Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990804759> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W1990804759 abstract "During scanning mutagenesis of the S6 region of BK channels, we found a deep pore residue (M314 in hSlo) with interesting gating phenotypes. At pH7, the histidine substitution at this position (M314H) gave rise to currents with significantly slower deactivation kinetics than the wild-type BK. Deactivation became even slower with intracellular pH6 and pH5. To test whether this observation is an effect due to side-chain charges, we made three other charge-substituting mutants, M314K, M314E and M314D. For M314K, deactivation kinetics of the currents at pH7 was similar to that of M314H at pH5. This is consistent with the fact that the lysine (K) side-chain has a more basic pKa than that of histidine (H). For both M314H and M314K, increasing proton concentration (lowering pH) made the deactivation slower while reducing proton concentration (increasing pH) made the deactivation faster. For M314E, deactivation kinetics of the currents was fast at pH5 and became slower as the intracellular proton concentration was reduced to pH6, 7, 8, 9 and 10. The M314D channels could stay open at negative potentials in 0 Ca2+, at pH7, 8, 9 and 10. The most effective way to close the M314D channels was to increase the intracellular proton concentration to pH5. The results from all the charge-substituted mutants (M314H, M314K, M314E and M314D) support the idea that neutralizing the side-chain at the 314 position helped the channels close while charging the side-chain made it difficult for the channels to close. We propose that the charged form of the substituted residues may prefer the polar environment of the pore and stabilize the open state. The 314 residue may become more exposed to the pore as the channels transition from the closed to the open conformation." @default.
- W1990804759 created "2016-06-24" @default.
- W1990804759 creator A5051410023 @default.
- W1990804759 creator A5057874053 @default.
- W1990804759 date "2010-01-01" @default.
- W1990804759 modified "2023-09-29" @default.
- W1990804759 title "Charge Substitution of a Deep-Pore Residue Suggests Structural Rearrangements During BK Channel Activation" @default.
- W1990804759 doi "https://doi.org/10.1016/j.bpj.2009.12.689" @default.
- W1990804759 hasPublicationYear "2010" @default.
- W1990804759 type Work @default.
- W1990804759 sameAs 1990804759 @default.
- W1990804759 citedByCount "0" @default.
- W1990804759 crossrefType "journal-article" @default.
- W1990804759 hasAuthorship W1990804759A5051410023 @default.
- W1990804759 hasAuthorship W1990804759A5057874053 @default.
- W1990804759 hasBestOaLocation W19908047591 @default.
- W1990804759 hasConcept C104317684 @default.
- W1990804759 hasConcept C121332964 @default.
- W1990804759 hasConcept C12554922 @default.
- W1990804759 hasConcept C143065580 @default.
- W1990804759 hasConcept C148898269 @default.
- W1990804759 hasConcept C16318435 @default.
- W1990804759 hasConcept C178790620 @default.
- W1990804759 hasConcept C185592680 @default.
- W1990804759 hasConcept C194544171 @default.
- W1990804759 hasConcept C204921945 @default.
- W1990804759 hasConcept C2778460671 @default.
- W1990804759 hasConcept C2781338088 @default.
- W1990804759 hasConcept C515207424 @default.
- W1990804759 hasConcept C521977710 @default.
- W1990804759 hasConcept C54516573 @default.
- W1990804759 hasConcept C55493867 @default.
- W1990804759 hasConcept C62520636 @default.
- W1990804759 hasConcept C71240020 @default.
- W1990804759 hasConcept C79879829 @default.
- W1990804759 hasConcept C8010536 @default.
- W1990804759 hasConcept C86803240 @default.
- W1990804759 hasConceptScore W1990804759C104317684 @default.
- W1990804759 hasConceptScore W1990804759C121332964 @default.
- W1990804759 hasConceptScore W1990804759C12554922 @default.
- W1990804759 hasConceptScore W1990804759C143065580 @default.
- W1990804759 hasConceptScore W1990804759C148898269 @default.
- W1990804759 hasConceptScore W1990804759C16318435 @default.
- W1990804759 hasConceptScore W1990804759C178790620 @default.
- W1990804759 hasConceptScore W1990804759C185592680 @default.
- W1990804759 hasConceptScore W1990804759C194544171 @default.
- W1990804759 hasConceptScore W1990804759C204921945 @default.
- W1990804759 hasConceptScore W1990804759C2778460671 @default.
- W1990804759 hasConceptScore W1990804759C2781338088 @default.
- W1990804759 hasConceptScore W1990804759C515207424 @default.
- W1990804759 hasConceptScore W1990804759C521977710 @default.
- W1990804759 hasConceptScore W1990804759C54516573 @default.
- W1990804759 hasConceptScore W1990804759C55493867 @default.
- W1990804759 hasConceptScore W1990804759C62520636 @default.
- W1990804759 hasConceptScore W1990804759C71240020 @default.
- W1990804759 hasConceptScore W1990804759C79879829 @default.
- W1990804759 hasConceptScore W1990804759C8010536 @default.
- W1990804759 hasConceptScore W1990804759C86803240 @default.
- W1990804759 hasLocation W19908047591 @default.
- W1990804759 hasOpenAccess W1990804759 @default.
- W1990804759 hasPrimaryLocation W19908047591 @default.
- W1990804759 hasRelatedWork W135965306 @default.
- W1990804759 hasRelatedWork W1518994044 @default.
- W1990804759 hasRelatedWork W1903479391 @default.
- W1990804759 hasRelatedWork W1966690002 @default.
- W1990804759 hasRelatedWork W1971393605 @default.
- W1990804759 hasRelatedWork W1982934768 @default.
- W1990804759 hasRelatedWork W1993448210 @default.
- W1990804759 hasRelatedWork W1994285472 @default.
- W1990804759 hasRelatedWork W1996845992 @default.
- W1990804759 hasRelatedWork W1998401151 @default.
- W1990804759 hasRelatedWork W1998975947 @default.
- W1990804759 hasRelatedWork W2006370706 @default.
- W1990804759 hasRelatedWork W2054008220 @default.
- W1990804759 hasRelatedWork W2067642658 @default.
- W1990804759 hasRelatedWork W2077485181 @default.
- W1990804759 hasRelatedWork W2083123834 @default.
- W1990804759 hasRelatedWork W2110476053 @default.
- W1990804759 hasRelatedWork W2129095257 @default.
- W1990804759 hasRelatedWork W2129112892 @default.
- W1990804759 hasRelatedWork W2140404712 @default.
- W1990804759 isParatext "false" @default.
- W1990804759 isRetracted "false" @default.
- W1990804759 magId "1990804759" @default.
- W1990804759 workType "article" @default.