FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990880275> ?p ?o ?g. }

• W1990880275 abstract "The trefoil factor family 2 (TFF2), a member of the trefoil factor family, plays a critical role in the defense and repair of gastrointestinal mucosa. However, its widespread application is hampered by difficulties in large-scale production of the recombinant protein suitable for clinical use. The aim of the present study was to produce hTFF2 by Escherichia coli expression system and explore its in vitro pharmacological characterization. hTFF2 gene encoding mature peptide was obtained by RT-PCR, and then inserted into the expression vector pET32a to construct the recombinant pET32a-hTFF2. After confirmation by gene sequencing, pET32a-hTFF2 was transformed into E. coli Origami B(DE3), and TrxA-hTFF2 fusion protein was expressed by conventional IPTG induction in a shake flask and analyzed with SDS-PAGE and Western-blot. Subsequently, TrxA-hTFF2 was isolated by Ni-NTA affinity chromatography, and ultrafiltration. Finally, we tested the effect of hTFF2 on cell migration in an in vitro restitution model and cell proliferation by MTT assay. The data revealed that the recombinant vector pET32a-hTFF2 was constructed successfully. TrxA-hTFF2 fusion protein was expressed to 246.5mg/L and its purity was above 95% after purification. SDS-PAGE and Western blot analyses showed that the fusion protein presented as a single band with a molecular weight of 32kDa. In vitro model of wounding demonstrated that hTFF2 enhanced migration activity by three folds. MTT assay exhibited a statistically significant dose-dependent growth-enhanced effect. Collectively, the results suggest that the recombinant hTFF2 was expressed in E. coli with high production, purity and biological activity." @default.
• W1990880275 created "2016-06-24" @default.
• W1990880275 creator A5009777873 @default.
• W1990880275 creator A5020588035 @default.
• W1990880275 creator A5032820759 @default.
• W1990880275 creator A5048040922 @default.
• W1990880275 creator A5048225768 @default.
• W1990880275 creator A5050235710 @default.
• W1990880275 creator A5069753768 @default.
• W1990880275 creator A5070931010 @default.
• W1990880275 creator A5087055249 @default.
• W1990880275 date "2010-05-01" @default.
• W1990880275 modified "2023-09-27" @default.
• W1990880275 title "Overexpression of hTFF2 in the pET system and its in vitro pharmacological characterization" @default.
• W1990880275 cites W1826563865 @default.
• W1990880275 cites W1972958208 @default.
• W1990880275 cites W1976162686 @default.
• W1990880275 cites W1985292219 @default.
• W1990880275 cites W1987534021 @default.
• W1990880275 cites W1999738788 @default.
• W1990880275 cites W2017761896 @default.
• W1990880275 cites W2026594771 @default.
• W1990880275 cites W2036224430 @default.
• W1990880275 cites W2039255135 @default.
• W1990880275 cites W2045828366 @default.
• W1990880275 cites W2055646755 @default.
• W1990880275 cites W2091145180 @default.
• W1990880275 cites W2113314122 @default.
• W1990880275 cites W2121889529 @default.
• W1990880275 cites W2135169290 @default.
• W1990880275 cites W2156366212 @default.
• W1990880275 cites W2164901517 @default.
• W1990880275 cites W2167786199 @default.
• W1990880275 cites W2397041308 @default.
• W1990880275 cites W2413180644 @default.
• W1990880275 cites W2142705825 @default.
• W1990880275 doi "https://doi.org/10.1016/j.biopha.2009.12.002" @default.
• W1990880275 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20074900" @default.
• W1990880275 hasPublicationYear "2010" @default.
• W1990880275 type Work @default.
• W1990880275 sameAs 1990880275 @default.
• W1990880275 citedByCount "2" @default.
• W1990880275 countsByYear W19908802752013 @default.
• W1990880275 countsByYear W19908802752017 @default.
• W1990880275 crossrefType "journal-article" @default.
• W1990880275 hasAuthorship W1990880275A5009777873 @default.
• W1990880275 hasAuthorship W1990880275A5020588035 @default.
• W1990880275 hasAuthorship W1990880275A5032820759 @default.
• W1990880275 hasAuthorship W1990880275A5048040922 @default.
• W1990880275 hasAuthorship W1990880275A5048225768 @default.
• W1990880275 hasAuthorship W1990880275A5050235710 @default.
• W1990880275 hasAuthorship W1990880275A5069753768 @default.
• W1990880275 hasAuthorship W1990880275A5070931010 @default.
• W1990880275 hasAuthorship W1990880275A5087055249 @default.
• W1990880275 hasConcept C104317684 @default.
• W1990880275 hasConcept C123894998 @default.
• W1990880275 hasConcept C153911025 @default.
• W1990880275 hasConcept C170344550 @default.
• W1990880275 hasConcept C185592680 @default.
• W1990880275 hasConcept C202751555 @default.
• W1990880275 hasConcept C2776415932 @default.
• W1990880275 hasConcept C2780783641 @default.
• W1990880275 hasConcept C40767141 @default.
• W1990880275 hasConcept C547475151 @default.
• W1990880275 hasConcept C55493867 @default.
• W1990880275 hasConcept C86803240 @default.
• W1990880275 hasConceptScore W1990880275C104317684 @default.
• W1990880275 hasConceptScore W1990880275C123894998 @default.
• W1990880275 hasConceptScore W1990880275C153911025 @default.
• W1990880275 hasConceptScore W1990880275C170344550 @default.
• W1990880275 hasConceptScore W1990880275C185592680 @default.
• W1990880275 hasConceptScore W1990880275C202751555 @default.
• W1990880275 hasConceptScore W1990880275C2776415932 @default.
• W1990880275 hasConceptScore W1990880275C2780783641 @default.
• W1990880275 hasConceptScore W1990880275C40767141 @default.
• W1990880275 hasConceptScore W1990880275C547475151 @default.
• W1990880275 hasConceptScore W1990880275C55493867 @default.
• W1990880275 hasConceptScore W1990880275C86803240 @default.
• W1990880275 hasLocation W19908802751 @default.
• W1990880275 hasLocation W19908802752 @default.
• W1990880275 hasOpenAccess W1990880275 @default.
• W1990880275 hasPrimaryLocation W19908802751 @default.
• W1990880275 hasRelatedWork W2071391374 @default.
• W1990880275 hasRelatedWork W2349517503 @default.
• W1990880275 hasRelatedWork W2352045827 @default.
• W1990880275 hasRelatedWork W2357419405 @default.
• W1990880275 hasRelatedWork W2362236774 @default.
• W1990880275 hasRelatedWork W2367044468 @default.
• W1990880275 hasRelatedWork W2368701960 @default.
• W1990880275 hasRelatedWork W2370414586 @default.
• W1990880275 hasRelatedWork W2373431142 @default.
• W1990880275 hasRelatedWork W2373587422 @default.
• W1990880275 hasRelatedWork W2379488569 @default.
• W1990880275 hasRelatedWork W2381543515 @default.
• W1990880275 hasRelatedWork W2381958483 @default.
• W1990880275 hasRelatedWork W2386275730 @default.
• W1990880275 hasRelatedWork W2388094403 @default.
• W1990880275 hasRelatedWork W2397030661 @default.
• W1990880275 hasRelatedWork W2461283565 @default.
• W1990880275 hasRelatedWork W2889912365 @default.

• next