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• W1991159698 endingPage "709" @default.
• W1991159698 startingPage "692" @default.
• W1991159698 abstract "Bordetella pertussis BvgA is a global response regulator that activates virulence genes, including adhesin-encoding fim3 and fhaB. At the fhaB promoter, PfhaB, a BvgA binding site lies immediately upstream of the − 35 promoter element recognized by Region 4 of the σ subunit of RNA polymerase (RNAP). We demonstrate that σ Region 4 is required for BvgA activation of PfhaB, a hallmark of Class II activation. In contrast, the promoter-proximal BvgA binding site at Pfim3 includes the − 35 region, which is composed of a tract of cytosines that lacks specific sequence information. We demonstrate that σ Region 4 is not required for BvgA activation at Pfim3. Nonetheless, Region 4 mutations that impair its typical interactions with core and with the − 35 DNA affect Pfim3 transcription. Hydroxyl radical cleavage using RNAP with σD581C–FeBABE positions Region 4 near the − 35 region of Pfim3; cleavage using RNAP with α276C–FeBABE or α302C–FeBABE also positions an α subunit C-terminal domain within the − 35 region, on a different helical face from the promoter-proximal BvgA~P dimer. Our results suggest that the − 35 region of Pfim3 accommodates a BvgA~P dimer, an α subunit C-terminal domain, and σ Region 4. Molecular modeling suggests how BvgA, σ Region 4, and α might coexist within this DNA in a conformation that suggests a novel mechanism of activation." @default.
• W1991159698 created "2016-06-24" @default.
• W1991159698 creator A5008258237 @default.
• W1991159698 creator A5025589456 @default.
• W1991159698 creator A5033112541 @default.
• W1991159698 creator A5066847489 @default.
• W1991159698 creator A5085753265 @default.
• W1991159698 creator A5091234712 @default.
• W1991159698 date "2011-06-01" @default.
• W1991159698 modified "2023-10-03" @default.
• W1991159698 title "Different Requirements for σ Region 4 in BvgA Activation of the Bordetella pertussis Promoters Pfim3 and PfhaB" @default.
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