FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991130438> ?p ?o ?g. }

• W1991130438 endingPage "42" @default.
• W1991130438 startingPage "24" @default.
• W1991130438 abstract "Embryo transfer is a reproductive technique that has a major impact on the dissemination of economically important genes and the rate of genetic gain in breeding schemes. In recent years, there has been increasing interest in the use of sexed and genotyped embryos in commercial embryo transfer programs. Marker/gene assisted selection (MAS/GAS) projects can be performed in the pre-implantation stage through mass production of characterized embryos. Biopsy of a few cells in the morulla stage is essential for pre-implantation genetic diagnosis (PGD), in which sex determination, evaluation of disease genes, and genotyping for candidate genes are performed. Limited quantity of cells and low amount of DNA restrict the use of multiple molecular analyses in PGD programs. Recently, whole genome amplification (WGA) techniques promise to overcome this problem by providing sufficient input DNA for analysis. Among several techniques proposed for WGA, the primer extension pre-amplification (PEP) and the improved-primer extension pre-amplification (I-PEP) methods are the most commonly used. However, these methods are time-consuming and need more than 12 h amplification cycles. Since the time is a critical parameter in the successful characterized embryo transfer, the shortening of diagnosis time is highly desirable. In this study, we developed a short and simple I-PEP procedure (~3 h) and evaluated its performance for the amplification of bovine genomic DNA. We assessed short WGA procedure by polymerase chain reaction (PCR) amplification of 7 specific loci. The results indicated that the short procedure possesses enough sensitivity for the molecular genetic analysis of 1 input cell. Although the efficiency of the method was 100%, there was an inconsistency between genomic DNA (gDNA) and whole genome amplification product (wgaDNA) genotypes for kappa-casein locus; that is, however, most likely due to allele drop-out (ADO) or false homozigocity. The results of this study indicate that with the application of reliable methods, WGA-amplified bovine DNA will be a useful source for sexing and genotyping bovine embryos in several quantitative trait locus (QTL) markers." @default.
• W1991130438 created "2016-06-24" @default.
• W1991130438 creator A5018197642 @default.
• W1991130438 creator A5022626935 @default.
• W1991130438 creator A5026938429 @default.
• W1991130438 creator A5071626227 @default.
• W1991130438 date "2012-01-01" @default.
• W1991130438 modified "2023-09-25" @default.
• W1991130438 title "A Short and Simple Improved-Primer Extension Preamplification (I-PEP) Procedure for Whole Genome Amplification (WGA) of Bovine Cells" @default.
• W1991130438 cites W1577804299 @default.
• W1991130438 cites W1584451756 @default.
• W1991130438 cites W1859302788 @default.
• W1991130438 cites W1965802554 @default.
• W1991130438 cites W1968762427 @default.
• W1991130438 cites W1972305983 @default.
• W1991130438 cites W1974078605 @default.
• W1991130438 cites W1980447644 @default.
• W1991130438 cites W1982337485 @default.
• W1991130438 cites W1984731778 @default.
• W1991130438 cites W1996811680 @default.
• W1991130438 cites W1998596397 @default.
• W1991130438 cites W2006926214 @default.
• W1991130438 cites W2018111848 @default.
• W1991130438 cites W2033186236 @default.
• W1991130438 cites W2035038219 @default.
• W1991130438 cites W2036566970 @default.
• W1991130438 cites W2038752939 @default.
• W1991130438 cites W2041423588 @default.
• W1991130438 cites W2063513406 @default.
• W1991130438 cites W2063775230 @default.
• W1991130438 cites W2066175709 @default.
• W1991130438 cites W2072027611 @default.
• W1991130438 cites W2076669821 @default.
• W1991130438 cites W2086112478 @default.
• W1991130438 cites W2091167042 @default.
• W1991130438 cites W2093632538 @default.
• W1991130438 cites W2097331522 @default.
• W1991130438 cites W2097348296 @default.
• W1991130438 cites W2106336173 @default.
• W1991130438 cites W2118094114 @default.
• W1991130438 cites W2120725135 @default.
• W1991130438 cites W2121628365 @default.
• W1991130438 cites W2124976462 @default.
• W1991130438 cites W2125637629 @default.
• W1991130438 cites W2134373876 @default.
• W1991130438 cites W2142603715 @default.
• W1991130438 cites W2145903120 @default.
• W1991130438 cites W2151389923 @default.
• W1991130438 cites W2160509449 @default.
• W1991130438 cites W2163175450 @default.
• W1991130438 cites W2164859157 @default.
• W1991130438 cites W2169241047 @default.
• W1991130438 doi "https://doi.org/10.1080/10495398.2011.630907" @default.
• W1991130438 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22292699" @default.
• W1991130438 hasPublicationYear "2012" @default.
• W1991130438 type Work @default.
• W1991130438 sameAs 1991130438 @default.
• W1991130438 citedByCount "7" @default.
• W1991130438 countsByYear W19911304382012 @default.
• W1991130438 countsByYear W19911304382013 @default.
• W1991130438 countsByYear W19911304382014 @default.
• W1991130438 countsByYear W19911304382015 @default.
• W1991130438 countsByYear W19911304382022 @default.
• W1991130438 countsByYear W19911304382023 @default.
• W1991130438 crossrefType "journal-article" @default.
• W1991130438 hasAuthorship W1991130438A5018197642 @default.
• W1991130438 hasAuthorship W1991130438A5022626935 @default.
• W1991130438 hasAuthorship W1991130438A5026938429 @default.
• W1991130438 hasAuthorship W1991130438A5071626227 @default.
• W1991130438 hasConcept C104317684 @default.
• W1991130438 hasConcept C105580179 @default.
• W1991130438 hasConcept C135763542 @default.
• W1991130438 hasConcept C141231307 @default.
• W1991130438 hasConcept C153911025 @default.
• W1991130438 hasConcept C156385826 @default.
• W1991130438 hasConcept C160099875 @default.
• W1991130438 hasConcept C17757408 @default.
• W1991130438 hasConcept C178790620 @default.
• W1991130438 hasConcept C185592680 @default.
• W1991130438 hasConcept C194857396 @default.
• W1991130438 hasConcept C196843134 @default.
• W1991130438 hasConcept C2777563447 @default.
• W1991130438 hasConcept C31467283 @default.
• W1991130438 hasConcept C49105822 @default.
• W1991130438 hasConcept C54355233 @default.
• W1991130438 hasConcept C70721500 @default.
• W1991130438 hasConcept C78063203 @default.
• W1991130438 hasConcept C8185291 @default.
• W1991130438 hasConcept C86803240 @default.
• W1991130438 hasConceptScore W1991130438C104317684 @default.
• W1991130438 hasConceptScore W1991130438C105580179 @default.
• W1991130438 hasConceptScore W1991130438C135763542 @default.
• W1991130438 hasConceptScore W1991130438C141231307 @default.
• W1991130438 hasConceptScore W1991130438C153911025 @default.
• W1991130438 hasConceptScore W1991130438C156385826 @default.
• W1991130438 hasConceptScore W1991130438C160099875 @default.
• W1991130438 hasConceptScore W1991130438C17757408 @default.
• W1991130438 hasConceptScore W1991130438C178790620 @default.

• next