FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991446568> ?p ?o ?g. }

• W1991446568 endingPage "80" @default.
• W1991446568 startingPage "70" @default.
• W1991446568 abstract "Aim It was the aim of this study to prepare purified DNA/PEI polyplexes, which are coated with hyaluronan to facilitate CD44 receptor mediated uptake of the DNA/PEI polyplex and to reduce unspecific interactions of the complex with negatively charged extracellular matrix components on the ocular surface. Methods Hyaluronans of different molecular weights (<10 kDa, 10–30 kDa and 30–50 kDa) were isolated after enzymatic degradation of high molecular weight hyaluronan via ultrafiltration by centrifugation. The influence of the different hyaluronans used for coating on the stability and transfection efficiency of the complexes was evaluated in vitro. Transfection and uptake studies were performed in human corneal epithelial (HCE) cells. CD44 receptor expression of this cell model was evaluated by immunohistochemistry. Results Coating of purified DNA/PEI polyplexes with low molecular weight hyaluronan (<10 kDa) facilitated receptor-mediated uptake via the CD44 receptor in HCE cells, increased complex stability in vitro, and effectively shielded the positive surface charges of the polyplex without decreasing its transfection efficiency. Higher molecular weights and larger amounts of hyaluronan in the complexes resulted in lesser improvements in the stability and transfection efficacy of the complexes. Conclusions Coating of polyplexes with low molecular weight hyaluronan is a promising strategy for gene delivery to the ocular surface, where CD44 receptor mediated uptake decreased cytotoxicity and reduced non-specific interactions with the negatively charged extracellular matrix components are considered beneficial for increased transfection efficiency of non-viral vectors. Copyright © 2007 John Wiley & Sons, Ltd." @default.
• W1991446568 created "2016-06-24" @default.
• W1991446568 creator A5024772304 @default.
• W1991446568 creator A5030998615 @default.
• W1991446568 creator A5061234170 @default.
• W1991446568 creator A5062744013 @default.
• W1991446568 creator A5065810893 @default.
• W1991446568 date "2007-01-01" @default.
• W1991446568 modified "2023-09-25" @default.
• W1991446568 title "Low molecular weight hyaluronan shielding of DNA/PEI polyplexes facilitates CD44 receptor mediated uptake in human corneal epithelial cells" @default.
• W1991446568 cites W1968113316 @default.
• W1991446568 cites W1976811878 @default.
• W1991446568 cites W1977922381 @default.
• W1991446568 cites W1993081501 @default.
• W1991446568 cites W1998055274 @default.
• W1991446568 cites W2002872034 @default.
• W1991446568 cites W2010655741 @default.
• W1991446568 cites W2012694727 @default.
• W1991446568 cites W2013156289 @default.
• W1991446568 cites W2015453308 @default.
• W1991446568 cites W2023687292 @default.
• W1991446568 cites W2024907303 @default.
• W1991446568 cites W2031050060 @default.
• W1991446568 cites W2031185644 @default.
• W1991446568 cites W2040714003 @default.
• W1991446568 cites W2048456201 @default.
• W1991446568 cites W2050891199 @default.
• W1991446568 cites W2050932091 @default.
• W1991446568 cites W2052479752 @default.
• W1991446568 cites W2056560999 @default.
• W1991446568 cites W2056579820 @default.
• W1991446568 cites W2074366410 @default.
• W1991446568 cites W2077034347 @default.
• W1991446568 cites W2079100586 @default.
• W1991446568 cites W2085373224 @default.
• W1991446568 cites W2085612857 @default.
• W1991446568 cites W2087497808 @default.
• W1991446568 cites W2099081485 @default.
• W1991446568 cites W2123257463 @default.
• W1991446568 cites W2152331429 @default.
• W1991446568 cites W2156020148 @default.
• W1991446568 cites W2159240333 @default.
• W1991446568 cites W2163792954 @default.
• W1991446568 cites W2326666040 @default.
• W1991446568 cites W4242063371 @default.
• W1991446568 doi "https://doi.org/10.1002/jgm.1125" @default.
• W1991446568 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18044795" @default.
• W1991446568 hasPublicationYear "2007" @default.
• W1991446568 type Work @default.
• W1991446568 sameAs 1991446568 @default.
• W1991446568 citedByCount "71" @default.
• W1991446568 countsByYear W19914465682012 @default.
• W1991446568 countsByYear W19914465682013 @default.
• W1991446568 countsByYear W19914465682014 @default.
• W1991446568 countsByYear W19914465682015 @default.
• W1991446568 countsByYear W19914465682016 @default.
• W1991446568 countsByYear W19914465682017 @default.
• W1991446568 countsByYear W19914465682018 @default.
• W1991446568 countsByYear W19914465682019 @default.
• W1991446568 countsByYear W19914465682021 @default.
• W1991446568 countsByYear W19914465682022 @default.
• W1991446568 countsByYear W19914465682023 @default.
• W1991446568 crossrefType "journal-article" @default.
• W1991446568 hasAuthorship W1991446568A5024772304 @default.
• W1991446568 hasAuthorship W1991446568A5030998615 @default.
• W1991446568 hasAuthorship W1991446568A5061234170 @default.
• W1991446568 hasAuthorship W1991446568A5062744013 @default.
• W1991446568 hasAuthorship W1991446568A5065810893 @default.
• W1991446568 hasConcept C104317684 @default.
• W1991446568 hasConcept C12554922 @default.
• W1991446568 hasConcept C153911025 @default.
• W1991446568 hasConcept C170493617 @default.
• W1991446568 hasConcept C185592680 @default.
• W1991446568 hasConcept C189165786 @default.
• W1991446568 hasConcept C202751555 @default.
• W1991446568 hasConcept C2780932548 @default.
• W1991446568 hasConcept C54009773 @default.
• W1991446568 hasConcept C55493867 @default.
• W1991446568 hasConcept C86803240 @default.
• W1991446568 hasConceptScore W1991446568C104317684 @default.
• W1991446568 hasConceptScore W1991446568C12554922 @default.
• W1991446568 hasConceptScore W1991446568C153911025 @default.
• W1991446568 hasConceptScore W1991446568C170493617 @default.
• W1991446568 hasConceptScore W1991446568C185592680 @default.
• W1991446568 hasConceptScore W1991446568C189165786 @default.
• W1991446568 hasConceptScore W1991446568C202751555 @default.
• W1991446568 hasConceptScore W1991446568C2780932548 @default.
• W1991446568 hasConceptScore W1991446568C54009773 @default.
• W1991446568 hasConceptScore W1991446568C55493867 @default.
• W1991446568 hasConceptScore W1991446568C86803240 @default.
• W1991446568 hasIssue "1" @default.
• W1991446568 hasLocation W19914465681 @default.
• W1991446568 hasLocation W19914465682 @default.
• W1991446568 hasOpenAccess W1991446568 @default.
• W1991446568 hasPrimaryLocation W19914465681 @default.
• W1991446568 hasRelatedWork W2290989990 @default.
• W1991446568 hasRelatedWork W2357524235 @default.
• W1991446568 hasRelatedWork W2357624417 @default.

• next