FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991152875> ?p ?o ?g. }

• W1991152875 abstract "The plasmid pBR322 was treated with BamHI, PvuII and gamma-irradiation to generate double-strand breaks (dsb) containing differently structured ends. Transformation efficiencies, mutation frequencies and clone analyses of enzymatically damaged DNA are compared with the corresponding results from radiolytically damaged DNA. In E. coli K-12 SFX, the yield of transformants produced by the action of BamHI, PvuII and gamma-irradiation (30 Gy) is 4.3%, 0.14%, and 0.10%, respectively. The survival of open circular DNA (ocDNA) produced by 30 Gy is 1.3%. The transformation efficiencies show only a slight dependence on SOS induction and on the RecA protein. Mutation frequencies to tetracycline sensitivity (tets) per surviving plasmid are 2.6% (BamHI), 11.8% (PvuII) and 0.2% (gamma-irradiated DNA with 30 Gy containing approximately 50% ocDNA and 50% linearized (lin) DNA). The mutation frequency is low at all radiation doses studied (1-50 Gy). Only 15% of the DNA of the tets mutants from gamma-irradiated plasmids contained deletions whereas with enzymatically damaged DNA, 30-50% (BamHI) or 90% (PvuII) contained deletions. In all cases, the deletions comprised 500-1700 base pairs (bp). After SOS induction of the host cells, the mutation frequency of gamma-irradiated plasmids increased by a factor of 4, whereas that of the enzymatically damaged plasmids did not change. For the repair of the enzymatically linearized DNA 2 recombinational pathways are discussed which lead to deletant (pathway I) and non-deletant transformants (pathway II). In addition, BamHI-linearized plasmids may be repaired by enzyme-induced or spontaneous circular alignment followed by ligation. The high percentage of deletions of the tets mutations for PvuII-linearized DNA with blunt ends is explained by the illegitimate or site-specific recombination pathway I (see text). The lower percentage of deletions of the tets mutations with BamHI-linearized DNA with short cohesive ends (4 bp) is proposed to be due to a greater contribution of pathway II and/or by circular alignment followed by ligation. The very small yield and the low percentage of deletant mutations of tets mutants from radiolytically damaged DNA is proposed to be due to the large overlapping ends (16-100 bp) of the linDNA which easily leads to circular alignment followed by excision repair. The repair of radiolytically produced ocDNA is predominantly due to excision repair. In agreement with this interpretation is the observation that SOS induction of the host increases the mutation incidence of radiolytically damaged DNA but not of enzymatically damaged DNA." @default.
• W1991152875 created "2016-06-24" @default.
• W1991152875 creator A5034850641 @default.
• W1991152875 creator A5042336596 @default.
• W1991152875 creator A5065680915 @default.
• W1991152875 date "1988-11-01" @default.
• W1991152875 modified "2023-09-26" @default.
• W1991152875 title "Repair of the plasmid pBR322 damaged by γ-irradiation or by restriction endonucleases using different recombination-proficient E. coli strains" @default.
• W1991152875 cites W1156250817 @default.
• W1991152875 cites W1483306411 @default.
• W1991152875 cites W1542853174 @default.
• W1991152875 cites W1759054411 @default.
• W1991152875 cites W184292948 @default.
• W1991152875 cites W1946095538 @default.
• W1991152875 cites W1973501326 @default.
• W1991152875 cites W1983453657 @default.
• W1991152875 cites W1991513478 @default.
• W1991152875 cites W1997953581 @default.
• W1991152875 cites W2004498595 @default.
• W1991152875 cites W2006955903 @default.
• W1991152875 cites W2013640938 @default.
• W1991152875 cites W2019410656 @default.
• W1991152875 cites W2023132424 @default.
• W1991152875 cites W2042449853 @default.
• W1991152875 cites W2064867925 @default.
• W1991152875 cites W2068748426 @default.
• W1991152875 cites W2071065816 @default.
• W1991152875 cites W2073803742 @default.
