FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991373985> ?p ?o ?g. }

• W1991373985 endingPage "729" @default.
• W1991373985 startingPage "720" @default.
• W1991373985 abstract "Bone tissue engineering (TE) aims to develop reproducible and predictive three-dimensional (3D) TE constructs, defined as cell-seeded scaffolds produced by a controlled in vitro process, to heal or replace damaged and nonfunctional bone. To control and assure the quality of the bone TE constructs, a prerequisite for regulatory authorization, there is a need to develop noninvasive analysis techniques to evaluate TE constructs and to monitor their behavior in real time during in vitro culturing. Most analysis techniques, however, are limited to destructive end-point analyses. This study investigates the use of the nontoxic alamarBlue® (AB) reagent, which is an indicator for metabolic cell activity, for monitoring the cellularity of 3D TE constructs in vitro as part of a bioreactor culturing processes. Within the field of TE, bioreactors have a huge potential in the translation of TE concepts to the clinic. Hence, the use of the AB reagent was evaluated not only in static cultures, but also in dynamic cultures in a perfusion bioreactor setup. Hereto, the AB assay was successfully integrated in the bioreactor-driven TE construct culture process in a noninvasive way. The obtained results indicate a linear correlation between the overall metabolic activity and the total DNA content of a scaffold upon seeding as well as during the initial stages of cell proliferation. This makes the AB reagent a powerful tool to follow-up bone TE constructs in real-time during static as well as dynamic 3D cultures. Hence, the AB reagent can be successfully used to monitor and predict cell confluence in a growing 3D TE construct." @default.
• W1991373985 created "2016-06-24" @default.
• W1991373985 creator A5000809601 @default.
• W1991373985 creator A5028343381 @default.
• W1991373985 creator A5035685689 @default.
• W1991373985 creator A5050555039 @default.
• W1991373985 creator A5059823367 @default.
• W1991373985 creator A5068263475 @default.
• W1991373985 creator A5087592855 @default.
• W1991373985 date "2013-09-01" @default.
• W1991373985 modified "2023-10-06" @default.
• W1991373985 title "Noninvasive Real-Time Monitoring by AlamarBlue^®During<i>In Vitro</i>Culture of Three-Dimensional Tissue-Engineered Bone Constructs" @default.
• W1991373985 cites W1480764297 @default.
• W1991373985 cites W1974083224 @default.
• W1991373985 cites W1974394551 @default.
• W1991373985 cites W1974610140 @default.
• W1991373985 cites W1975371675 @default.
• W1991373985 cites W1980116220 @default.
• W1991373985 cites W1986170848 @default.
• W1991373985 cites W1987893797 @default.
• W1991373985 cites W1990338759 @default.
• W1991373985 cites W1991221892 @default.
• W1991373985 cites W1991907360 @default.
• W1991373985 cites W1992948142 @default.
• W1991373985 cites W1996717996 @default.
• W1991373985 cites W2009915078 @default.
• W1991373985 cites W2015731765 @default.
• W1991373985 cites W2017394391 @default.
• W1991373985 cites W2021084410 @default.
• W1991373985 cites W2025360076 @default.
• W1991373985 cites W2026867268 @default.
• W1991373985 cites W2031091288 @default.
• W1991373985 cites W2037690890 @default.
• W1991373985 cites W2040914101 @default.
• W1991373985 cites W2043815471 @default.
• W1991373985 cites W2045172046 @default.
• W1991373985 cites W2046527972 @default.
• W1991373985 cites W2049165452 @default.
• W1991373985 cites W2049186245 @default.
• W1991373985 cites W2051484088 @default.
• W1991373985 cites W2052115118 @default.
• W1991373985 cites W2059962989 @default.
• W1991373985 cites W2067971473 @default.
• W1991373985 cites W2070773381 @default.
• W1991373985 cites W2071952696 @default.
• W1991373985 cites W2081639853 @default.
• W1991373985 cites W2088733234 @default.
• W1991373985 cites W2091278250 @default.
• W1991373985 cites W2092662596 @default.
• W1991373985 cites W2111903919 @default.
• W1991373985 cites W2114973216 @default.
• W1991373985 cites W2125889959 @default.
• W1991373985 cites W2130023194 @default.
• W1991373985 cites W2131942706 @default.
• W1991373985 cites W2154385453 @default.
• W1991373985 cites W2176300883 @default.
• W1991373985 cites W278789638 @default.
• W1991373985 cites W4252810190 @default.
• W1991373985 cites W80868934 @default.
• W1991373985 doi "https://doi.org/10.1089/ten.tec.2012.0601" @default.
• W1991373985 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3719443" @default.
• W1991373985 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23327780" @default.
• W1991373985 hasPublicationYear "2013" @default.
• W1991373985 type Work @default.
• W1991373985 sameAs 1991373985 @default.
• W1991373985 citedByCount "33" @default.
• W1991373985 countsByYear W19913739852013 @default.
• W1991373985 countsByYear W19913739852014 @default.
• W1991373985 countsByYear W19913739852015 @default.
• W1991373985 countsByYear W19913739852016 @default.
• W1991373985 countsByYear W19913739852017 @default.
• W1991373985 countsByYear W19913739852018 @default.
• W1991373985 countsByYear W19913739852019 @default.
• W1991373985 countsByYear W19913739852020 @default.
• W1991373985 countsByYear W19913739852021 @default.
• W1991373985 countsByYear W19913739852022 @default.
• W1991373985 countsByYear W19913739852023 @default.
• W1991373985 crossrefType "journal-article" @default.
• W1991373985 hasAuthorship W1991373985A5000809601 @default.
• W1991373985 hasAuthorship W1991373985A5028343381 @default.
• W1991373985 hasAuthorship W1991373985A5035685689 @default.
• W1991373985 hasAuthorship W1991373985A5050555039 @default.
• W1991373985 hasAuthorship W1991373985A5059823367 @default.
• W1991373985 hasAuthorship W1991373985A5068263475 @default.
• W1991373985 hasAuthorship W1991373985A5087592855 @default.
• W1991373985 hasBestOaLocation W19913739852 @default.
• W1991373985 hasConcept C111919701 @default.
• W1991373985 hasConcept C136229726 @default.
• W1991373985 hasConcept C147789679 @default.
• W1991373985 hasConcept C168170006 @default.
• W1991373985 hasConcept C178790620 @default.
• W1991373985 hasConcept C185592680 @default.
• W1991373985 hasConcept C192562407 @default.
• W1991373985 hasConcept C202751555 @default.
• W1991373985 hasConcept C40875361 @default.
• W1991373985 hasConcept C41008148 @default.
• W1991373985 hasConcept C49892992 @default.
• W1991373985 hasConcept C54355233 @default.

• next