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• W1991283534 abstract "Abstract Carbon stable isotope ratios for six serum fatty acids (FA) are reported for human subjects on controlled fat diets to determine the range of natural isotope abundance and to demonstrate the leveling effect of a well‐controlled diet. Twenty‐nine subjects were randomly assigned to one of three controlled diets containing high, medium, or low fat. Diets were consumed for 8 wk. Serum samples were collected at baseline (0), 5, 6, 7, and 8 wk. FA were extracted and methylated. Isotope ratios were analyzed by high‐precision gas chromatography combustion‐isotope ratio mass spectrometry. At baseline, mean δ 13 C for 16:0 b , 16:1 a , 18:0 a , 18:1 c , 18:2n−6 d , and 20:4n−6 bc were −24.1, −21.7, −21.6, −25.6, −29.6, and −25.0%., respectively, with an average standard deviation of 1.9%.. Most δ 13 C decreased during the diet period and appeared to have stabilized by week 5 at −25.3, −21.9, −22.3, −26.5, −30.1, and −24.5%., respectively. Between‐subject variability decreased from 1.74 to 1.20%. on the controlled diets. Measurement variability was 0.53%.. The within‐subject variability during weeks 5–8 was 0.57%. (range of 0.32–0.84%.), showing a minimum biological fluctuation on controlled diets. There was no diet group effect on δ 13 C of serum FA. Except for 18:2, the δ 13 C of experimental diets was lower than that of serum FA, consistent with observations in animals. These data show that carbon isotope ratios stabilize in response to controlled diets within 5 wk, reflecting the isotope ratio of their dietary source, and establish isotope ratio fluctuations for endogenous compounds for future studies." @default.
• W1991283534 created "2016-06-24" @default.
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• W1991283534 date "1997-12-01" @default.
• W1991283534 modified "2023-09-30" @default.
• W1991283534 title "Fatty acid carbon isotope ratios in humans on controlled diets" @default.
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• W1991283534 doi "https://doi.org/10.1007/s11745-006-0161-6" @default.
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