Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991221600> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W1991221600 endingPage "373" @default.
- W1991221600 startingPage "369" @default.
- W1991221600 abstract "In vitro assays using isolated fish cells are increasingly used to measure the induction of cytochrome P4501A (CYP1A) and its associated enzyme activity, 7-ethoxyresorufin-O-deethylase (EROD). Compared to permanent cell lines, primary hepatocyte cultures represent an in vitro system that is closer to the in vivo situation, and, therefore, may be a particularly suitable model to study physiological and xenobiotic regulation of CYP1A expression. The application of conventional EROD assays to primary hepatocyte cultures, however, suffers from some disadvantages such as the labour-intensity and the need for high cell numbers per sample. In this report, we present an approach for assessment of EROD activity in teleost hepatocytes cultured in 48-well plates without the need for preparation of microsomes. The assay is based on the method of (Kennedy, S. W., Lorenzen, A., James, C. A. and Collins, B. T. (1993) Anal. Biochem. 211, 102–112.) but employs live instead of dead cells. For EROD detection, 7-ethoxyresorufin is added directly to the live cells and its metabolism to resorufin is observed by repeated measurements over 20 min in a fluorescence plate reader. The assay does not require the addition of exogenous NADPH. Resorufin utilization through cytoplasmic DT-diaphorase has to be inhibited by addition of dicoumarol. Disturbance by conjugate formation accounts to not more than 20% of total resorufin formed. After measurement, the cells can be washed and, after continued incubation, can be used for further assays." @default.
- W1991221600 created "2016-06-24" @default.
- W1991221600 creator A5030146621 @default.
- W1991221600 creator A5037610631 @default.
- W1991221600 creator A5037830581 @default.
- W1991221600 creator A5085551019 @default.
- W1991221600 date "1998-07-01" @default.
- W1991221600 modified "2023-10-17" @default.
- W1991221600 title "Microassay for rapid measurement of 7-ethoxyresorufin-O-deethylase activity in intact fish hepatocytes" @default.
- W1991221600 cites W1970118403 @default.
- W1991221600 cites W1995584082 @default.
- W1991221600 cites W2017729229 @default.
- W1991221600 cites W2020597160 @default.
- W1991221600 cites W2039259977 @default.
- W1991221600 cites W2047778256 @default.
- W1991221600 cites W2050719731 @default.
- W1991221600 cites W2062787844 @default.
- W1991221600 cites W2080569807 @default.
- W1991221600 cites W2152374623 @default.
- W1991221600 cites W2169744472 @default.
- W1991221600 doi "https://doi.org/10.1016/s0141-1136(97)00039-1" @default.
- W1991221600 hasPublicationYear "1998" @default.
- W1991221600 type Work @default.
- W1991221600 sameAs 1991221600 @default.
- W1991221600 citedByCount "48" @default.
- W1991221600 countsByYear W19912216002012 @default.
- W1991221600 countsByYear W19912216002014 @default.
- W1991221600 countsByYear W19912216002015 @default.
- W1991221600 countsByYear W19912216002016 @default.
- W1991221600 countsByYear W19912216002018 @default.
- W1991221600 countsByYear W19912216002019 @default.
- W1991221600 crossrefType "journal-article" @default.
- W1991221600 hasAuthorship W1991221600A5030146621 @default.
- W1991221600 hasAuthorship W1991221600A5037610631 @default.
- W1991221600 hasAuthorship W1991221600A5037830581 @default.
- W1991221600 hasAuthorship W1991221600A5085551019 @default.
- W1991221600 hasConcept C115448650 @default.
- W1991221600 hasConcept C150903083 @default.
- W1991221600 hasConcept C153911025 @default.
- W1991221600 hasConcept C181199279 @default.
- W1991221600 hasConcept C185592680 @default.
- W1991221600 hasConcept C190062978 @default.
- W1991221600 hasConcept C202751555 @default.
- W1991221600 hasConcept C207001950 @default.
- W1991221600 hasConcept C25642318 @default.
- W1991221600 hasConcept C2776200302 @default.
- W1991221600 hasConcept C2777990912 @default.
- W1991221600 hasConcept C2780768313 @default.
- W1991221600 hasConcept C526171541 @default.
- W1991221600 hasConcept C54355233 @default.
- W1991221600 hasConcept C55493867 @default.
- W1991221600 hasConcept C75520062 @default.
- W1991221600 hasConcept C81885089 @default.
- W1991221600 hasConcept C86803240 @default.
- W1991221600 hasConcept C87644729 @default.
- W1991221600 hasConceptScore W1991221600C115448650 @default.
- W1991221600 hasConceptScore W1991221600C150903083 @default.
- W1991221600 hasConceptScore W1991221600C153911025 @default.
- W1991221600 hasConceptScore W1991221600C181199279 @default.
- W1991221600 hasConceptScore W1991221600C185592680 @default.
- W1991221600 hasConceptScore W1991221600C190062978 @default.
- W1991221600 hasConceptScore W1991221600C202751555 @default.
- W1991221600 hasConceptScore W1991221600C207001950 @default.
- W1991221600 hasConceptScore W1991221600C25642318 @default.
- W1991221600 hasConceptScore W1991221600C2776200302 @default.
- W1991221600 hasConceptScore W1991221600C2777990912 @default.
- W1991221600 hasConceptScore W1991221600C2780768313 @default.
- W1991221600 hasConceptScore W1991221600C526171541 @default.
- W1991221600 hasConceptScore W1991221600C54355233 @default.
- W1991221600 hasConceptScore W1991221600C55493867 @default.
- W1991221600 hasConceptScore W1991221600C75520062 @default.
- W1991221600 hasConceptScore W1991221600C81885089 @default.
- W1991221600 hasConceptScore W1991221600C86803240 @default.
- W1991221600 hasConceptScore W1991221600C87644729 @default.
- W1991221600 hasIssue "1-5" @default.
- W1991221600 hasLocation W19912216001 @default.
- W1991221600 hasOpenAccess W1991221600 @default.
- W1991221600 hasPrimaryLocation W19912216001 @default.
- W1991221600 hasRelatedWork W1569106110 @default.
- W1991221600 hasRelatedWork W1978392142 @default.
- W1991221600 hasRelatedWork W1986974279 @default.
- W1991221600 hasRelatedWork W1991221600 @default.
- W1991221600 hasRelatedWork W2036206840 @default.
- W1991221600 hasRelatedWork W2413189091 @default.
- W1991221600 hasRelatedWork W2478188498 @default.
- W1991221600 hasRelatedWork W2588177540 @default.
- W1991221600 hasRelatedWork W3024317301 @default.
- W1991221600 hasRelatedWork W92954909 @default.
- W1991221600 hasVolume "46" @default.
- W1991221600 isParatext "false" @default.
- W1991221600 isRetracted "false" @default.
- W1991221600 magId "1991221600" @default.
- W1991221600 workType "article" @default.