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- W1991140602 abstract "Seedgermination in black cohosh was systematically examined in eighteen populationsincluding 15 USDA accessions with an effective protocol for the consistent,season independent germination of this valuable alternative specialty crop. Two in vitro approaches were investigated for breaking the complex doubledormancy of black cohosh seeds for yearround germination ofplants for increased cultivation and laboratory studies. The first approach wasa two-step alternating temperature stratification in which surfacesterilized seeds were incubated in darkness at 25?C for two weeks followed byincubation at alternating temperatures of 20?C and 8?C for 12-hour periods witha 16-hour photoperiod for 12 months. The second was a three-step-approach thatinvolved initial stratification of seeds in darkness at 25?C for two weeks,followed by incubation at 4?C in darkness for 3 -4 months and thencultivation at 25?C with a 16-hour photoperiod to generateseedlings. Although both approaches broke double dormancy for black cohosh seedgermination, the three-step-stratification technique yielded higher percentageseed germination in less time when compared to the two-step scheme, includingfor seeds stored over two years. Additional factors of critical importance forefficient germination included the selection of healthy and viableseeds, as well as thorough but non-excessive surface sterilization to controlbacterial and fungal contamination. The invitro approach for black cohosh germination allowed year-round cultivationand culture of a number of different genotypic accessions to enable laboratorybased studies on cell culture and transformation approaches to aid indeciphering gene-metabolite relationships in this important medicinal plant." @default.
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- W1991140602 date "2013-01-01" @default.
- W1991140602 modified "2023-09-24" @default.
- W1991140602 title "Efficient, Season-Independent Seed Germination in Black Cohosh (<i>Actaea racemosa</i> L.)" @default.
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- W1991140602 doi "https://doi.org/10.4236/ajps.2013.45a012" @default.
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