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• W1991152743 abstract "Production and analysis of a viral complex derived from a radiation-induced leukemia virus of the C57BL mouse The MuLV isolate RadLV-Rs derived from a radiation-induced leukemia of a C57BL mouse was shown to induce preferentially lymphoreticulum cell neoplasms of spleen and lymph nodes rather than thymomas. Several cell lines were established in culture from spleen or lymph node tumors induced by RaLV-Rs. These lines were originally composed of round shaped virus-producing cells but their ability to produce virus disappeared progressively with serial passages and simultaneously they were growing as a monolayer of polygonal epithelial-like cells. Several clones were derived from one of the tumor cell lines and the presence of round cells correlated positively with the viral release. Besides these morphological differences the karyotype of the clones chronically producing virus was strongly modified. Thus, cells of clone 13-3C (producing a virus called 13C) contained 43 chromosomes, a large number of which were metacentrics or minutes. On the opposite, clone 13-2D which had the same origin as 13–3C but did not produce virus had 70 acrocentric chromosomes. The in vitro produced virus 13C had the same pathogenic effects as the original RaLV-Rs; on the basis of its biochemical and morphological properties it could be classified as a type-C retrovirus and it also induced Gross type cell surface antigens. In contrast to most of the MuLV, 13C virus did not show any helper activity on the MSV integrated in S+L- mouse cells, however syncytia formation was readily observed after cocultivation of 13–3C and XC cells. The 13C virus was shown to be a complex composed of an ecotropic MuLV which preferentially infects B type mouse cells (MuLV-B) and a xenotropic virus (MuLV-X). The MuLV-B had no helper activity for integrated MSV and did not induce XC syncytia formation. The MuLV-X was a potent helper for MSV in S+L- mink cells but did not induce XC syncytia formation." @default.
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• W1991152743 date "1978-07-15" @default.
• W1991152743 modified "2023-10-14" @default.
• W1991152743 title "Production et Analyse D'un Complexe Viral Dérivé D'un Virus des Radioleucoses de la Souris C57BL" @default.
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• W1991152743 doi "https://doi.org/10.1002/ijc.2910220117" @default.
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