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• W1991145526 abstract "The negatively charged nitrogen-vacancy (NV^–) center in bulk diamond is a photostable fluorophore with a radiative lifetime of 11.6 ns at room temperature. The lifetime substantially increases to ~20 ns for diamond nanoparticles (size ~ 100 nm) suspended in water due to the change in refractive index of the surrounding medium of the NV^– centers. This fluorescence decay time is much longer than that (typically 1 − 4 ns) of endogenous and exogenous fluorophores commonly used in biological imaging, making it possible to detect NV^–-containing nanodiamonds <i>in vivo</i> at the single particle level by fluorescence lifetime imaging microscopy (FLIM). We demonstrate the feasibility of this approach using <i>Caenorhabditis elegans</i> (<i>C. elegans</i>) as a model organism." @default.
• W1991145526 created "2016-06-24" @default.
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• W1991145526 date "2013-03-29" @default.
• W1991145526 modified "2023-10-09" @default.
• W1991145526 title "Fluorescence lifetime imaging microscopy of nanodiamonds in vivo" @default.
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• W1991145526 doi "https://doi.org/10.1117/12.2004494" @default.
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