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- W199152904 endingPage "345" @default.
- W199152904 startingPage "339" @default.
- W199152904 abstract "利用ＲＴ－ＰＣＲ方法，从小鼠肝脏组织总ＲＮＡ中扩增出４．５ＳＲＮＡ的ｃＤＮＡ。该ｃＤＮＡ被克隆到ｐＧＥＭ３Ｚｆ（＋）质粒上，酶切鉴定并测序。然后将该序列插入以Ｌｕｃ基因作为报道基因的表达载体ｐＳＶｌｕｃ２０的ＰｖｕⅡ位点，构建了含４．２ＳＲＮＡ逆转座子的表达载体ｐＳＶｌｕｃ２０－４．５Ｓ。脂质转染法将表达载体导入小鼠骨髓瘤细胞ＮＳ－１、ＳＰ２／０和人乳腺癌细胞Ｂｃａ６１。结果表明，小鼠４．５ＳＲＮ" @default.
- W199152904 created "2016-06-24" @default.
- W199152904 creator A5004722315 @default.
- W199152904 creator A5070796695 @default.
- W199152904 date "2000-01-01" @default.
- W199152904 modified "2023-09-24" @default.
- W199152904 title "小鼠4.5S RNA逆转座子对Luc基因表达的影响" @default.
- W199152904 hasPublicationYear "2000" @default.
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