FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991502666> ?p ?o ?g. }

• W1991502666 endingPage "2764" @default.
• W1991502666 startingPage "2753" @default.
• W1991502666 abstract "The Escherichia coli-infecting bacteriophage T7 encodes a 7 kDa protein, called Gp2, which is a potent inhibitor of the host RNA polymerase (RNAp). Gp2 is essential for T7 phage development. The interaction site for Gp2 on the E. coli RNAp is the β' jaw domain, which is part of the DNA binding channel. The binding of Gp2 to the β' jaw antagonizes several steps associated with interactions between the RNAp and promoter DNA, leading to inhibition of transcription at the open promoter complex formation step. In the structure of the complex formed between Gp2 and a fragment of the β' jaw, amino acid residues in the β3 strand of Gp2 contribute to the primary interaction interface with the β' jaw. The 7009 E. coli strain is resistant to T7 because it carries a charge reversal point mutation in the β' jaw that prevents Gp2 binding. However, a T7 phage encoding a mutant form of Gp2, called Gp2(β), which carries triple amino acid substitutions E24K, F27Y and R56C, can productively infect this strain. By studying the molecular basis of inhibition of RNAp from the 7009 strain by Gp2(β), we provide several lines of evidence that the E24K and F27Y substitutions facilitate an interaction with RNAp when the primary interaction interface with the β' jaw is compromised. The proposed additional interaction interface between RNAp and Gp2 may contribute to the multipronged mechanism of transcription inhibition by Gp2." @default.
• W1991502666 created "2016-06-24" @default.
• W1991502666 creator A5007953356 @default.
• W1991502666 creator A5056880626 @default.
• W1991502666 creator A5056994790 @default.
• W1991502666 creator A5071612869 @default.
• W1991502666 creator A5089901980 @default.
• W1991502666 date "2012-11-01" @default.
• W1991502666 modified "2023-09-27" @default.
• W1991502666 title "Substitutions in the Escherichia coli RNA polymerase inhibitor T7 Gp2 that allow inhibition of transcription when the primary interaction interface between Gp2 and RNA polymerase becomes compromised" @default.
• W1991502666 cites W1584156833 @default.
• W1991502666 cites W1967725326 @default.
• W1991502666 cites W1975941280 @default.
• W1991502666 cites W1982729908 @default.
• W1991502666 cites W2001394772 @default.
• W1991502666 cites W2002043114 @default.
• W1991502666 cites W2009783713 @default.
• W1991502666 cites W2035831750 @default.
• W1991502666 cites W2036758334 @default.
• W1991502666 cites W2039363391 @default.
• W1991502666 cites W2041031058 @default.
• W1991502666 cites W2052094781 @default.
• W1991502666 cites W2054901066 @default.
• W1991502666 cites W2073626488 @default.
• W1991502666 cites W2086785140 @default.
• W1991502666 cites W2104239418 @default.
• W1991502666 cites W2106494572 @default.
• W1991502666 cites W2106593915 @default.
• W1991502666 cites W2142251166 @default.
• W1991502666 cites W2150237825 @default.
• W1991502666 cites W2152301430 @default.
• W1991502666 cites W2162800127 @default.
• W1991502666 cites W2169131251 @default.
• W1991502666 cites W2169156757 @default.
• W1991502666 doi "https://doi.org/10.1099/mic.0.062547-0" @default.
• W1991502666 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3541766" @default.
• W1991502666 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22977089" @default.
• W1991502666 hasPublicationYear "2012" @default.
• W1991502666 type Work @default.
• W1991502666 sameAs 1991502666 @default.
• W1991502666 citedByCount "12" @default.
• W1991502666 countsByYear W19915026662013 @default.
• W1991502666 countsByYear W19915026662017 @default.
• W1991502666 countsByYear W19915026662019 @default.
• W1991502666 countsByYear W19915026662022 @default.
• W1991502666 crossrefType "journal-article" @default.
• W1991502666 hasAuthorship W1991502666A5007953356 @default.
• W1991502666 hasAuthorship W1991502666A5056880626 @default.
• W1991502666 hasAuthorship W1991502666A5056994790 @default.
• W1991502666 hasAuthorship W1991502666A5071612869 @default.
• W1991502666 hasAuthorship W1991502666A5089901980 @default.
• W1991502666 hasBestOaLocation W19915026661 @default.
• W1991502666 hasConcept C104317684 @default.
• W1991502666 hasConcept C107824862 @default.
• W1991502666 hasConcept C138885662 @default.
• W1991502666 hasConcept C143065580 @default.
• W1991502666 hasConcept C153911025 @default.
• W1991502666 hasConcept C176944494 @default.
• W1991502666 hasConcept C179926584 @default.
• W1991502666 hasConcept C2776441376 @default.
• W1991502666 hasConcept C2776449523 @default.
• W1991502666 hasConcept C2780048736 @default.
• W1991502666 hasConcept C41895202 @default.
• W1991502666 hasConcept C54355233 @default.
• W1991502666 hasConcept C547475151 @default.
• W1991502666 hasConcept C552990157 @default.
• W1991502666 hasConcept C67705224 @default.
• W1991502666 hasConcept C82381507 @default.
• W1991502666 hasConcept C86803240 @default.
• W1991502666 hasConceptScore W1991502666C104317684 @default.
• W1991502666 hasConceptScore W1991502666C107824862 @default.
• W1991502666 hasConceptScore W1991502666C138885662 @default.
• W1991502666 hasConceptScore W1991502666C143065580 @default.
• W1991502666 hasConceptScore W1991502666C153911025 @default.
• W1991502666 hasConceptScore W1991502666C176944494 @default.
• W1991502666 hasConceptScore W1991502666C179926584 @default.
• W1991502666 hasConceptScore W1991502666C2776441376 @default.
• W1991502666 hasConceptScore W1991502666C2776449523 @default.
• W1991502666 hasConceptScore W1991502666C2780048736 @default.
• W1991502666 hasConceptScore W1991502666C41895202 @default.
• W1991502666 hasConceptScore W1991502666C54355233 @default.
• W1991502666 hasConceptScore W1991502666C547475151 @default.
• W1991502666 hasConceptScore W1991502666C552990157 @default.
• W1991502666 hasConceptScore W1991502666C67705224 @default.
• W1991502666 hasConceptScore W1991502666C82381507 @default.
• W1991502666 hasConceptScore W1991502666C86803240 @default.
• W1991502666 hasIssue "11" @default.
• W1991502666 hasLocation W19915026661 @default.
• W1991502666 hasLocation W19915026662 @default.
• W1991502666 hasLocation W19915026663 @default.
• W1991502666 hasLocation W19915026664 @default.
• W1991502666 hasLocation W19915026665 @default.
• W1991502666 hasOpenAccess W1991502666 @default.
• W1991502666 hasPrimaryLocation W19915026661 @default.
• W1991502666 hasRelatedWork W1492171871 @default.
• W1991502666 hasRelatedWork W1991502666 @default.
• W1991502666 hasRelatedWork W2022811076 @default.
• W1991502666 hasRelatedWork W2030008758 @default.

• next