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• W1991195495 abstract "Although repair of double-strand breaks (DSBs) by gene conversion is the most accurate way to repair such lesions, in budding yeast there is a 1,000-fold increase in accompanying mutations, including interchromosomal template switches (ICTS) involving highly mismatched (homeologous) ectopic sequences. Although such events are rare and appear at a rate of 2 × 10(-7) when template jumps occur between 71% identical sequences, they are surprisingly frequent (0.3% of all repair events) when the second template is identical to the first, revealing the remarkable instability of repair DNA synthesis. With homeologous donors, ICTS uses microhomologies as small as 2 bp. Cells lacking mismatch repair proteins Msh6 and Mlh1 form chimeric recombinants with two distinct patches of microhomology, implying that these proteins are crucial for strand discrimination of heteroduplex DNA formed during ICTS. We identify the chromatin remodeler Rdh54 as the first protein required for template switching that does not affect simple gene conversion." @default.
• W1991195495 created "2016-06-24" @default.
• W1991195495 creator A5036925364 @default.
• W1991195495 creator A5048113506 @default.
• W1991195495 date "2014-08-01" @default.
• W1991195495 modified "2023-10-05" @default.
• W1991195495 title "Frequent Interchromosomal Template Switches during Gene Conversion in S. cerevisiae" @default.
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• W1991195495 doi "https://doi.org/10.1016/j.molcel.2014.06.025" @default.
• W1991195495 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4150392" @default.
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