FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991229285> ?p ?o ?g. }

• W1991229285 endingPage "694" @default.
• W1991229285 startingPage "686" @default.
• W1991229285 abstract "Efficient delivery of iron is critically dependent on the binding of diferric human serum transferrin (hTF) to its specific receptor (TFR) on the surface of actively dividing cells. Internalization of the complex into an endosome precedes iron removal. The return of hTF to the blood to continue the iron delivery cycle relies on the maintenance of the interaction between apohTF and the TFR after exposure to endosomal pH (≤6.0). Identification of the specific residues accounting for the pH-sensitive nanomolar affinity with which hTF binds to TFR throughout the cycle is important to fully understand the iron delivery process. Alanine substitution of 11 charged hTF residues identified by available structures and modeling studies allowed evaluation of the role of each in (1) binding of hTF to the TFR and (2) TFR-mediated iron release. Six hTF mutants (R50A, R352A, D356A, E357A, E367A, and K511A) competed poorly with biotinylated diferric hTF for binding to TFR. In particular, we show that Asp356 in the C-lobe of hTF is essential to the formation of a stable hTF–TFR complex: mutation of Asp356 in the monoferric C-lobe hTF background prevented the formation of the stoichiometric 2:2 (hTF:TFR monomer) complex. Moreover, mutation of three residues (Asp356, Glu367, and Lys511), whether in the diferric or monoferric C-lobe hTF, significantly affected iron release when in complex with the TFR. Thus, mutagenesis of charged hTF residues has allowed identification of a number of residues that are critical to formation of and release of iron from the hTF–TFR complex." @default.
• W1991229285 created "2016-06-24" @default.
• W1991229285 creator A5003824259 @default.
• W1991229285 creator A5013783603 @default.
• W1991229285 creator A5034086813 @default.
• W1991229285 creator A5038635870 @default.
• W1991229285 creator A5056671277 @default.
• W1991229285 creator A5069612170 @default.
• W1991229285 creator A5071151203 @default.
• W1991229285 creator A5075970098 @default.
• W1991229285 date "2012-01-06" @default.
• W1991229285 modified "2023-10-18" @default.
• W1991229285 title "Ionic Residues of Human Serum Transferrin Affect Binding to the Transferrin Receptor and Iron Release" @default.
• W1991229285 cites W114062130 @default.
• W1991229285 cites W146253052 @default.
• W1991229285 cites W1967684793 @default.
• W1991229285 cites W1971724949 @default.
• W1991229285 cites W1977457791 @default.
• W1991229285 cites W1986296275 @default.
• W1991229285 cites W2011357252 @default.
• W1991229285 cites W2016915374 @default.
• W1991229285 cites W2027978484 @default.
• W1991229285 cites W2031856351 @default.
• W1991229285 cites W2046818843 @default.
• W1991229285 cites W2048578960 @default.
• W1991229285 cites W2059519530 @default.
• W1991229285 cites W2090296940 @default.
• W1991229285 cites W2113952988 @default.
• W1991229285 cites W2162927747 @default.
• W1991229285 cites W2167563458 @default.
• W1991229285 doi "https://doi.org/10.1021/bi201661g" @default.
• W1991229285 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3267578" @default.
• W1991229285 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22191507" @default.
• W1991229285 hasPublicationYear "2012" @default.
• W1991229285 type Work @default.
• W1991229285 sameAs 1991229285 @default.
• W1991229285 citedByCount "18" @default.
• W1991229285 countsByYear W19912292852012 @default.
• W1991229285 countsByYear W19912292852013 @default.
• W1991229285 countsByYear W19912292852014 @default.
• W1991229285 countsByYear W19912292852015 @default.
• W1991229285 countsByYear W19912292852016 @default.
• W1991229285 countsByYear W19912292852018 @default.
• W1991229285 countsByYear W19912292852019 @default.
• W1991229285 countsByYear W19912292852022 @default.
• W1991229285 crossrefType "journal-article" @default.
• W1991229285 hasAuthorship W1991229285A5003824259 @default.
• W1991229285 hasAuthorship W1991229285A5013783603 @default.
• W1991229285 hasAuthorship W1991229285A5034086813 @default.
• W1991229285 hasAuthorship W1991229285A5038635870 @default.
• W1991229285 hasAuthorship W1991229285A5056671277 @default.
• W1991229285 hasAuthorship W1991229285A5069612170 @default.
• W1991229285 hasAuthorship W1991229285A5071151203 @default.
• W1991229285 hasAuthorship W1991229285A5075970098 @default.
• W1991229285 hasBestOaLocation W19912292852 @default.
• W1991229285 hasConcept C102747710 @default.
• W1991229285 hasConcept C104317684 @default.
• W1991229285 hasConcept C12554922 @default.
• W1991229285 hasConcept C139770010 @default.
• W1991229285 hasConcept C143065580 @default.
• W1991229285 hasConcept C170493617 @default.
• W1991229285 hasConcept C185592680 @default.
• W1991229285 hasConcept C22814914 @default.
• W1991229285 hasConcept C32264632 @default.
• W1991229285 hasConcept C55493867 @default.
• W1991229285 hasConcept C86803240 @default.
• W1991229285 hasConceptScore W1991229285C102747710 @default.
• W1991229285 hasConceptScore W1991229285C104317684 @default.
• W1991229285 hasConceptScore W1991229285C12554922 @default.
• W1991229285 hasConceptScore W1991229285C139770010 @default.
• W1991229285 hasConceptScore W1991229285C143065580 @default.
• W1991229285 hasConceptScore W1991229285C170493617 @default.
• W1991229285 hasConceptScore W1991229285C185592680 @default.
• W1991229285 hasConceptScore W1991229285C22814914 @default.
• W1991229285 hasConceptScore W1991229285C32264632 @default.
• W1991229285 hasConceptScore W1991229285C55493867 @default.
• W1991229285 hasConceptScore W1991229285C86803240 @default.
• W1991229285 hasIssue "2" @default.
• W1991229285 hasLocation W19912292851 @default.
• W1991229285 hasLocation W19912292852 @default.
• W1991229285 hasLocation W19912292853 @default.
• W1991229285 hasLocation W19912292854 @default.
• W1991229285 hasOpenAccess W1991229285 @default.
• W1991229285 hasPrimaryLocation W19912292851 @default.
• W1991229285 hasRelatedWork W138218014 @default.
• W1991229285 hasRelatedWork W1754259525 @default.
• W1991229285 hasRelatedWork W1985279299 @default.
• W1991229285 hasRelatedWork W1991250386 @default.
• W1991229285 hasRelatedWork W2001564713 @default.
• W1991229285 hasRelatedWork W2025935935 @default.
• W1991229285 hasRelatedWork W2033602362 @default.
• W1991229285 hasRelatedWork W2066822786 @default.
• W1991229285 hasRelatedWork W2090492705 @default.
• W1991229285 hasRelatedWork W2358166573 @default.
• W1991229285 hasVolume "51" @default.
• W1991229285 isParatext "false" @default.
• W1991229285 isRetracted "false" @default.
• W1991229285 magId "1991229285" @default.

• next