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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990806032> ?p ?o ?g. }

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• W1990806032 abstract "Two forms of recombinant trichosanthin (rTCS) were synthesized in high levels in Escherichia coli by putting the TCS cDNA under the control of a T7 RNA polymerase-directed promoter. Purification schemes were developed to isolate the recombinant protein from both soluble and insoluble fractions. Form I rTCS possessed the mature TCS sequence and had similar biological activities as the natural protein. Its IC50 was approximately 0.13 nM in an in vitro rabbit reticulocyte translational system and a dose of around 35 micrograms protein per 25 g body weight was sufficient to induce complete abortion in mice. Form II rTCS had a propeptide of 19 aa at the C-terminus and was five times less active than Form I in inhibiting protein synthesis by a rabbit reticulocyte lysate." @default.
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• W1990806032 date "2009-01-12" @default.
• W1990806032 modified "2023-09-23" @default.
• W1990806032 title "High level synthesis of biologically active recombinant trichosanthin in Escherichia coli" @default.
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• W1990806032 doi "https://doi.org/10.1111/j.1399-3011.1992.tb01558.x" @default.
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