Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991582050> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W1991582050 endingPage "35" @default.
- W1991582050 startingPage "31" @default.
- W1991582050 abstract "Leishmaniasis is a group of endemic diseases produced by infection with Leishmania parasites and affects tropical and subtropical regions of the world. Due to the severe problems related to the treatment of this condition (resistance and toxicity), further studies are needed to evaluate new antileishmanial compounds. The activity of antileishmanial prototypes should be analyzed in models that allow a better interpretation of the findings with respect to natural infection. In this sense, the use of an infection model with macrophages and dendritic cells is a better than using promastigotes alone, in order to establish the potential leishmanicidal activity of a prototype compound. For infection analysis, staining with polychromatic dyes such as Giemsa plus microscopic examination is the gold standard. However, it is common to find problems associated with color uniformity, expertise of the observer, sensitivity and specificity of the technique. For this reason, it's necessary to develop tools and protocols to overcome such limitations. This study assessed the utility of the SYBR® Safe fluorescent dye, considering its affinity for nucleic acids as a useful property for staining the nucleus and kinetoplast of Leishmania parasites within an infected cell. Infection (and subsequent treatment) assays were performed in dendritic cells and macrophages infected with Leishmania panamensis parasites to compare SYBR® Safe and Giemsa stain for the same assay. Correlation coefficients were found to be above 0.9 for both techniques; however, unlike Giemsa, SYBR® Safe staining was easier and provided a clearer observation of internalized parasites. These results support the use of SYBR® Safe as a promising tool for evaluating potential antileishmanials given its advantages over the traditional technique." @default.
- W1991582050 created "2016-06-24" @default.
- W1991582050 creator A5002697738 @default.
- W1991582050 creator A5019654996 @default.
- W1991582050 creator A5035918798 @default.
- W1991582050 date "2011-09-01" @default.
- W1991582050 modified "2023-10-14" @default.
- W1991582050 title "Use of a fluorescent stain for evaluating in vitro infection with Leishmania panamensis" @default.
- W1991582050 cites W1997545346 @default.
- W1991582050 cites W2004163475 @default.
- W1991582050 cites W2008501990 @default.
- W1991582050 cites W2025076614 @default.
- W1991582050 cites W2064640589 @default.
- W1991582050 cites W2083127413 @default.
- W1991582050 cites W2100433490 @default.
- W1991582050 cites W2109803080 @default.
- W1991582050 cites W2127052921 @default.
- W1991582050 cites W2152686523 @default.
- W1991582050 cites W2167117483 @default.
- W1991582050 cites W2171950352 @default.
- W1991582050 doi "https://doi.org/10.1016/j.exppara.2011.05.022" @default.
- W1991582050 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21684278" @default.
- W1991582050 hasPublicationYear "2011" @default.
- W1991582050 type Work @default.
- W1991582050 sameAs 1991582050 @default.
- W1991582050 citedByCount "8" @default.
- W1991582050 countsByYear W19915820502012 @default.
- W1991582050 countsByYear W19915820502013 @default.
- W1991582050 countsByYear W19915820502017 @default.
- W1991582050 countsByYear W19915820502020 @default.
- W1991582050 countsByYear W19915820502022 @default.
- W1991582050 countsByYear W19915820502023 @default.
- W1991582050 crossrefType "journal-article" @default.
- W1991582050 hasAuthorship W1991582050A5002697738 @default.
- W1991582050 hasAuthorship W1991582050A5019654996 @default.
- W1991582050 hasAuthorship W1991582050A5035918798 @default.
- W1991582050 hasConcept C104317684 @default.
- W1991582050 hasConcept C136764020 @default.
- W1991582050 hasConcept C154488023 @default.
- W1991582050 hasConcept C159047783 @default.
- W1991582050 hasConcept C203014093 @default.
- W1991582050 hasConcept C2776555147 @default.
- W1991582050 hasConcept C2780968160 @default.
- W1991582050 hasConcept C2781092759 @default.
- W1991582050 hasConcept C2781294515 @default.
- W1991582050 hasConcept C41008148 @default.
- W1991582050 hasConcept C49105822 @default.
- W1991582050 hasConcept C54355233 @default.
- W1991582050 hasConcept C55493867 @default.
- W1991582050 hasConcept C71928629 @default.
- W1991582050 hasConcept C74864618 @default.
- W1991582050 hasConcept C86803240 @default.
- W1991582050 hasConcept C89423630 @default.
- W1991582050 hasConceptScore W1991582050C104317684 @default.
- W1991582050 hasConceptScore W1991582050C136764020 @default.
- W1991582050 hasConceptScore W1991582050C154488023 @default.
- W1991582050 hasConceptScore W1991582050C159047783 @default.
- W1991582050 hasConceptScore W1991582050C203014093 @default.
- W1991582050 hasConceptScore W1991582050C2776555147 @default.
- W1991582050 hasConceptScore W1991582050C2780968160 @default.
- W1991582050 hasConceptScore W1991582050C2781092759 @default.
- W1991582050 hasConceptScore W1991582050C2781294515 @default.
- W1991582050 hasConceptScore W1991582050C41008148 @default.
- W1991582050 hasConceptScore W1991582050C49105822 @default.
- W1991582050 hasConceptScore W1991582050C54355233 @default.
- W1991582050 hasConceptScore W1991582050C55493867 @default.
- W1991582050 hasConceptScore W1991582050C71928629 @default.
- W1991582050 hasConceptScore W1991582050C74864618 @default.
- W1991582050 hasConceptScore W1991582050C86803240 @default.
- W1991582050 hasConceptScore W1991582050C89423630 @default.
- W1991582050 hasIssue "1" @default.
- W1991582050 hasLocation W19915820501 @default.
- W1991582050 hasLocation W19915820502 @default.
- W1991582050 hasOpenAccess W1991582050 @default.
- W1991582050 hasPrimaryLocation W19915820501 @default.
- W1991582050 hasRelatedWork W1991582050 @default.
- W1991582050 hasRelatedWork W2017804562 @default.
- W1991582050 hasRelatedWork W2027589434 @default.
- W1991582050 hasRelatedWork W2063666075 @default.
- W1991582050 hasRelatedWork W2109408368 @default.
- W1991582050 hasRelatedWork W2157193465 @default.
- W1991582050 hasRelatedWork W2366813557 @default.
- W1991582050 hasRelatedWork W2892346699 @default.
- W1991582050 hasRelatedWork W2951897516 @default.
- W1991582050 hasRelatedWork W4298158922 @default.
- W1991582050 hasVolume "129" @default.
- W1991582050 isParatext "false" @default.
- W1991582050 isRetracted "false" @default.
- W1991582050 magId "1991582050" @default.
- W1991582050 workType "article" @default.