Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991452337> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W1991452337 endingPage "232" @default.
- W1991452337 startingPage "232" @default.
- W1991452337 abstract "Different from cloned mice and cattle, which are often born with large offspring syndrome (LOS), pigs produced through nuclear transfer have not been found to be overly large compared to their age-matched controls. In our study, significant differences (P < 0.05) were found in the body weight of both deceased newborn cloned piglets (n = 9) (1.2 � 0.8 vs. 2.1 � 0.2 kg) and cloned pigs at one month of age (n = 7) (6.1 � 1.3 vs. 8.0 � 0.8 kg), when compared to their controls (n = 5 for each age group). Because imprinted genes are important regulators of fetal growth and are subject to reprogramming during nuclear transfer, we aimed to determine the expression levels of both growth-enhancing and growth-inhibiting imprinted genes in these cloned pigs by real time quantitative reverse transcription polymerase chain reaction (RT-PCR). These genes include: Igf2 and Peg3 (growth-promoting), and Igf2r and Grb10 (growth-inhibiting). Tissues from six major organs were collected from clones and controls of both age groups. SYBR Green I was utilized as a reporter for the PCR reactions and 18S ribosomal RNA was used as the internal reference for quantifying the relative expression level of all genes. With the exception of Igf2, the other three imprinted genes were found to have statistically different levels of expression in certain organs of both groups of pigs when compared to their age-matched controls. However, no strong correlation was found between the levels of gene expression and the low-body-weight phenotype of the cloned pigs. Contrary to the notion that nuclear transfer can reduce the variation in the resulting animals due to their identical genetic make-up, larger variances of gene expression were found in clones of both groups, indicating the randomness of the nuclear reprogramming process. For all four imprinted genes studied, the coefficient of variation (CV) decreased greatly from newborn to one month old in both clones and controls, suggesting a more stringent regulation on imprinted gene expression after birth." @default.
- W1991452337 created "2016-06-24" @default.
- W1991452337 creator A5004818290 @default.
- W1991452337 creator A5010157169 @default.
- W1991452337 creator A5038423396 @default.
- W1991452337 creator A5043705004 @default.
- W1991452337 creator A5079905863 @default.
- W1991452337 creator A5089751693 @default.
- W1991452337 date "2006-01-01" @default.
- W1991452337 modified "2023-09-27" @default.
- W1991452337 title "249 EXPRESSION OF GROWTH-RELATED IMPRINTED GENES IN DECEASED NEWBORN AND SURVIVING CLONED PIGLETS" @default.
- W1991452337 doi "https://doi.org/10.1071/rdv18n2ab249" @default.
- W1991452337 hasPublicationYear "2006" @default.
- W1991452337 type Work @default.
- W1991452337 sameAs 1991452337 @default.
- W1991452337 citedByCount "0" @default.
- W1991452337 crossrefType "journal-article" @default.
- W1991452337 hasAuthorship W1991452337A5004818290 @default.
- W1991452337 hasAuthorship W1991452337A5010157169 @default.
- W1991452337 hasAuthorship W1991452337A5038423396 @default.
- W1991452337 hasAuthorship W1991452337A5043705004 @default.
- W1991452337 hasAuthorship W1991452337A5079905863 @default.
- W1991452337 hasAuthorship W1991452337A5089751693 @default.
- W1991452337 hasConcept C104317684 @default.
- W1991452337 hasConcept C112672928 @default.
- W1991452337 hasConcept C150194340 @default.
- W1991452337 hasConcept C153911025 @default.
- W1991452337 hasConcept C16685009 @default.
- W1991452337 hasConcept C190727270 @default.
- W1991452337 hasConcept C201492766 @default.
- W1991452337 hasConcept C2779234561 @default.
- W1991452337 hasConcept C48023723 @default.
- W1991452337 hasConcept C54355233 @default.
- W1991452337 hasConcept C71924100 @default.
- W1991452337 hasConcept C77255625 @default.
- W1991452337 hasConcept C86803240 @default.
- W1991452337 hasConcept C94715292 @default.
- W1991452337 hasConceptScore W1991452337C104317684 @default.
- W1991452337 hasConceptScore W1991452337C112672928 @default.
- W1991452337 hasConceptScore W1991452337C150194340 @default.
- W1991452337 hasConceptScore W1991452337C153911025 @default.
- W1991452337 hasConceptScore W1991452337C16685009 @default.
- W1991452337 hasConceptScore W1991452337C190727270 @default.
- W1991452337 hasConceptScore W1991452337C201492766 @default.
- W1991452337 hasConceptScore W1991452337C2779234561 @default.
- W1991452337 hasConceptScore W1991452337C48023723 @default.
- W1991452337 hasConceptScore W1991452337C54355233 @default.
- W1991452337 hasConceptScore W1991452337C71924100 @default.
- W1991452337 hasConceptScore W1991452337C77255625 @default.
- W1991452337 hasConceptScore W1991452337C86803240 @default.
- W1991452337 hasConceptScore W1991452337C94715292 @default.
- W1991452337 hasIssue "2" @default.
- W1991452337 hasLocation W19914523371 @default.
- W1991452337 hasOpenAccess W1991452337 @default.
- W1991452337 hasPrimaryLocation W19914523371 @default.
- W1991452337 hasRelatedWork W1985792087 @default.
- W1991452337 hasRelatedWork W2016058404 @default.
- W1991452337 hasRelatedWork W2071496500 @default.
- W1991452337 hasRelatedWork W2098273174 @default.
- W1991452337 hasRelatedWork W2333089225 @default.
- W1991452337 hasRelatedWork W2596346515 @default.
- W1991452337 hasRelatedWork W2945385425 @default.
- W1991452337 hasRelatedWork W2996351169 @default.
- W1991452337 hasRelatedWork W3101944750 @default.
- W1991452337 hasRelatedWork W4313204077 @default.
- W1991452337 hasVolume "18" @default.
- W1991452337 isParatext "false" @default.
- W1991452337 isRetracted "false" @default.
- W1991452337 magId "1991452337" @default.
- W1991452337 workType "article" @default.