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• W1990872737 abstract "Abstract The stem rust resistance gene Sr31 , transferred from rye ( Secale cereale ) into wheat ( Triticum aestivum L.) imparts resistance to all the virulent pathotypes of stem rust ( Puccinia graminis f. sp. tritici ) found in India. Wheat genotypes including carriers and non‐carriers of the Sr31 gene were analysed using arbitrary primed polymerase chain reaction (AP‐PCR). AP‐PCR markers viz. SS30.2 580(H) associated with the Sr31 gene and SS26.1 1100 associated with the allele for susceptibility were identified. Linkage between the markers and phenotypes was confirmed by analysing an F 2 population obtained from a cross between a resistant and a susceptible genotype. The markers were tightly linked to the respective alleles. Both the AP‐PCR markers were converted into sequence characterized amplified region (SCAR) markers, viz. SCSS30.2 576 and SCSS26.1 1100 respectively. The markers were validated in two more segregating populations and 49 wheat genotypes. Using both markers it was possible to distinguish the homozygous from the heterozygous carriers of the Sr31 gene in the F 2 generation. The markers developed in this study can be used for pyramiding of the Sr31 gene with other rust resistance genes and in marker‐assisted selection." @default.
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• W1990872737 date "2006-11-17" @default.
• W1990872737 modified "2023-10-10" @default.
• W1990872737 title "Development of SCAR markers for identification of stem rust resistance gene Sr31 in the homozygous or heterozygous condition in bread wheat" @default.
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• W1990872737 doi "https://doi.org/10.1111/j.1439-0523.2006.01282.x" @default.
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