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- W1991493381 abstract "To search for extraterrestrial life surrogate extreme environments on Earth have been chosen for investigation. An example of a surrogate site is the Canadian subpermafrost. Investigations into microbial communities occurred by access fracture borehole water in the Lupin gold mine, and drill rock cores and drilling waters in the High Lake region of Nunavut, Canada. Membrane lipid analyses uses GC/MS and HPLC/ES/MS/MS to provide estimates of biomass, phospholipid (PLFA) and respiratory quinone composition, and compositional changes related to membrane stress caused by nutritional limitations or exposure to toxic conditions. Lupin fracture borehole waters were collected from 800 to 1200 meters, while the High Lake rock cores were collected from 335 to 535 meters. Biomass estimates based on PLFA ranged from 0.25 to 22 pmol L<sup>-1</sup> for the Lupin waters. High Lake drill waters had biomass that ranged from below detection limits (bdl) to 595 pmol/ml, while rock core samples had biomass estimates ranging from bdl to 32 pmol g<sup>-1</sup>. PLFA profiles revealed the presence of both Gram +/- bacteria and sulfatereducing bacteria. Specific PLFA ratios indicate that the bacterial communities were physiologically stressed. Menaquinones were the most abundant but varied in the dominant isoprene units between the two sites. Ubiquinone to menaquinone ratio indicated that these samples have been anoxic for a long time. Methods to detect life signatures at surrogate sites on Earth will be critical for assessing extraterrestrial life. Currently, the membrane lipid analyses provide additional information not easily provided by other molecular techniques." @default.
- W1991493381 created "2016-06-24" @default.
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- W1991493381 date "2008-08-28" @default.
- W1991493381 modified "2023-09-26" @default.
- W1991493381 title "What do membrane lipids tell us about the microorganisms living in extreme environments?" @default.
- W1991493381 doi "https://doi.org/10.1117/12.795378" @default.
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