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- W1990980287 abstract "In the past years, research on the flagellate unicellular alga Chlamydomonas reinhardtii has entered a new era based on the availability of its complete genome. Since this green alga can be grown relatively easy in a short time-range, sufficient biological material is available to efficiently establish biochemical purification procedures of sub-cellular fractions. Combined with the available genome sequences, this paved the way to perform analysis of specific sub-proteomes by mass spectrometry. In this review, several approaches that provided comprehensive lists of components of certain sub-cellular compartments and their biological relevance will be described. These include proteins of chloroplast ribosomes, of flagella, of the eyespot as well as posttranslational and environmentally modified sub-proteomes. The power of such proteome approaches lies in the identification of novel components and modifications of a given sub-proteome that have not been discovered before. Information is usually gained at a large scale and is very valuable to further understand biological processes of a given cellular sub-compartment. But clearly the arduous task has then to be performed to further analyze the function of specific proteins/genes by RNA interference technology, mutant analyses or methods for identifying the protein interaction network within a sub-proteome." @default.
- W1990980287 created "2016-06-24" @default.
- W1990980287 creator A5022468377 @default.
- W1990980287 creator A5023567333 @default.
- W1990980287 creator A5090276977 @default.
- W1990980287 date "2009-02-01" @default.
- W1990980287 modified "2023-10-17" @default.
- W1990980287 title "Sub‐proteome analysis in the green flagellate alga <i>Chlamydomonas reinhardtii</i>" @default.
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- W1990980287 doi "https://doi.org/10.1002/jobm.200800292" @default.
- W1990980287 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19253330" @default.
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