FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991188051> ?p ?o ?g. }

• W1991188051 endingPage "S377" @default.
• W1991188051 startingPage "S376" @default.
• W1991188051 abstract "Radiation therapy is an established local therapy for muscle invasive transitional cell carcinoma of urinary bladder. Radiation has been shown to improve gene transduction and transgene integration for gene therapy. On the other hand, cancer cells expressing a cytotoxic gene may not repair radiation induced DNA damage. Decrease or loss of CAR (coxsackie-adenoviral receptor) expression is associated with higher grade and stage, metastatic potential and poorer prognosis in bladder cancer. The purpose of this study is to evaluate the efficacy of a novel CAR independent Ad (adenoviral) vector suicide gene therapy in combination with radiation for bladder cancer. Three human bladder cancer cell lines: 5637 (CAR-positive), T24 (CAR deficient) and TCCSUP (CAR deficient) were used. Increasing doses of radiation (2.5, 5, 7.5 and 10 Gy) were delivered to a monolayer of cancer cells. 2.5 Gy was selected for use in combination (radio-gene therapy) treatment. The therapeutic chimeric Ad vector (Ad5/F35) incorporates the Ad35 fiber gene into the original Ad5 backbone and expresses HSV-tk (herpes-simplex virus thymidine kinase gene) driven by the CMV (cytomegalovirus) promoter. Efficacy has been previously established in combination with ganciclovir (GCV) in CAR+ and CAR- bladder cancer cells. The efficacy of combination therapy was evaluated using increasing MOI’s (multiplicity of infections) of Ad5/F35HSV-tk followed by a single dose of radiation (2.5 Gy) that was delivered prior to initiation of GCV (ganciclovir) treatment. The MTT assay was used to assess cell viability. Induction of apoptosis was evaluated by annexin V-FITC. The effect on cell cycle and induction of apoptosis were analyzed using flow cytometry. In all three bladder cancer cell lines, anti-tumor effects were observed in a dose- dependent manner with the Ad5/F35HSV-tk vector. A 10-fold significant increase in anti-tumor effect was demonstrated in combination therapy (radio-gene therapy) when compared to gene therapy alone. Suicide gene therapy as monotherapy as well as in combination with radiation both caused a significant increase in G0/G1 arrest. However, radiation alone demonstrated G2/M arrest. Gene therapy alone demonstrated predominant induction of necrosis. Combination therapy showed higher early induction of apoptosis compared to gene therapy alone. The cell killing effect of this combination strategy, was similar in CAR-positive and -deficient bladder cancer cells. We have demonstrated that the combination radiation and suicide gene therapy elicits significantly greater anti-tumor effect compared to suicide gene therapy alone in both CAR-positive and -deficient bladder cancer cells. Combination radio-gene therapy leads to G0/G1 arrest in cell cycle and predominantly early induction of apoptosis. We are currently evaluating this novel treatment strategy in animal models in order to demonstrate a rationale for enhanced local-regional control in the bladder-sparing therapeutic approach for muscle invasive bladder cancer" @default.
• W1991188051 created "2016-06-24" @default.
• W1991188051 creator A5005242855 @default.
• W1991188051 creator A5017770836 @default.
• W1991188051 creator A5024670184 @default.
• W1991188051 creator A5037000397 @default.
• W1991188051 creator A5047315296 @default.
• W1991188051 creator A5048604566 @default.
• W1991188051 creator A5056602302 @default.
• W1991188051 creator A5065202811 @default.
• W1991188051 creator A5091208634 @default.
• W1991188051 date "2004-09-01" @default.
• W1991188051 modified "2023-09-26" @default.
• W1991188051 title "Novel therapeutic approach for bladder cancer: Synergistic effects with combined radiation and suicide gene therapy using a chimeric adenovirus vector" @default.
• W1991188051 doi "https://doi.org/10.1016/j.ijrobp.2004.07.232" @default.
• W1991188051 hasPublicationYear "2004" @default.
• W1991188051 type Work @default.
• W1991188051 sameAs 1991188051 @default.
• W1991188051 citedByCount "2" @default.
• W1991188051 countsByYear W19911880512023 @default.
