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- W1990907698 abstract "A highly specific and sensitive automated high-performance liquid chromatographic method for the simultaneous determination of catecholamines (CAs; norepinephrine, epinephrine, and dopamine) and their 3-O-methyl metabolites (normetanephrine, metanephrine, and 3-methoxytyramine) is described. Automated precolumn ion-exchange extraction of diluted plasma is coupled with HPLC separation of CAs and their 3-O-methyl metabolites on an ODS column, postcolumn coulometric oxidation, fluorescence derivatization with ethylenediamine, and finally peroxyoxalate chemiluminescence reaction detection. The detection limits were about 3 fmol for norepinephrine, epinephrine, and dopamine, 5 fmol for normetanephrine, and 10 fmol for metanephrine and 3-methoxytyramine (signal-to-noise ratio of 3). Fifty microliters of rat plasma was used and 4-methoxytyramine was employed as an internal standard. The relative standard deviations for the method (n = 5) were 2.5-7.6% for the intraday assay and 6.3-9.1% for the interday assay. The method was applicable to the determination of normetanephrine and metanephrine in 50 microl of rat plasma." @default.
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- W1990907698 date "1999-05-01" @default.
- W1990907698 modified "2023-10-06" @default.
- W1990907698 title "Simultaneous Automatic Determination of Catecholamines and Their 3-O-Methyl Metabolites in Rat Plasma by High-Performance Liquid Chromatography Using Peroxyoxalate Chemiluminescence Reaction" @default.
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- W1990907698 doi "https://doi.org/10.1006/abio.1999.4043" @default.
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