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• W1991148613 abstract "The gas vesicles isolated from the cells of filamentous cyanobacterium Anabaena flos-aquae were treated and sterilized with glutaraldehyde and then evaluated for their effectiveness as gas carriers in cell culture. Anchorage-dependent Vero cells were grown in a packed bed of microcarrier beads under the perfusion of Dulbecco’s Modified Eagle’s Medium with 1% serum. The culture medium supplemented with 1.8% (v/v) gas vesicles was found to support a 30% higher maximum glucose utilization rate than the same medium without gas vesicles. The gas vesicle suspension was confirmed to have no apparent effects on cell metabolism in T-flask cultures. The study results indicated that the gas vesicles, with high oxygen carrying capacity, can be used to increase the oxygen supply in cell culture systems." @default.
• W1991148613 created "2016-06-24" @default.
• W1991148613 creator A5024803902 @default.
• W1991148613 creator A5049436539 @default.
• W1991148613 date "2007-02-20" @default.
• W1991148613 modified "2023-10-16" @default.
• W1991148613 title "Use of cyanobacterial gas vesicles as oxygen carriers in cell culture" @default.
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• W1991148613 doi "https://doi.org/10.1007/s10616-007-9044-9" @default.
• W1991148613 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3449414" @default.
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