FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990692271> ?p ?o ?g. }

• W1990692271 endingPage "R31" @default.
• W1990692271 startingPage "R31" @default.
• W1990692271 abstract "Peroxisome proliferator-activated receptor γ (PPARγ) is a nuclear receptor involved in the regulation of many cellular processes. We and others have previously shown that PPARγ activators display anti-inflammatory and chondroprotective properties in vitro and improve the clinical course and histopathological features in an experimental animal model of osteoarthritis (OA). However, the expression and regulation of PPARγ expression in cartilage are poorly defined. This study was undertaken to investigate the quantitative expression and distribution of PPARγ in normal and OA cartilage and to evaluate the effect of IL-1β, a prominent cytokine in OA, on PPARγ expression in cultured chondrocytes. Immunohistochemical analysis revealed that the levels of PPARγ protein expression were significantly lower in OA cartilage than in normal cartilage. Using real-time RT-PCR, we demonstrated that PPARγ1 mRNA levels were about 10-fold higher than PPARγ2 mRNA levels, and that only PPARγ1 was differentially expressed: its levels in OA cartilage was 2.4-fold lower than in normal cartilage (p < 0.001). IL-1 treatment of OA chondrocytes downregulated PPARγ1 expression in a dose- and time-dependent manner. This effect probably occurred at the transcriptional level, because IL-1 decreases both PPARγ1 mRNA expression and PPARγ1 promoter activity. TNF-α, IL-17, and prostaglandin E2 (PGE2), which are involved in the pathogenesis of OA, also downregulated PPARγ1 expression. Specific inhibitors of the mitogen-activated protein kinases (MAPKs) p38 (SB203580) and c-Jun N-terminal kinase (SP600125), but not of extracellular signal-regulated kinase (PD98059), prevented IL-1-induced downregulation of PPARγ1 expression. Similarly, inhibitors of NF-κB signaling (pyrrolidine dithiocarbamate, MG-132, and SN-50) abolished the suppressive effect of IL-1. Thus, our study demonstrated that PPARγ1 is downregulated in OA cartilage. The pro-inflammatory cytokine IL-1 may be responsible for this downregulation via a mechanism involving activation of the MAPKs (p38 and JNK) and NF-κB signaling pathways. The IL-1-induced downregulation of PPARγ expression might be a new and additional important process by which IL-1 promotes articular inflammation and cartilage degradation." @default.
• W1990692271 created "2016-06-24" @default.
• W1990692271 creator A5026711692 @default.
• W1990692271 creator A5039776422 @default.
• W1990692271 creator A5051212787 @default.
• W1990692271 creator A5052591744 @default.
• W1990692271 creator A5053214598 @default.
• W1990692271 creator A5083125761 @default.
• W1990692271 creator A5088257611 @default.
• W1990692271 date "2007-01-01" @default.
• W1990692271 modified "2023-10-05" @default.
• W1990692271 title "Peroxisome proliferator-activated receptor γ1 expression is diminished in human osteoarthritic cartilage and is downregulated by interleukin-1β in articular chondrocytes" @default.
• W1990692271 cites W1543433813 @default.
• W1990692271 cites W1568585026 @default.
• W1990692271 cites W1639924204 @default.
• W1990692271 cites W1659730200 @default.
• W1990692271 cites W1718688340 @default.
• W1990692271 cites W1764850065 @default.
• W1990692271 cites W1964073342 @default.
• W1990692271 cites W1970968225 @default.
• W1990692271 cites W1982589867 @default.
• W1990692271 cites W1987601141 @default.
• W1990692271 cites W1992724479 @default.
• W1990692271 cites W2003956312 @default.
• W1990692271 cites W2008802313 @default.
• W1990692271 cites W2009361993 @default.
• W1990692271 cites W2009524387 @default.
• W1990692271 cites W2027870903 @default.
• W1990692271 cites W2028607361 @default.
• W1990692271 cites W2033701197 @default.
• W1990692271 cites W2035458523 @default.
• W1990692271 cites W2038083756 @default.
• W1990692271 cites W2040003495 @default.
• W1990692271 cites W2047982128 @default.
• W1990692271 cites W2052893433 @default.
• W1990692271 cites W2056146193 @default.
• W1990692271 cites W2058147305 @default.
• W1990692271 cites W2060021617 @default.
• W1990692271 cites W2062676485 @default.
• W1990692271 cites W2065667627 @default.
• W1990692271 cites W2065742642 @default.
• W1990692271 cites W2069137207 @default.
• W1990692271 cites W2072960798 @default.
• W1990692271 cites W2073298486 @default.
• W1990692271 cites W2073512971 @default.
• W1990692271 cites W2081050068 @default.
• W1990692271 cites W2084986398 @default.
• W1990692271 cites W2087090665 @default.
• W1990692271 cites W2087120324 @default.
• W1990692271 cites W2092519834 @default.
• W1990692271 cites W2094298080 @default.
• W1990692271 cites W2114840351 @default.
• W1990692271 cites W2132070369 @default.
• W1990692271 cites W2140014359 @default.
• W1990692271 cites W2146035452 @default.
• W1990692271 cites W2148649000 @default.
• W1990692271 cites W2152217106 @default.
• W1990692271 cites W2152685208 @default.
• W1990692271 cites W2161481279 @default.
• W1990692271 cites W2169224393 @default.
• W1990692271 cites W2186067809 @default.
• W1990692271 cites W2400587276 @default.
• W1990692271 cites W2408915738 @default.
• W1990692271 doi "https://doi.org/10.1186/ar2151" @default.
• W1990692271 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4061960" @default.
• W1990692271 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17386086" @default.
• W1990692271 hasPublicationYear "2007" @default.
• W1990692271 type Work @default.
• W1990692271 sameAs 1990692271 @default.
• W1990692271 citedByCount "68" @default.
• W1990692271 countsByYear W19906922712012 @default.
• W1990692271 countsByYear W19906922712013 @default.
• W1990692271 countsByYear W19906922712014 @default.
• W1990692271 countsByYear W19906922712015 @default.
• W1990692271 countsByYear W19906922712016 @default.
• W1990692271 countsByYear W19906922712018 @default.
• W1990692271 countsByYear W19906922712019 @default.
• W1990692271 countsByYear W19906922712020 @default.
• W1990692271 countsByYear W19906922712021 @default.
• W1990692271 countsByYear W19906922712022 @default.
• W1990692271 countsByYear W19906922712023 @default.
• W1990692271 crossrefType "journal-article" @default.
• W1990692271 hasAuthorship W1990692271A5026711692 @default.
• W1990692271 hasAuthorship W1990692271A5039776422 @default.
• W1990692271 hasAuthorship W1990692271A5051212787 @default.
• W1990692271 hasAuthorship W1990692271A5052591744 @default.
• W1990692271 hasAuthorship W1990692271A5053214598 @default.
• W1990692271 hasAuthorship W1990692271A5083125761 @default.
• W1990692271 hasAuthorship W1990692271A5088257611 @default.
• W1990692271 hasBestOaLocation W19906922711 @default.
• W1990692271 hasConcept C105702510 @default.
• W1990692271 hasConcept C126322002 @default.
• W1990692271 hasConcept C134018914 @default.
• W1990692271 hasConcept C142724271 @default.
• W1990692271 hasConcept C170493617 @default.
• W1990692271 hasConcept C184235292 @default.
• W1990692271 hasConcept C185592680 @default.
• W1990692271 hasConcept C187345961 @default.

• next