FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990695156> ?p ?o ?g. }

• W1990695156 endingPage "1482" @default.
• W1990695156 startingPage "1476" @default.
• W1990695156 abstract "The effect of prostaglandin E2 (PGE2) on osteoclast (OC) differentiation is unclear, either stimulator or inhibitor, depending on the in vitro system used. This probably reflects indirect mechanisms through intermediate cells. We have investigated the direct effect of PGE2 on human OC differentiation from cord blood monocytes (CBMs) in the absence of stromal cells. Macrophages and multinucleated cells (MNCs) resembling OCs form in cultures of CBMs stimulated by 1,25-dihydroxyvitamin D3. In the present study, CBMs were cultured for 3 weeks, as previously described, in the presence or absence of PGE2. The number of MNCs was significantly reduced in the presence of PGE2 as was the proliferation of cultured CBMs, assessed on day 7. Immunohistochemistry was performed to evaluate macrophage markers (CD11b and CD14) and OC marker (beta3-chain). PGE2 significantly increased the numbers of CD11b-positive and CD14-positive cells, whereas the number of beta3-chain-positive cells was significantly decreased. beta3-Chain, c-fos, and human calcitonin receptor (h-CTR) messenger RNA (mRNA) expressions were evaluated by reverse transcription-PCR with RNA extracted from cultured CBMs. In the presence of PGE2, expression of beta3-chain and c-fos mRNA was reduced from the first week of culture. h-CTR mRNA expression was also reduced, and only the h-CTR1 isoform was detected in the presence of PGE2. In addition, when PGE2 was added only during the last week of culture, when no CBM proliferation occurred, the number of CD11b- and beta3-positive cells was unchanged compared to that in the control culture, as were the proportion of MNCs, the fusion index, and the expression of c-fos mRNA. In conclusion, our results suggest that PGE2 has an inhibitory effect on human OC differentiation from CBMs, possibly by reducing precursor proliferation in these cultures. We also hypothesize that PGE2 may reduce OC differentiation by increasing the proportion of precursor cells that differentiate into macrophages. In addition, this may be the result of inhibition of the c-fos expression in CBMs." @default.
• W1990695156 created "2016-06-24" @default.
• W1990695156 creator A5001947070 @default.
• W1990695156 creator A5020653419 @default.
• W1990695156 creator A5031821068 @default.
• W1990695156 creator A5054080498 @default.
• W1990695156 creator A5071455176 @default.
• W1990695156 creator A5078290825 @default.
• W1990695156 creator A5085754564 @default.
• W1990695156 date "1997-04-01" @default.
• W1990695156 modified "2023-10-02" @default.
• W1990695156 title "Effects of Prostaglandins on Human Hematopoietic Osteoclast Precursors" @default.
• W1990695156 cites W1966404053 @default.
• W1990695156 cites W1974261403 @default.
• W1990695156 cites W1974402772 @default.
• W1990695156 cites W1980243802 @default.
• W1990695156 cites W1982749890 @default.
• W1990695156 cites W1992088885 @default.
• W1990695156 cites W1993064864 @default.
• W1990695156 cites W2001605936 @default.
• W1990695156 cites W2004118573 @default.
• W1990695156 cites W2012268307 @default.
• W1990695156 cites W2023694966 @default.
• W1990695156 cites W2026862825 @default.
• W1990695156 cites W2028335715 @default.
• W1990695156 cites W2034804535 @default.
• W1990695156 cites W2036639134 @default.
• W1990695156 cites W2060962201 @default.
• W1990695156 cites W2089513211 @default.
• W1990695156 cites W2090795226 @default.
• W1990695156 cites W2094192333 @default.
• W1990695156 cites W2102285032 @default.
• W1990695156 cites W2103429229 @default.
• W1990695156 cites W2107927117 @default.
• W1990695156 cites W2125710129 @default.
• W1990695156 cites W2130183308 @default.
• W1990695156 cites W2140473585 @default.
• W1990695156 cites W2147706180 @default.
• W1990695156 cites W2324583578 @default.
• W1990695156 cites W2397123654 @default.
• W1990695156 cites W4294216491 @default.
• W1990695156 doi "https://doi.org/10.1210/endo.138.4.5070" @default.
• W1990695156 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9075705" @default.
• W1990695156 hasPublicationYear "1997" @default.
• W1990695156 type Work @default.
• W1990695156 sameAs 1990695156 @default.
• W1990695156 citedByCount "18" @default.
• W1990695156 countsByYear W19906951562012 @default.
• W1990695156 countsByYear W19906951562022 @default.
• W1990695156 countsByYear W19906951562023 @default.
• W1990695156 crossrefType "journal-article" @default.
• W1990695156 hasAuthorship W1990695156A5001947070 @default.
• W1990695156 hasAuthorship W1990695156A5020653419 @default.
• W1990695156 hasAuthorship W1990695156A5031821068 @default.
• W1990695156 hasAuthorship W1990695156A5054080498 @default.
• W1990695156 hasAuthorship W1990695156A5071455176 @default.
• W1990695156 hasAuthorship W1990695156A5078290825 @default.
• W1990695156 hasAuthorship W1990695156A5085754564 @default.
• W1990695156 hasBestOaLocation W19906951561 @default.
• W1990695156 hasConcept C104317684 @default.
• W1990695156 hasConcept C105580179 @default.
• W1990695156 hasConcept C109159458 @default.
• W1990695156 hasConcept C126322002 @default.
• W1990695156 hasConcept C134018914 @default.
• W1990695156 hasConcept C153911025 @default.
• W1990695156 hasConcept C16930146 @default.
• W1990695156 hasConcept C171779818 @default.
• W1990695156 hasConcept C202751555 @default.
• W1990695156 hasConcept C2776033226 @default.
• W1990695156 hasConcept C2777956040 @default.
• W1990695156 hasConcept C2778513237 @default.
• W1990695156 hasConcept C28328180 @default.
• W1990695156 hasConcept C553184892 @default.
• W1990695156 hasConcept C55493867 @default.
• W1990695156 hasConcept C71924100 @default.
• W1990695156 hasConcept C86803240 @default.
• W1990695156 hasConcept C95444343 @default.
• W1990695156 hasConceptScore W1990695156C104317684 @default.
• W1990695156 hasConceptScore W1990695156C105580179 @default.
• W1990695156 hasConceptScore W1990695156C109159458 @default.
• W1990695156 hasConceptScore W1990695156C126322002 @default.
• W1990695156 hasConceptScore W1990695156C134018914 @default.
• W1990695156 hasConceptScore W1990695156C153911025 @default.
• W1990695156 hasConceptScore W1990695156C16930146 @default.
• W1990695156 hasConceptScore W1990695156C171779818 @default.
• W1990695156 hasConceptScore W1990695156C202751555 @default.
• W1990695156 hasConceptScore W1990695156C2776033226 @default.
• W1990695156 hasConceptScore W1990695156C2777956040 @default.
• W1990695156 hasConceptScore W1990695156C2778513237 @default.
• W1990695156 hasConceptScore W1990695156C28328180 @default.
• W1990695156 hasConceptScore W1990695156C553184892 @default.
• W1990695156 hasConceptScore W1990695156C55493867 @default.
• W1990695156 hasConceptScore W1990695156C71924100 @default.
• W1990695156 hasConceptScore W1990695156C86803240 @default.
• W1990695156 hasConceptScore W1990695156C95444343 @default.
• W1990695156 hasIssue "4" @default.
• W1990695156 hasLocation W19906951561 @default.
• W1990695156 hasLocation W19906951562 @default.

• next