• W1991152875 cites W2082805027 @default.
• W1991152875 cites W2084655319 @default.
• W1991152875 cites W2084695608 @default.
• W1991152875 cites W2085526514 @default.
• W1991152875 cites W2091385345 @default.
• W1991152875 cites W2092219738 @default.
• W1991152875 cites W2135865099 @default.
• W1991152875 cites W2149378783 @default.
• W1991152875 cites W2176552396 @default.
• W1991152875 cites W2205208813 @default.
• W1991152875 cites W2320200811 @default.
• W1991152875 cites W4296765338 @default.
• W1991152875 doi "https://doi.org/10.1016/0167-8817(88)90021-1" @default.
• W1991152875 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2847036" @default.
• W1991152875 hasPublicationYear "1988" @default.
• W1991152875 type Work @default.
• W1991152875 sameAs 1991152875 @default.
• W1991152875 citedByCount "12" @default.
• W1991152875 crossrefType "journal-article" @default.
• W1991152875 hasAuthorship W1991152875A5034850641 @default.
• W1991152875 hasAuthorship W1991152875A5042336596 @default.
• W1991152875 hasAuthorship W1991152875A5065680915 @default.
• W1991152875 hasConcept C102306862 @default.
• W1991152875 hasConcept C104317684 @default.
• W1991152875 hasConcept C128319531 @default.
• W1991152875 hasConcept C143065580 @default.
• W1991152875 hasConcept C153911025 @default.
• W1991152875 hasConcept C204241405 @default.
• W1991152875 hasConcept C205225487 @default.
• W1991152875 hasConcept C22744801 @default.
• W1991152875 hasConcept C42174275 @default.
• W1991152875 hasConcept C501734568 @default.
• W1991152875 hasConcept C54355233 @default.
• W1991152875 hasConcept C547475151 @default.
• W1991152875 hasConcept C552990157 @default.
• W1991152875 hasConcept C86803240 @default.
• W1991152875 hasConceptScore W1991152875C102306862 @default.
• W1991152875 hasConceptScore W1991152875C104317684 @default.
• W1991152875 hasConceptScore W1991152875C128319531 @default.
• W1991152875 hasConceptScore W1991152875C143065580 @default.
• W1991152875 hasConceptScore W1991152875C153911025 @default.
• W1991152875 hasConceptScore W1991152875C204241405 @default.
• W1991152875 hasConceptScore W1991152875C205225487 @default.
• W1991152875 hasConceptScore W1991152875C22744801 @default.
• W1991152875 hasConceptScore W1991152875C42174275 @default.
• W1991152875 hasConceptScore W1991152875C501734568 @default.
• W1991152875 hasConceptScore W1991152875C54355233 @default.
• W1991152875 hasConceptScore W1991152875C547475151 @default.
• W1991152875 hasConceptScore W1991152875C552990157 @default.
• W1991152875 hasConceptScore W1991152875C86803240 @default.
• W1991152875 hasLocation W19911528751 @default.
• W1991152875 hasLocation W19911528752 @default.
• W1991152875 hasOpenAccess W1991152875 @default.
• W1991152875 hasPrimaryLocation W19911528751 @default.
• W1991152875 hasRelatedWork W1841638739 @default.
• W1991152875 hasRelatedWork W2020025671 @default.
• W1991152875 hasRelatedWork W2020577684 @default.
• W1991152875 hasRelatedWork W2031800031 @default.
• W1991152875 hasRelatedWork W2038876989 @default.
• W1991152875 hasRelatedWork W2074820260 @default.
• W1991152875 hasRelatedWork W2110037457 @default.
• W1991152875 hasRelatedWork W2359129071 @default.
• W1991152875 hasRelatedWork W2405433527 @default.
• W1991152875 hasRelatedWork W2442348340 @default.
• W1991152875 isParatext "false" @default.
• W1991152875 isRetracted "false" @default.
• W1991152875 magId "1991152875" @default.
• W1991152875 workType "article" @default.