FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990900376> ?p ?o ?g. }

• W1990900376 endingPage "13032" @default.
• W1990900376 startingPage "13027" @default.
• W1990900376 abstract "The Saccharomyces cerevisiae Rlp7 protein has extensive identity and similarity to the large ribosomal subunit L7 proteins and shares an RNA-binding domain with them. Rlp7p is not a ribosomal protein; however, it is encoded by an essential gene and therefore must perform a function essential for cell growth. In this report, we show that Rlp7p is a nucleolar protein that plays a critical role in processing of precursors to the large ribosomal subunit RNAs. Pulse-chase labeling experiments with Rlp7p-depleted cells reveal that neither 5.8S(S), 5.8S(L), nor 25S is produced, indicating that both the major and minor processing pathways are affected. Analysis of processing intermediates by primer extension indicates that Rlp7p-depleted cells accumulate the 27SA(3) precursor RNA, which is normally the major substrate (85%) used to produce the 5.8S and 25S rRNAs, and the ratio of 27SB(L) to 27SB(S) precursors changes from approximately 1:8 to 8:1 (depleted cells). Because 27SA(3) is the direct precursor to 27SB(S), we conclude that Rlp7p is specifically required for the 5' to 3' exonucleolytic trimming of the 27SA(3) into the 27SB(S) precursor. As it is essential for processing in both the major and minor pathways, we propose that Rlp7p may act as a specificity factor that binds precursor rRNAs and tethers the enzymes that carry out the early 5' to 3' exonucleolytic reactions that generate the mature rRNAs. Rlp7p may also be required for the endonucleolytic cleavage in internal transcribed spacer 2 that separates the 5.8S rRNA from the 25S rRNA." @default.
• W1990900376 created "2016-06-24" @default.
• W1990900376 creator A5012984735 @default.
• W1990900376 creator A5014028838 @default.
• W1990900376 creator A5042800497 @default.
• W1990900376 creator A5081082314 @default.
• W1990900376 date "2000-11-21" @default.
• W1990900376 modified "2023-09-23" @default.
• W1990900376 title "A nucleolar protein related to ribosomal protein L7 is required for an early step in large ribosomal subunit biogenesis" @default.
• W1990900376 cites W1559808711 @default.
• W1990900376 cites W1585970141 @default.
• W1990900376 cites W1599868278 @default.
• W1990900376 cites W1727514194 @default.
• W1990900376 cites W1968109302 @default.
• W1990900376 cites W1968676663 @default.
• W1990900376 cites W1978683810 @default.
• W1990900376 cites W1986813053 @default.
• W1990900376 cites W2015507510 @default.
• W1990900376 cites W2017113017 @default.
• W1990900376 cites W2036403003 @default.
• W1990900376 cites W2044386270 @default.
• W1990900376 cites W2049883153 @default.
• W1990900376 cites W2055827717 @default.
• W1990900376 cites W2073367697 @default.
• W1990900376 cites W2080408951 @default.
• W1990900376 cites W2097654784 @default.
• W1990900376 cites W2097666362 @default.
• W1990900376 cites W2101397045 @default.
• W1990900376 cites W2110544609 @default.
• W1990900376 cites W2111342101 @default.
• W1990900376 cites W2120959840 @default.
• W1990900376 cites W2126219914 @default.
• W1990900376 cites W2141975228 @default.
• W1990900376 cites W2142985191 @default.
• W1990900376 cites W2163636525 @default.
• W1990900376 cites W2165093821 @default.
• W1990900376 cites W21943246 @default.
• W1990900376 doi "https://doi.org/10.1073/pnas.97.24.13027" @default.
• W1990900376 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/27172" @default.
• W1990900376 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11087857" @default.
• W1990900376 hasPublicationYear "2000" @default.
• W1990900376 type Work @default.
• W1990900376 sameAs 1990900376 @default.
• W1990900376 citedByCount "56" @default.
• W1990900376 countsByYear W19909003762012 @default.
• W1990900376 countsByYear W19909003762013 @default.
• W1990900376 countsByYear W19909003762014 @default.
• W1990900376 countsByYear W19909003762015 @default.
• W1990900376 countsByYear W19909003762016 @default.
• W1990900376 countsByYear W19909003762017 @default.
• W1990900376 countsByYear W19909003762018 @default.
• W1990900376 countsByYear W19909003762019 @default.
• W1990900376 countsByYear W19909003762022 @default.
• W1990900376 crossrefType "journal-article" @default.
• W1990900376 hasAuthorship W1990900376A5012984735 @default.
• W1990900376 hasAuthorship W1990900376A5014028838 @default.
• W1990900376 hasAuthorship W1990900376A5042800497 @default.
• W1990900376 hasAuthorship W1990900376A5081082314 @default.
• W1990900376 hasBestOaLocation W19909003762 @default.
• W1990900376 hasConcept C104292427 @default.
• W1990900376 hasConcept C104317684 @default.
• W1990900376 hasConcept C112755997 @default.
• W1990900376 hasConcept C131934819 @default.
• W1990900376 hasConcept C153911025 @default.
• W1990900376 hasConcept C183873130 @default.
• W1990900376 hasConcept C190062978 @default.
• W1990900376 hasConcept C2779419633 @default.
• W1990900376 hasConcept C301363 @default.
• W1990900376 hasConcept C38062823 @default.
• W1990900376 hasConcept C55493867 @default.
• W1990900376 hasConcept C67705224 @default.
• W1990900376 hasConcept C67905577 @default.
• W1990900376 hasConcept C86803240 @default.
• W1990900376 hasConcept C88478588 @default.
• W1990900376 hasConcept C95444343 @default.
• W1990900376 hasConcept C98098607 @default.
• W1990900376 hasConceptScore W1990900376C104292427 @default.
• W1990900376 hasConceptScore W1990900376C104317684 @default.
• W1990900376 hasConceptScore W1990900376C112755997 @default.
• W1990900376 hasConceptScore W1990900376C131934819 @default.
• W1990900376 hasConceptScore W1990900376C153911025 @default.
• W1990900376 hasConceptScore W1990900376C183873130 @default.
• W1990900376 hasConceptScore W1990900376C190062978 @default.
• W1990900376 hasConceptScore W1990900376C2779419633 @default.
• W1990900376 hasConceptScore W1990900376C301363 @default.
• W1990900376 hasConceptScore W1990900376C38062823 @default.
• W1990900376 hasConceptScore W1990900376C55493867 @default.
• W1990900376 hasConceptScore W1990900376C67705224 @default.
• W1990900376 hasConceptScore W1990900376C67905577 @default.
• W1990900376 hasConceptScore W1990900376C86803240 @default.
• W1990900376 hasConceptScore W1990900376C88478588 @default.
• W1990900376 hasConceptScore W1990900376C95444343 @default.
• W1990900376 hasConceptScore W1990900376C98098607 @default.
• W1990900376 hasIssue "24" @default.
• W1990900376 hasLocation W19909003761 @default.
• W1990900376 hasLocation W19909003762 @default.
• W1990900376 hasLocation W19909003763 @default.
• W1990900376 hasLocation W19909003764 @default.

• next