737 hasConcept C33923547 @default.
- W1990675737 hasConcept C41183919 @default.
- W1990675737 hasConcept C55493867 @default.
- W1990675737 hasConcept C56856141 @default.
- W1990675737 hasConcept C62520636 @default.
- W1990675737 hasConcept C71240020 @default.
- W1990675737 hasConcept C93391505 @default.
- W1990675737 hasConcept C9930424 @default.
- W1990675737 hasConceptScore W1990675737C10710636 @default.
- W1990675737 hasConceptScore W1990675737C111368507 @default.
- W1990675737 hasConceptScore W1990675737C114614502 @default.
- W1990675737 hasConceptScore W1990675737C121332964 @default.
- W1990675737 hasConceptScore W1990675737C127313418 @default.
- W1990675737 hasConceptScore W1990675737C147789679 @default.
- W1990675737 hasConceptScore W1990675737C148898269 @default.
- W1990675737 hasConceptScore W1990675737C181199279 @default.
- W1990675737 hasConceptScore W1990675737C185592680 @default.
- W1990675737 hasConceptScore W1990675737C2776165552 @default.
- W1990675737 hasConceptScore W1990675737C2777289219 @default.
- W1990675737 hasConceptScore W1990675737C33923547 @default.
- W1990675737 hasConceptScore W1990675737C41183919 @default.
- W1990675737 hasConceptScore W1990675737C55493867 @default.
- W1990675737 hasConceptScore W1990675737C56856141 @default.
- W1990675737 hasConceptScore W1990675737C62520636 @default.
- W1990675737 hasConceptScore W1990675737C71240020 @default.
- W1990675737 hasConceptScore W1990675737C93391505 @default.
- W1990675737 hasConceptScore W1990675737C9930424 @default.
- W1990675737 hasIssue "2" @default.
- W1990675737 hasLocation W19906757371 @default.
- W1990675737 hasLocation W19906757372 @default.
- W1990675737 hasOpenAccess W1990675737 @default.
- W1990675737 hasPrimaryLocation W19906757371 @default.
- W1990675737 hasRelatedWork W1991418241 @default.
- W1990675737 hasRelatedWork W1992165385 @default.
- W1990675737 hasRelatedWork W2025788670 @default.
- W1990675737 hasRelatedWork W2029587448 @default.
- W1990675737 hasRelatedWork W2044180956 @default.
- W1990675737 hasRelatedWork W2062028372 @default.
- W1990675737 hasRelatedWork W2237717580 @default.
- W1990675737 hasRelatedWork W2317998381 @default.
- W1990675737 hasRelatedWork W2370792838 @default.
- W1990675737 hasRelatedWork W4244160061 @default.
- W1990675737 hasVolume "28" @default.
- W1990675737 isParatext "false" @default.
- W1990675737 isRetracted "false" @default.
- W1990675737 magId "1990675737" @default.
- W1990675737 workType "article" @default.