FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1990900446> ?p ?o ?g. }

• W1990900446 endingPage "135" @default.
• W1990900446 startingPage "124" @default.
• W1990900446 abstract "Umbilical cord blood (CB) CD34(+) cells, on the basis of flow cytometry analysis, are comprised of multiple populations. In in vitro assays, only CD34(regular) FSC(high) cells are functional and low percentages of nonfunctional CD34(regular) FSC(low) cells were determined to be present in liquid-stored CB. Liquid-stored CD34(regular) FSC(high) cells prior to cryopreservation were judged to be functional by the formation of erythroid and myeloid colonies and transmigration assays. We have further evaluated the occurrence of apoptosis in CB CD34(+) cells using various apoptotic markers to understand better the influence of storage conditions that could be utilized with transplantation of CB. Of the CD34(regular) FSC(low) cells shown in the present study, 20-45% were labeled with the apoptotic reagents annexin-V, fluorescent caspase peptide substrates, and the anti-mitochondrial antibody APO2.7, but these cells were minimally stained with 7-aminoactinomycin-D (7-AAD). These apoptotic reagents identify different cellular targets, indicating the initiation of the apoptotic cascade prior to cryopreservation/thawing. Following cryopreservation and thawing, the apoptotic markers SYTO-16, tetramethyl rhodamine ethyl ester (TMRE), and 7-AAD showed the presence of apoptotic cells. After cryopreservation/thawing, enumeration of CB CD34(+) cells was reduced 10-65% when excluding cells positive for apoptotic markers. We attempted to limit the progression of apoptosis observed after cryopreservation/thawing by the addition of anti-apoptotic reagents z-VAD-fmk (100 microM) and Q-VD-OPH (100 microM) (peptide inhibitors of caspases) without or with the inclusion of survival reagents for CD34(+) cells-stromal-derived factor-1 (SDF-1), stem cell factor (SCF), thrombopoietin, and diprotin A, an inhibitor of CD26 prior to cryopreservation. The expression of apoptosis markers was minimally affected even when using combinations of caspase inhibitors/ CD34(+) cell survival cytokines in an attempt to block apoptosis caused by cryopreservation/thawing. Decreases in apoptosis marker reactivity following cryopreservation were not observed except for a reduced expression of APO2.7 reactivity with z-VAD-fmk and Q-VD-OPH caspase inhibitors. The ability of the inhibitors of apoptosis of CD34(+) cells to generate CFU-GM, CFU-MK, or BFUE colonies was also unaffected except with z-VAD-fmk (100 microM) and Q-VD-OPH (100 microM). The occurrence of apoptosis, as measured by flow cytometry with selected apoptotic markers, suggests a reduction in the number of viable CD34(+) cells." @default.
• W1990900446 created "2016-06-24" @default.
• W1990900446 creator A5001679305 @default.
• W1990900446 creator A5003667141 @default.
• W1990900446 creator A5042766411 @default.
• W1990900446 creator A5051164101 @default.
• W1990900446 creator A5056770133 @default.
• W1990900446 date "2006-02-01" @default.
• W1990900446 modified "2023-09-27" @default.
• W1990900446 title "Evaluation of the Reactivity of Apoptosis Markers Before and After Cryopreservation in Cord Blood CD34⁺Cells" @default.
• W1990900446 cites W1964281995 @default.
• W1990900446 cites W1974075917 @default.
• W1990900446 cites W1981585635 @default.
• W1990900446 cites W1982990241 @default.
• W1990900446 cites W1984425795 @default.
• W1990900446 cites W1989904308 @default.
• W1990900446 cites W1991917280 @default.
• W1990900446 cites W1994598048 @default.
• W1990900446 cites W1994700879 @default.
• W1990900446 cites W1999451125 @default.
• W1990900446 cites W2010055592 @default.
• W1990900446 cites W2012295912 @default.
• W1990900446 cites W2019438578 @default.
• W1990900446 cites W2022942708 @default.
• W1990900446 cites W2029108116 @default.
• W1990900446 cites W2032481185 @default.
• W1990900446 cites W2058108603 @default.
