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- W1991005968 abstract "The principle of the double isotope technique for measuring iron absorption is to inject one isotope, 59Fe, intravenously and give the other, 55Fe, orally at the same time (Saylor and Finch, 1953). The 59Fe is used to determine the percentage of plasma iron that is used for haemoglobin synthesis and it is assumed that the percentage utilization of the absorbed 55Fe will be the same. From the relative amounts of the two isotopes present in the red cells at 10 days, it is possible to calculate the percentage of the oral 55Fe absorbed. For the counting of the two isotopes in blood samples, the iron must be separated from the blood by digestion and precipitation followed by electroplating onto metal planchettes. The technique as originally described and as used by subsequent workers had a number of disadvantages. The various types of electroplating apparatus have proved unreliable in use or are complex in construction and the calculations have depended on the accurate recovery of known amounts of haemoglobin iron from blood samples (Hallberg and Brise, 1960; Brise and Hallberg, 1962). This is not easy to achieve in practice. The counting methods devised, including the use of gas flow counters and filters, have in our experience variable efficiencies. The present report describes a relatively simple and reliable technique which has proved suitable for both single and serial measurements of iron absorption. The normal range of absorption by this method has been defined using a group of control subjects carefully screened to exclude mild unsuspected iron deficiency. Also reported is a comparison of the results obtained by this technique in a number of patients with widely differing absorption rates in whom iron absorption was also determined simultaneously by stool counting and by whole body counting." @default.
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- W1991005968 date "1965-11-01" @default.
- W1991005968 modified "2023-09-27" @default.
- W1991005968 title "The Measurement of Iron Absorption by the Double Isotope Technique" @default.
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- W1991005968 doi "https://doi.org/10.1111/j.1365-2141.1965.tb00111.x" @default.
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