• W1991188051 crossrefType "journal-article" @default.
• W1991188051 hasAuthorship W1991188051A5005242855 @default.
• W1991188051 hasAuthorship W1991188051A5017770836 @default.
• W1991188051 hasAuthorship W1991188051A5024670184 @default.
• W1991188051 hasAuthorship W1991188051A5037000397 @default.
• W1991188051 hasAuthorship W1991188051A5047315296 @default.
• W1991188051 hasAuthorship W1991188051A5048604566 @default.
• W1991188051 hasAuthorship W1991188051A5056602302 @default.
• W1991188051 hasAuthorship W1991188051A5065202811 @default.
• W1991188051 hasAuthorship W1991188051A5091208634 @default.
• W1991188051 hasConcept C104317684 @default.
• W1991188051 hasConcept C111599444 @default.
• W1991188051 hasConcept C121608353 @default.
• W1991188051 hasConcept C126322002 @default.
• W1991188051 hasConcept C203014093 @default.
• W1991188051 hasConcept C203233990 @default.
• W1991188051 hasConcept C2522874641 @default.
• W1991188051 hasConcept C2777965375 @default.
• W1991188051 hasConcept C2779820661 @default.
• W1991188051 hasConcept C2780352672 @default.
• W1991188051 hasConcept C2781196997 @default.
• W1991188051 hasConcept C32470452 @default.
• W1991188051 hasConcept C34981463 @default.
• W1991188051 hasConcept C40767141 @default.
• W1991188051 hasConcept C502942594 @default.
• W1991188051 hasConcept C509974204 @default.
• W1991188051 hasConcept C55493867 @default.
• W1991188051 hasConcept C71924100 @default.
• W1991188051 hasConcept C86803240 @default.
• W1991188051 hasConceptScore W1991188051C104317684 @default.
• W1991188051 hasConceptScore W1991188051C111599444 @default.
• W1991188051 hasConceptScore W1991188051C121608353 @default.
• W1991188051 hasConceptScore W1991188051C126322002 @default.
• W1991188051 hasConceptScore W1991188051C203014093 @default.
• W1991188051 hasConceptScore W1991188051C203233990 @default.
• W1991188051 hasConceptScore W1991188051C2522874641 @default.
• W1991188051 hasConceptScore W1991188051C2777965375 @default.
• W1991188051 hasConceptScore W1991188051C2779820661 @default.
• W1991188051 hasConceptScore W1991188051C2780352672 @default.
• W1991188051 hasConceptScore W1991188051C2781196997 @default.
• W1991188051 hasConceptScore W1991188051C32470452 @default.
• W1991188051 hasConceptScore W1991188051C34981463 @default.
• W1991188051 hasConceptScore W1991188051C40767141 @default.
• W1991188051 hasConceptScore W1991188051C502942594 @default.
• W1991188051 hasConceptScore W1991188051C509974204 @default.
• W1991188051 hasConceptScore W1991188051C55493867 @default.
• W1991188051 hasConceptScore W1991188051C71924100 @default.
• W1991188051 hasConceptScore W1991188051C86803240 @default.
• W1991188051 hasIssue "1" @default.
• W1991188051 hasLocation W19911880511 @default.
• W1991188051 hasOpenAccess W1991188051 @default.
• W1991188051 hasPrimaryLocation W19911880511 @default.
• W1991188051 hasRelatedWork W184467611 @default.
• W1991188051 hasRelatedWork W2009123070 @default.
• W1991188051 hasRelatedWork W2083919673 @default.
• W1991188051 hasRelatedWork W2088877272 @default.
• W1991188051 hasRelatedWork W2142305690 @default.
• W1991188051 hasRelatedWork W2319106492 @default.
• W1991188051 hasRelatedWork W2323062703 @default.
• W1991188051 hasRelatedWork W2323605700 @default.
• W1991188051 hasRelatedWork W2327229791 @default.
• W1991188051 hasRelatedWork W2907354303 @default.
• W1991188051 hasVolume "60" @default.
• W1991188051 isParatext "false" @default.
• W1991188051 isRetracted "false" @default.
• W1991188051 magId "1991188051" @default.
• W1991188051 workType "article" @default.