FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991232824> ?p ?o ?g. }

• W1991232824 endingPage "261" @default.
• W1991232824 startingPage "255" @default.
• W1991232824 abstract "Cytochrome P450 (P450) 2D6 is a polymorphic human enzyme involved in the oxidation of >50 drugs, most of which contain a basic nitrogen. In confirmation of previous work by others, substitutions at Asp301 decreased rates of substrate oxidation by P450 2D6. An anionic residue (Asp, Glu) at this position was found to be important in proper protein folding and heme incorporation, and positively charged residues were particularly disruptive in bacterial and also in baculovirus expression systems. Truncation of 20 N-terminal amino acids had no significant effect on catalytic activity except to attenuate P450 2D6 interaction with membranes and NADPH-P450 reductase. The truncation of the N-terminus increased the level of bacterial expression of wild-type P450 2D6 (Asp301) but markedly reduced expression of all codon 301 mutants, including Glu301. Reduction of ferric P450 2D6 by NADPH-P450 reductase was enhanced in the presence of the prototypic substrate bufuralol. Bacterial flavodoxin, an NADPH-P450 reductase homolog, binds tightly to P450 2D6 but is inefficient in electron transfer to the heme. These results collectively indicate that the acidic residue at position 301 in P450 2D6 has a structural role in addition to any in substrate binding and that the N-terminus of P450 2D6 is relatively unimportant to catalytic activity beyond a role in facilitating binding to NADPH-P450 reductase." @default.
• W1991232824 created "2016-06-24" @default.
• W1991232824 creator A5015815568 @default.
• W1991232824 creator A5025977173 @default.
• W1991232824 creator A5032350690 @default.
• W1991232824 date "2001-09-01" @default.
• W1991232824 modified "2023-09-27" @default.
• W1991232824 title "Heterologous Expression of Cytochrome P450 2D6 Mutants, Electron Transfer, and Catalysis of Bufuralol Hydroxylation: The Role of Aspartate 301 in Structural Integrity" @default.
• W1991232824 cites W1509690851 @default.
• W1991232824 cites W1522760620 @default.
• W1991232824 cites W1524323459 @default.
• W1991232824 cites W1579297624 @default.
• W1991232824 cites W1588794217 @default.
• W1991232824 cites W1650427240 @default.
• W1991232824 cites W1966533731 @default.
• W1991232824 cites W1969215912 @default.
• W1991232824 cites W1971796402 @default.
• W1991232824 cites W1973407453 @default.
• W1991232824 cites W1980165418 @default.
• W1991232824 cites W1984700929 @default.
• W1991232824 cites W1985014958 @default.
• W1991232824 cites W1991058493 @default.
• W1991232824 cites W1992822028 @default.
• W1991232824 cites W1998858855 @default.
• W1991232824 cites W2003916390 @default.
• W1991232824 cites W2008984280 @default.
• W1991232824 cites W2009712270 @default.
• W1991232824 cites W2010445932 @default.
• W1991232824 cites W2011048896 @default.
• W1991232824 cites W2014362986 @default.
• W1991232824 cites W2021842042 @default.
• W1991232824 cites W2029053882 @default.
• W1991232824 cites W2029810822 @default.
• W1991232824 cites W2034551014 @default.
• W1991232824 cites W2035581695 @default.
• W1991232824 cites W2039249350 @default.
• W1991232824 cites W2045705381 @default.
• W1991232824 cites W2047362094 @default.
• W1991232824 cites W2048808087 @default.
• W1991232824 cites W2061129458 @default.
• W1991232824 cites W2061786447 @default.
• W1991232824 cites W2064638034 @default.
• W1991232824 cites W2064926901 @default.
• W1991232824 cites W2072170860 @default.
• W1991232824 cites W2074402745 @default.
• W1991232824 cites W2076943035 @default.
• W1991232824 cites W2084686194 @default.
• W1991232824 cites W2153834789 @default.
• W1991232824 cites W2162546799 @default.
• W1991232824 cites W2163891284 @default.
• W1991232824 cites W2228413566 @default.
• W1991232824 cites W2329354175 @default.
• W1991232824 doi "https://doi.org/10.1006/abbi.2001.2510" @default.
• W1991232824 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11556812" @default.
• W1991232824 hasPublicationYear "2001" @default.
• W1991232824 type Work @default.
• W1991232824 sameAs 1991232824 @default.
• W1991232824 citedByCount "48" @default.
• W1991232824 countsByYear W19912328242012 @default.
• W1991232824 countsByYear W19912328242013 @default.
• W1991232824 countsByYear W19912328242015 @default.
• W1991232824 countsByYear W19912328242017 @default.
• W1991232824 countsByYear W19912328242018 @default.
• W1991232824 countsByYear W19912328242019 @default.
• W1991232824 countsByYear W19912328242020 @default.
• W1991232824 countsByYear W19912328242021 @default.
• W1991232824 crossrefType "journal-article" @default.
• W1991232824 hasAuthorship W1991232824A5015815568 @default.
• W1991232824 hasAuthorship W1991232824A5025977173 @default.
• W1991232824 hasAuthorship W1991232824A5032350690 @default.
• W1991232824 hasConcept C104317684 @default.
• W1991232824 hasConcept C108305142 @default.
• W1991232824 hasConcept C134651460 @default.
• W1991232824 hasConcept C143065580 @default.
• W1991232824 hasConcept C148292235 @default.
• W1991232824 hasConcept C171305022 @default.
• W1991232824 hasConcept C181199279 @default.
• W1991232824 hasConcept C185592680 @default.
• W1991232824 hasConcept C24586158 @default.
• W1991232824 hasConcept C2776217839 @default.
• W1991232824 hasConcept C2781109383 @default.
• W1991232824 hasConcept C40767141 @default.
• W1991232824 hasConcept C526171541 @default.
• W1991232824 hasConcept C55493867 @default.
• W1991232824 hasConcept C71240020 @default.
• W1991232824 hasConceptScore W1991232824C104317684 @default.
• W1991232824 hasConceptScore W1991232824C108305142 @default.
• W1991232824 hasConceptScore W1991232824C134651460 @default.
• W1991232824 hasConceptScore W1991232824C143065580 @default.
• W1991232824 hasConceptScore W1991232824C148292235 @default.
• W1991232824 hasConceptScore W1991232824C171305022 @default.
• W1991232824 hasConceptScore W1991232824C181199279 @default.
• W1991232824 hasConceptScore W1991232824C185592680 @default.
• W1991232824 hasConceptScore W1991232824C24586158 @default.
• W1991232824 hasConceptScore W1991232824C2776217839 @default.
• W1991232824 hasConceptScore W1991232824C2781109383 @default.
• W1991232824 hasConceptScore W1991232824C40767141 @default.
• W1991232824 hasConceptScore W1991232824C526171541 @default.

• next