Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991519903> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W1991519903 endingPage "180" @default.
- W1991519903 startingPage "171" @default.
- W1991519903 abstract "Skeletal myoblast cell proliferation and subsequent differentiation are dependent on developmentally regulated expression of the fibroblast growth factor receptor 1 (FGFR1) gene. We have previously reported the isolation and initial characterization of the chicken FGFR1 gene (cek1) promoter. Both distal and proximal regions of the promoter were identified as necessary for developmentally regulated transcriptional activity in proliferating myoblasts, including its down-regulation in differentiated muscle fibers in vitro. Here we report detailed characterization of the molecular mechanism regulating FGFR1 promoter activity via the distal promoter region in proliferating myoblasts. This region was identified as a 242 base pair segment located greater than 1 kilobase upstream from the start of transcription that conferred increased transcriptional activity to a minimal thymidine kinase promoter. This segment contains two Sp1 binding sites. Site directed mutagenesis and transfection studies indicated that both Sp1 sites are functional and both are required for FGFR1 promoter activity. Furthermore, Sp1 binding to the two sites was synergistic enhancing FGFR1 promoter activity. The specificity of Sp1 binding to the two distal promoter cis-elements was verified by electromobility shift and transfection assays employing an Sp1 expression construct. Differences in myoblast versus fibroblast-specific protein-DNA complex formation at these sites correlated with high promoter activity in myoblasts and significantly reduced promoter activity in fibroblasts. These studies for the first time establish a molecular mechanism regulating FGFR1 gene expression during myoblast proliferation." @default.
- W1991519903 created "2016-06-24" @default.
- W1991519903 creator A5008740298 @default.
- W1991519903 creator A5009901608 @default.
- W1991519903 date "2001-05-01" @default.
- W1991519903 modified "2023-09-26" @default.
- W1991519903 title "Two distal Sp1-binding cis-elements regulate fibroblast growth factor receptor 1 (FGFR1) gene expression in myoblasts" @default.
- W1991519903 cites W1513451331 @default.
- W1991519903 cites W1837633367 @default.
- W1991519903 cites W1881900550 @default.
- W1991519903 cites W1964556465 @default.
- W1991519903 cites W1967215544 @default.
- W1991519903 cites W1975578266 @default.
- W1991519903 cites W1987485173 @default.
- W1991519903 cites W1998793785 @default.
- W1991519903 cites W2014333872 @default.
- W1991519903 cites W2024612038 @default.
- W1991519903 cites W2029559050 @default.
- W1991519903 cites W2032365199 @default.
- W1991519903 cites W2033899177 @default.
- W1991519903 cites W2037653294 @default.
- W1991519903 cites W2044523536 @default.
- W1991519903 cites W2054523950 @default.
- W1991519903 cites W2058525519 @default.
- W1991519903 cites W2075618050 @default.
- W1991519903 cites W2076875187 @default.
- W1991519903 cites W2080053060 @default.
- W1991519903 cites W2082460645 @default.
- W1991519903 cites W2127966496 @default.
- W1991519903 cites W2161560274 @default.
- W1991519903 cites W2186097846 @default.
- W1991519903 cites W2307290500 @default.
- W1991519903 cites W4250201766 @default.
- W1991519903 doi "https://doi.org/10.1016/s0378-1119(01)00478-4" @default.
- W1991519903 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11404014" @default.
- W1991519903 hasPublicationYear "2001" @default.
- W1991519903 type Work @default.
- W1991519903 sameAs 1991519903 @default.
- W1991519903 citedByCount "22" @default.
- W1991519903 countsByYear W19915199032012 @default.
- W1991519903 countsByYear W19915199032013 @default.
- W1991519903 countsByYear W19915199032017 @default.
- W1991519903 crossrefType "journal-article" @default.
- W1991519903 hasAuthorship W1991519903A5008740298 @default.
- W1991519903 hasAuthorship W1991519903A5009901608 @default.
- W1991519903 hasConcept C101762097 @default.
- W1991519903 hasConcept C104317684 @default.
- W1991519903 hasConcept C150194340 @default.
- W1991519903 hasConcept C153911025 @default.
- W1991519903 hasConcept C165864922 @default.
- W1991519903 hasConcept C170493617 @default.
- W1991519903 hasConcept C207200792 @default.
- W1991519903 hasConcept C49418065 @default.
- W1991519903 hasConcept C54009773 @default.
- W1991519903 hasConcept C54355233 @default.
- W1991519903 hasConcept C57467964 @default.
- W1991519903 hasConcept C74373430 @default.
- W1991519903 hasConcept C86339819 @default.
- W1991519903 hasConcept C86803240 @default.
- W1991519903 hasConcept C95444343 @default.
- W1991519903 hasConceptScore W1991519903C101762097 @default.
- W1991519903 hasConceptScore W1991519903C104317684 @default.
- W1991519903 hasConceptScore W1991519903C150194340 @default.
- W1991519903 hasConceptScore W1991519903C153911025 @default.
- W1991519903 hasConceptScore W1991519903C165864922 @default.
- W1991519903 hasConceptScore W1991519903C170493617 @default.
- W1991519903 hasConceptScore W1991519903C207200792 @default.
- W1991519903 hasConceptScore W1991519903C49418065 @default.
- W1991519903 hasConceptScore W1991519903C54009773 @default.
- W1991519903 hasConceptScore W1991519903C54355233 @default.
- W1991519903 hasConceptScore W1991519903C57467964 @default.
- W1991519903 hasConceptScore W1991519903C74373430 @default.
- W1991519903 hasConceptScore W1991519903C86339819 @default.
- W1991519903 hasConceptScore W1991519903C86803240 @default.
- W1991519903 hasConceptScore W1991519903C95444343 @default.
- W1991519903 hasIssue "1-2" @default.
- W1991519903 hasLocation W19915199031 @default.
- W1991519903 hasLocation W19915199032 @default.
- W1991519903 hasOpenAccess W1991519903 @default.
- W1991519903 hasPrimaryLocation W19915199031 @default.
- W1991519903 hasRelatedWork W1983163840 @default.
- W1991519903 hasRelatedWork W1985189742 @default.
- W1991519903 hasRelatedWork W2009966535 @default.
- W1991519903 hasRelatedWork W2111182449 @default.
- W1991519903 hasRelatedWork W2121924440 @default.
- W1991519903 hasRelatedWork W2125934144 @default.
- W1991519903 hasRelatedWork W2140331807 @default.
- W1991519903 hasRelatedWork W2363432561 @default.
- W1991519903 hasRelatedWork W2806835158 @default.
- W1991519903 hasRelatedWork W4299592906 @default.
- W1991519903 hasVolume "270" @default.
- W1991519903 isParatext "false" @default.
- W1991519903 isRetracted "false" @default.
- W1991519903 magId "1991519903" @default.
- W1991519903 workType "article" @default.