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• W1991437122 abstract "Steroid spin labels have been found to undergo rapid and sometimes anisotropic rotational motion in membranes and phospholipid vesicle preparations. N-oxyl-4′,4′-dimethyloxazolidine derivatives of 5α-androstan-3-one-17β-ol, 5β-androstan-3-one-17β-ol, and 5α-androstan-3-one were prepared according to the procedure described by Keana. The paramagnetic five-membered ring is rigidly attached to the steroid nucleus at the 3-position, and thus the resonance spectrum of the nitroxide group reflects the motion of the entire steroid nucleus. This rapid motion, with rotational diffusion frequencies of the order of 10 7 to 10 8 second -1 is considered to lend plausibility to models of membrane transport involving rotations and/or translations of carriers within the hydrophobic region of the membrane. The steroid spin labels also form a convenient class of probes for studying conformational changes in membranes, since ( a ) their resonance spectra often fall in the category of “intermediate immobilization,” which is most sensitive to small changes, and ( b ) these labels are quite soluble in a wide variety of membranes." @default.
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• W1991437122 date "1969-05-01" @default.
• W1991437122 modified "2023-09-26" @default.
• W1991437122 title "MOTION OF STEROID SPIN LABELS IN MEMBRANES" @default.
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• W1991437122 doi "https://doi.org/10.1073/pnas.63.1.16" @default.
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