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- W1991075508 abstract "Abstract 1. The effect of the illumination of whole plants of Pisum sativum immediately prior to chloroplast isolation on subsequent photophosphorylation in vitro was investigated. Using a new technique, chloroplasts were isolated within 2 min of harvesting the plants. 2. Chloroplasts isolated from illuminated pea plants had a rate of endogenous photophosphorylation of 24 μmoles ATP×(mg chlorophyll) −1 ×h −1 , while those from plants in the dark had a rate of 12. Changes between the two levels had half-times of about 5 min. The addition of ferricyanide and N -methylphenazonium methosulfate increased the photophosphorylation rate about twice as much with chloroplasts isolated from plants in the light compared to those isolated from plants in the dark. An action spectrum indicated that light absorbed by chloroplasts in vivo was responsible for the increased photophosphorylation in vitro . 3. Chloroplasts isolated from illuminated plants had a volume approx. 15% less than those from plants in the dark. The reversal in vitro of this shrinkage and of the elevated photophosphorylation rate both had half-times of about 10 min. Light-induced chloroplast shrinkage in vivo is discussed in connection with the increased efficiency of photophosphorylation in vitro for chloroplasts isolated from illuminated plants." @default.
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- W1991075508 date "1968-01-01" @default.
- W1991075508 modified "2023-09-26" @default.
- W1991075508 title "Chloroplast shrinkage and increased photophosphorylation in vitro upon illuminating intact plants of Pisum sativum" @default.
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- W1991075508 doi "https://doi.org/10.1016/0005-2728(68)90157-6" @default.
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