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- W1991404656 abstract "The hydrolysis of adenylyl(3′→5′)adenosine (ApA) and guanylyl(3′→5′)adenosine (GpA) dinucleotides by the cytotoxic protein α‐sarcin has been studied. Quantitative analysis of the reaction has been performed through reverse‐phase chromatographic (HPLC) separation of the resulting products. The hydrolysis of the 3′‐5′ phosphodiester bond of these substrates yields the 2′‐3′ cyclic mononucleotide; this intermediate is converted into the corresponding 3′‐monophosphate derivative as the final product of the reaction. The values of the apparent Michaelis constant ( K M ), k cat and k cat / K M have also been calculated. The obtained results fit into a two‐step mechanism for the enzymatic activity of α‐sarcin and allow to consider this protein as a cyclizing RNase." @default.
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- W1991404656 date "1998-03-06" @default.
- W1991404656 modified "2023-10-18" @default.
- W1991404656 title "The cytotoxin α-sarcin behaves as a cyclizing ribonuclease" @default.
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- W1991404656 doi "https://doi.org/10.1016/s0014-5793(98)00137-9" @default.
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