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• W1991035833 abstract "Abstract The in vitro differentiation of embryonic stem cells into glia has received relatively limited attention to date when compared with the interest in the generation of neurons. We are interested in a particular glial phenotype, the aldynoglia, and their differentiation from multipotential neural precursors (MNP), since this type of glia can promote neuronal regeneration. We constructed cDNA libraries from cultures of purified olfactory ensheathing cells (OEC), an aldynoglia cell type, and MNP to perform subtractive hybridization. As a result, we isolated four genes from the OEC: one tenascin C (Tn‐C) isoform, Insulin‐like growth factor binding protein 5 (Igfbp‐5), cytochrome oxidase subunit I (COX1) and a phosphodiesterase for cyclic nucleotides (CNPase). With the exception of CNPase, these genes are expressed more strongly in the OEC than in the MNP and moreover, the expression of all four is induced when MNP were exposed to OEC conditioned media. The data suggest a role for these genes in MNP differentiation, and their products appear to represent characteristic proteins of the aldynoglia phenotype." @default.
• W1991035833 created "2016-06-24" @default.
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• W1991035833 date "2009-09-20" @default.
• W1991035833 modified "2023-10-18" @default.
• W1991035833 title "Subtractive hybridization identifies genes differentially expressed by olfactory ensheathing cells and neural stem cells" @default.
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• W1991035833 doi "https://doi.org/10.1016/j.ijdevneu.2009.08.019" @default.
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