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• W1991431804 abstract "DNA from 124 strains of Heterobasidion annosum was amplified using the core sequence of the M13 minisatellite region as primer. The strains were derived from 11 S-, 8 P- and 1 F-intersterility group populations originating in Scandinavia, Germany and Italy. Following electrophoresis the banding patterns of all isolates were compared and 23 fragments were scored. Average band-sharing indices (ABSI) were used to compare genetic diversity within and between populations. Genetic similarity of populations decreased with increasing geographical distance in the S group. In both the S and P intersterility groups, ABSI values were higher within than among populations (70·3 ± 2·3 s.d. and 66·4 ± 2·2, respectively in the S group and 77·0 ± 2·3 and 75·0 ± 2·4, respectively in the P group) indicating local differentiation. The higher values in the P than in the S group indicate that the P group is less variable. ABSI was much lower between S and P group populations (49·2 ± 2·5) than within either group. This result demonstrates the large divergence between these two intersterility groups. In the F population ABSI was higher (68 ± 3·1) than for the comparisons with the S- (50·1 ± 1·6) and P-groups (44·0 ± 2·4). By using discriminant analysis, the agreement between geographical population affiliation and that inferred from banding patterns of amplified DNA was 20·8% within the S group, and 14·9% within the P group. Chi-square test of the discriminant analysis indicated regional differentiation in the S but not in the P intersterility group. The agreement between methods was 98·4% when strains were classified to intersterility group by pairing tests or amplified DNA profiles. Results are consistent with a high degree of exchange between populations of the same intersterility group but low exchange between intersterility groups." @default.
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• W1991431804 date "1994-01-01" @default.
• W1991431804 modified "2023-10-17" @default.
• W1991431804 title "Intraspecific genetic variation in Heterobasidion annosum revealed by amplification of minisatellite DNA" @default.
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• W1991431804 doi "https://doi.org/10.1016/s0953-7562(09)80337-7" @default.
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