FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991273887> ?p ?o ?g. }

• W1991273887 endingPage "2181" @default.
• W1991273887 startingPage "2181" @default.
• W1991273887 abstract "In a mouse model of oxygen-induced retinopathy (OIR), a well-established model of retinopathy of prematurity (ROP), blocking beta-adrenoreceptors (β-ARs), and, in particular, β2-ARs, counteracts retinal responses to hypoxia. In the present work, we determined the effects of the β-AR agonist isoproterenol on retinal angiogenesis and β-AR signaling to better clarify the role of sympathetic transmission in ROP.Isoproterenol was administered subcutaneously. Protein kinase A activity was determined by a colorimetric assay to assess drug effectiveness. Blood pressure and heart-to-body weight ratio were measured. Vascular endothelial growth factor (VEGF) and norepinephrine were measured with ELISA. Retinal neovascularization was assessed by CD31 immunohistochemistry. β-AR-coupled adenylyl cyclase (AC) activity was measured with a competition assay. β-ARs, G-protein-coupled receptor kinase (GRK)2, and β-arrestins were determined by Western blot. Association of β-arrestins with β2-ARs was assessed by immunoprecipitation.Isoproterenol-induced modulation of protein kinase A activity suggests that the drug was effective at the receptor level. Isoproterenol did not affect cardiovascular parameters, but decreased retinal levels of VEGF and reduced pathogenic neovascularization, likely through an influence on sympathetic transmission. In fact, isoproterenol downregulated β2-AR expression, recovered the hypoxia-induced increase in β-AR-coupled AC activity, and increased GRK2 and β-arrestins, which promote β-AR desensitization through the uncoupling of G-protein-coupled receptors from G proteins. Immunoprecipitation studies demonstrated that β-AR desensitization involved β2-ARs.Our findings suggest that hypoxia-induced retinal neovascularization depends at least in part on increased sympathetic transmission, as reduction of sympathetic drive by agonist-induced β2-AR desensitization inhibits some of the hallmarks of OIR." @default.
• W1991273887 created "2016-06-24" @default.
• W1991273887 creator A5002007850 @default.
• W1991273887 creator A5056180095 @default.
• W1991273887 creator A5057831734 @default.
• W1991273887 creator A5058669578 @default.
• W1991273887 creator A5061332336 @default.
• W1991273887 creator A5066998394 @default.
• W1991273887 date "2012-04-24" @default.
• W1991273887 modified "2023-10-18" @default.
• W1991273887 title "Beta-Adrenoreceptor Agonism Influences Retinal Responses to Hypoxia in a Model of Retinopathy of Prematurity" @default.
• W1991273887 cites W1518582333 @default.
• W1991273887 cites W1523082029 @default.
• W1991273887 cites W1538850399 @default.
• W1991273887 cites W1554753999 @default.
• W1991273887 cites W1645271935 @default.
• W1991273887 cites W1863660946 @default.
• W1991273887 cites W1941976899 @default.
• W1991273887 cites W1966126044 @default.
• W1991273887 cites W1966525626 @default.
• W1991273887 cites W1967412797 @default.
• W1991273887 cites W1968553219 @default.
• W1991273887 cites W1970046253 @default.
• W1991273887 cites W1970607218 @default.
• W1991273887 cites W1977401065 @default.
• W1991273887 cites W1977810373 @default.
• W1991273887 cites W1983951051 @default.
• W1991273887 cites W1985043568 @default.
• W1991273887 cites W1988043055 @default.
• W1991273887 cites W1988721089 @default.
• W1991273887 cites W199005441 @default.
• W1991273887 cites W1994438268 @default.
• W1991273887 cites W1995904975 @default.
• W1991273887 cites W1998558235 @default.
• W1991273887 cites W1998672817 @default.
• W1991273887 cites W2004845281 @default.
• W1991273887 cites W2008265510 @default.
• W1991273887 cites W2015824448 @default.
• W1991273887 cites W2016803030 @default.
• W1991273887 cites W2017883181 @default.
• W1991273887 cites W2019575834 @default.
• W1991273887 cites W2020083247 @default.
• W1991273887 cites W2020818891 @default.
• W1991273887 cites W202196835 @default.
• W1991273887 cites W2026134327 @default.
• W1991273887 cites W2026808548 @default.
• W1991273887 cites W2030106461 @default.
• W1991273887 cites W2030706201 @default.
• W1991273887 cites W2031099464 @default.
• W1991273887 cites W2031410052 @default.
• W1991273887 cites W2031583328 @default.
• W1991273887 cites W2032913982 @default.
• W1991273887 cites W2035801517 @default.
• W1991273887 cites W2050582843 @default.
• W1991273887 cites W2050846016 @default.
• W1991273887 cites W2051060614 @default.
• W1991273887 cites W2058585626 @default.
• W1991273887 cites W2062282128 @default.
• W1991273887 cites W2062932151 @default.
• W1991273887 cites W2064306453 @default.
• W1991273887 cites W2068777029 @default.
• W1991273887 cites W2068951249 @default.
• W1991273887 cites W2069098382 @default.
• W1991273887 cites W2071407489 @default.
• W1991273887 cites W2072322601 @default.
• W1991273887 cites W2073251204 @default.
• W1991273887 cites W2075563480 @default.
• W1991273887 cites W2076438136 @default.
• W1991273887 cites W2077783785 @default.
• W1991273887 cites W2086459628 @default.
• W1991273887 cites W2090144953 @default.
• W1991273887 cites W2091646436 @default.
• W1991273887 cites W2092001446 @default.
• W1991273887 cites W2095102966 @default.
• W1991273887 cites W2117539627 @default.
• W1991273887 cites W2135893198 @default.
• W1991273887 cites W2137374083 @default.
• W1991273887 cites W2143944465 @default.
• W1991273887 cites W2145951735 @default.
• W1991273887 cites W2158187739 @default.
• W1991273887 cites W2159009152 @default.
• W1991273887 cites W2160766402 @default.
• W1991273887 cites W2166957348 @default.
• W1991273887 cites W2169675069 @default.
• W1991273887 cites W2169811749 @default.
• W1991273887 cites W2415355820 @default.
• W1991273887 doi "https://doi.org/10.1167/iovs.11-9408" @default.
• W1991273887 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22410551" @default.
• W1991273887 hasPublicationYear "2012" @default.
• W1991273887 type Work @default.
• W1991273887 sameAs 1991273887 @default.
• W1991273887 citedByCount "41" @default.
• W1991273887 countsByYear W19912738872012 @default.
• W1991273887 countsByYear W19912738872013 @default.
• W1991273887 countsByYear W19912738872014 @default.
• W1991273887 countsByYear W19912738872015 @default.
• W1991273887 countsByYear W19912738872016 @default.
• W1991273887 countsByYear W19912738872017 @default.

• next