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- W1991431036 abstract "Four γ-polyglutamate (γ-PGA)-producing Bacillus strains were isolated from a dan-douchi in China, all of which indicated biotin requirement for growth and a considerably high γ-glutamyltranspeptidase (γ-GTP) productivity. These strains carried a single plasmid species, and their molecular sizes and restriction patterns differed completely. Some of HindIII DNA fragments of dan-douchi plasmids strongly hybridized with a 1.7-kb TaqI DNA fragment of natto plasmid, pUH1, which encodes γ-GTP gene responsible for γ-PGA production, and their molecular sizes of homologous fragments were found to be similar. The hybridization analysis revealed that the structure gene of γ-GTP encoded on dan-douchi plasmid was found to greatly resemble that on natto plasmid. Furthermore, dan-douchi plasmid had a high degree of homology with DNA segment encoding the replication protein of pUH1. These results, therefore, strongly suggest that dan-douchi plasmid might be a functional plasmid as well as natto plasmid, and it should be considered that both natto and dan-douchi plasmids might develop from a common ancestral molecule." @default.
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- W1991431036 date "1993-01-01" @default.
- W1991431036 modified "2023-10-02" @default.
- W1991431036 title "Plasmid distribution in .GAMMA.-polyglutamate-producing Bacillus strains isolated from dan-douchi, a natto-like non-salty fermented soybean food in China." @default.
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- W1991431036 doi "https://doi.org/10.2323/jgam.39.75" @default.
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