• W1990900446 cites W2077255444 @default.
• W1990900446 cites W2077566755 @default.
• W1990900446 cites W2080682839 @default.
• W1990900446 cites W2085820900 @default.
• W1990900446 cites W2090956270 @default.
• W1990900446 cites W2125017552 @default.
• W1990900446 cites W2140654809 @default.
• W1990900446 cites W2143096378 @default.
• W1990900446 cites W2153305674 @default.
• W1990900446 cites W2153532735 @default.
• W1990900446 cites W2156880644 @default.
• W1990900446 cites W2164696469 @default.
• W1990900446 cites W4234338318 @default.
• W1990900446 cites W4243388207 @default.
• W1990900446 cites W4321429393 @default.
• W1990900446 cites W4375906095 @default.
• W1990900446 doi "https://doi.org/10.1089/scd.2006.15.124" @default.
• W1990900446 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16522170" @default.
• W1990900446 hasPublicationYear "2006" @default.
• W1990900446 type Work @default.
• W1990900446 sameAs 1990900446 @default.
• W1990900446 citedByCount "22" @default.
• W1990900446 countsByYear W19909004462012 @default.
• W1990900446 countsByYear W19909004462014 @default.
• W1990900446 countsByYear W19909004462015 @default.
• W1990900446 countsByYear W19909004462016 @default.
• W1990900446 countsByYear W19909004462017 @default.
• W1990900446 countsByYear W19909004462018 @default.
• W1990900446 countsByYear W19909004462019 @default.
• W1990900446 crossrefType "journal-article" @default.
• W1990900446 hasAuthorship W1990900446A5001679305 @default.
• W1990900446 hasAuthorship W1990900446A5003667141 @default.
• W1990900446 hasAuthorship W1990900446A5042766411 @default.
• W1990900446 hasAuthorship W1990900446A5051164101 @default.
• W1990900446 hasAuthorship W1990900446A5056770133 @default.
• W1990900446 hasConcept C10205521 @default.
• W1990900446 hasConcept C153911025 @default.
• W1990900446 hasConcept C16685009 @default.
• W1990900446 hasConcept C179933525 @default.
• W1990900446 hasConcept C190283241 @default.
• W1990900446 hasConcept C196843134 @default.
• W1990900446 hasConcept C203014093 @default.
• W1990900446 hasConcept C2780914630 @default.
• W1990900446 hasConcept C28328180 @default.
• W1990900446 hasConcept C553184892 @default.
• W1990900446 hasConcept C55493867 @default.
• W1990900446 hasConcept C71924100 @default.
• W1990900446 hasConcept C86803240 @default.
• W1990900446 hasConcept C88634738 @default.
• W1990900446 hasConcept C95444343 @default.
• W1990900446 hasConceptScore W1990900446C10205521 @default.
• W1990900446 hasConceptScore W1990900446C153911025 @default.
• W1990900446 hasConceptScore W1990900446C16685009 @default.
• W1990900446 hasConceptScore W1990900446C179933525 @default.
• W1990900446 hasConceptScore W1990900446C190283241 @default.
• W1990900446 hasConceptScore W1990900446C196843134 @default.
• W1990900446 hasConceptScore W1990900446C203014093 @default.
• W1990900446 hasConceptScore W1990900446C2780914630 @default.
• W1990900446 hasConceptScore W1990900446C28328180 @default.
• W1990900446 hasConceptScore W1990900446C553184892 @default.
• W1990900446 hasConceptScore W1990900446C55493867 @default.
• W1990900446 hasConceptScore W1990900446C71924100 @default.
• W1990900446 hasConceptScore W1990900446C86803240 @default.
• W1990900446 hasConceptScore W1990900446C88634738 @default.
• W1990900446 hasConceptScore W1990900446C95444343 @default.
• W1990900446 hasIssue "1" @default.
• W1990900446 hasLocation W19909004461 @default.
• W1990900446 hasLocation W19909004462 @default.
• W1990900446 hasOpenAccess W1990900446 @default.
• W1990900446 hasPrimaryLocation W19909004461 @default.
• W1990900446 hasRelatedWork W1972464992 @default.

• next